Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS15350

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS15340 and RS_RS15345 are separated by 190 nucleotidesRS_RS15345 and RS_RS15350 overlap by 26 nucleotidesRS_RS15350 and RS_RS15355 overlap by 14 nucleotidesRS_RS15355 and RS_RS15360 are separated by 56 nucleotides RS_RS15340: RS_RS15340 - hypothetical protein, at 3,291,074 to 3,291,445 _RS15340 RS_RS15345: RS_RS15345 - DUF1415 domain-containing protein, at 3,291,636 to 3,292,262 _RS15345 RS_RS15350: RS_RS15350 - PIN domain-containing protein, at 3,292,237 to 3,292,650 _RS15350 RS_RS15355: RS_RS15355 - AbrB/MazE/SpoVT family DNA-binding domain-containing protein, at 3,292,637 to 3,292,870 _RS15355 RS_RS15360: RS_RS15360 - glucan biosynthesis protein D, at 3,292,927 to 3,294,534 _RS15360 Position (kb) 3292 3293Strain fitness (log2 ratio) -1 0 1at 3291.603 kb on + strandat 3291.603 kb on + strandat 3291.604 kb on - strandat 3292.170 kb on + strand, within RS_RS15345at 3292.170 kb on + strand, within RS_RS15345at 3292.170 kb on + strand, within RS_RS15345at 3292.170 kb on + strand, within RS_RS15345at 3292.170 kb on + strand, within RS_RS15345at 3292.170 kb on + strand, within RS_RS15345at 3292.170 kb on + strand, within RS_RS15345at 3292.170 kb on + strand, within RS_RS15345at 3292.170 kb on + strand, within RS_RS15345at 3292.171 kb on - strand, within RS_RS15345at 3292.171 kb on - strand, within RS_RS15345at 3292.171 kb on - strand, within RS_RS15345at 3292.171 kb on - strand, within RS_RS15345at 3292.171 kb on - strand, within RS_RS15345at 3292.171 kb on - strand, within RS_RS15345at 3292.302 kb on + strand, within RS_RS15350at 3292.313 kb on + strand, within RS_RS15350at 3292.313 kb on + strand, within RS_RS15350at 3292.313 kb on + strand, within RS_RS15350at 3292.364 kb on + strand, within RS_RS15350at 3292.365 kb on - strand, within RS_RS15350at 3292.365 kb on - strand, within RS_RS15350at 3292.365 kb on - strand, within RS_RS15350at 3292.528 kb on + strand, within RS_RS15350at 3292.528 kb on + strand, within RS_RS15350at 3292.528 kb on + strand, within RS_RS15350at 3292.529 kb on - strand, within RS_RS15350at 3292.529 kb on - strand, within RS_RS15350at 3292.535 kb on - strand, within RS_RS15350at 3292.535 kb on - strand, within RS_RS15350at 3292.535 kb on - strand, within RS_RS15350at 3292.607 kb on + strand, within RS_RS15350at 3292.607 kb on + strand, within RS_RS15350at 3292.608 kb on - strand, within RS_RS15350at 3292.608 kb on - strand, within RS_RS15350at 3292.608 kb on - strand, within RS_RS15350at 3292.913 kb on + strandat 3292.913 kb on + strandat 3292.914 kb on - strandat 3292.916 kb on - strandat 3292.928 kb on + strandat 3292.928 kb on + strandat 3292.929 kb on - strandat 3292.929 kb on - strandat 3292.929 kb on - strandat 3292.961 kb on + strandat 3293.104 kb on + strand, within RS_RS15360at 3293.468 kb on + strand, within RS_RS15360at 3293.469 kb on - strand, within RS_RS15360

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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3,291,603 + -1.2
3,291,603 + -0.2
3,291,604 - +0.0
3,292,170 + RS_RS15345 0.85 -1.2
3,292,170 + RS_RS15345 0.85 +0.1
3,292,170 + RS_RS15345 0.85 +0.2
3,292,170 + RS_RS15345 0.85 -0.1
3,292,170 + RS_RS15345 0.85 +0.1
3,292,170 + RS_RS15345 0.85 -0.4
3,292,170 + RS_RS15345 0.85 -0.4
3,292,170 + RS_RS15345 0.85 +0.1
3,292,170 + RS_RS15345 0.85 -0.0
3,292,171 - RS_RS15345 0.85 -0.1
3,292,171 - RS_RS15345 0.85 +0.5
3,292,171 - RS_RS15345 0.85 +1.2
3,292,171 - RS_RS15345 0.85 -0.4
3,292,171 - RS_RS15345 0.85 -0.3
3,292,171 - RS_RS15345 0.85 -0.0
3,292,302 + RS_RS15350 0.16 +1.7
3,292,313 + RS_RS15350 0.18 +0.6
3,292,313 + RS_RS15350 0.18 +0.2
3,292,313 + RS_RS15350 0.18 -0.3
3,292,364 + RS_RS15350 0.31 -1.4
3,292,365 - RS_RS15350 0.31 -0.0
3,292,365 - RS_RS15350 0.31 -0.8
3,292,365 - RS_RS15350 0.31 +0.6
3,292,528 + RS_RS15350 0.70 +1.2
3,292,528 + RS_RS15350 0.70 +1.2
3,292,528 + RS_RS15350 0.70 -0.9
3,292,529 - RS_RS15350 0.71 -0.1
3,292,529 - RS_RS15350 0.71 -0.3
3,292,535 - RS_RS15350 0.72 -0.4
3,292,535 - RS_RS15350 0.72 +0.2
3,292,535 - RS_RS15350 0.72 -1.4
3,292,607 + RS_RS15350 0.89 +0.7
3,292,607 + RS_RS15350 0.89 -0.4
3,292,608 - RS_RS15350 0.90 +0.1
3,292,608 - RS_RS15350 0.90 +1.2
3,292,608 - RS_RS15350 0.90 +0.1
3,292,913 + -0.1
3,292,913 + +0.1
3,292,914 - -0.5
3,292,916 - -0.2
3,292,928 + +0.1
3,292,928 + -0.2
3,292,929 - -0.1
3,292,929 - -0.5
3,292,929 - -0.3
3,292,961 + +0.7
3,293,104 + RS_RS15360 0.11 -0.4
3,293,468 + RS_RS15360 0.34 +0.4
3,293,469 - RS_RS15360 0.34 +1.2

Or see this region's nucleotide sequence