Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS15320

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS15310 and RS_RS15315 overlap by 4 nucleotidesRS_RS15315 and RS_RS15320 overlap by 8 nucleotidesRS_RS15320 and RS_RS15325 are separated by 22 nucleotidesRS_RS15325 and RS_RS15330 are separated by 156 nucleotides RS_RS15310: RS_RS15310 - ABC transporter ATP-binding protein, at 3,284,345 to 3,285,442 _RS15310 RS_RS15315: RS_RS15315 - sugar ABC transporter permease, at 3,285,439 to 3,286,329 _RS15315 RS_RS15320: RS_RS15320 - carbohydrate ABC transporter permease, at 3,286,322 to 3,287,152 _RS15320 RS_RS15325: RS_RS15325 - membrane protein, at 3,287,175 to 3,287,456 _RS15325 RS_RS15330: RS_RS15330 - carbohydrate ABC transporter substrate-binding protein, at 3,287,613 to 3,289,355 _RS15330 Position (kb) 3286 3287 3288Strain fitness (log2 ratio) -2 -1 0 1at 3285.410 kb on + strandat 3285.410 kb on + strandat 3285.410 kb on + strandat 3285.410 kb on + strandat 3285.413 kb on + strandat 3285.413 kb on + strandat 3285.414 kb on - strandat 3285.538 kb on + strand, within RS_RS15315at 3285.538 kb on + strand, within RS_RS15315at 3285.862 kb on + strand, within RS_RS15315at 3285.863 kb on - strand, within RS_RS15315at 3285.868 kb on + strand, within RS_RS15315at 3285.868 kb on + strand, within RS_RS15315at 3285.869 kb on - strand, within RS_RS15315at 3285.952 kb on + strand, within RS_RS15315at 3285.953 kb on - strand, within RS_RS15315at 3285.985 kb on + strand, within RS_RS15315at 3285.986 kb on - strand, within RS_RS15315at 3285.986 kb on - strand, within RS_RS15315at 3285.986 kb on - strand, within RS_RS15315at 3286.034 kb on - strand, within RS_RS15315at 3286.034 kb on - strand, within RS_RS15315at 3286.108 kb on + strand, within RS_RS15315at 3286.171 kb on + strand, within RS_RS15315at 3286.172 kb on - strand, within RS_RS15315at 3286.172 kb on - strand, within RS_RS15315at 3286.234 kb on + strand, within RS_RS15315at 3286.270 kb on + strandat 3286.270 kb on + strandat 3286.270 kb on + strandat 3286.270 kb on + strandat 3286.271 kb on - strandat 3286.271 kb on - strandat 3286.272 kb on + strandat 3286.272 kb on + strandat 3286.272 kb on + strandat 3286.273 kb on - strandat 3286.273 kb on - strandat 3286.327 kb on + strandat 3286.328 kb on - strandat 3286.385 kb on + strandat 3286.385 kb on + strandat 3286.386 kb on - strandat 3286.386 kb on - strandat 3286.499 kb on + strand, within RS_RS15320at 3286.499 kb on + strand, within RS_RS15320at 3286.499 kb on + strand, within RS_RS15320at 3286.500 kb on - strand, within RS_RS15320at 3286.500 kb on - strand, within RS_RS15320at 3286.500 kb on - strand, within RS_RS15320at 3286.500 kb on - strand, within RS_RS15320at 3286.530 kb on - strand, within RS_RS15320at 3286.539 kb on - strand, within RS_RS15320at 3286.539 kb on - strand, within RS_RS15320at 3286.539 kb on - strand, within RS_RS15320at 3286.557 kb on - strand, within RS_RS15320at 3286.608 kb on - strand, within RS_RS15320at 3286.608 kb on - strand, within RS_RS15320at 3286.715 kb on + strand, within RS_RS15320at 3286.716 kb on - strand, within RS_RS15320at 3286.860 kb on - strand, within RS_RS15320at 3287.117 kb on + strandat 3287.117 kb on + strandat 3287.118 kb on - strandat 3287.118 kb on - strandat 3287.118 kb on - strandat 3287.352 kb on + strand, within RS_RS15325at 3287.353 kb on - strand, within RS_RS15325at 3287.598 kb on + strandat 3287.608 kb on + strandat 3287.608 kb on + strandat 3287.609 kb on - strandat 3287.994 kb on + strand, within RS_RS15330at 3287.995 kb on - strand, within RS_RS15330at 3287.995 kb on - strand, within RS_RS15330at 3287.995 kb on - strand, within RS_RS15330at 3288.036 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.070 kb on - strand, within RS_RS15330at 3288.070 kb on - strand, within RS_RS15330at 3288.070 kb on - strand, within RS_RS15330

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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3,285,410 + -0.2
3,285,410 + +0.9
3,285,410 + -0.3
3,285,410 + -0.0
3,285,413 + -0.1
3,285,413 + -0.3
3,285,414 - -0.2
3,285,538 + RS_RS15315 0.11 -0.3
3,285,538 + RS_RS15315 0.11 -0.3
3,285,862 + RS_RS15315 0.47 -0.4
3,285,863 - RS_RS15315 0.48 -1.2
3,285,868 + RS_RS15315 0.48 -0.2
3,285,868 + RS_RS15315 0.48 -0.3
3,285,869 - RS_RS15315 0.48 -0.3
3,285,952 + RS_RS15315 0.58 -0.7
3,285,953 - RS_RS15315 0.58 +0.2
3,285,985 + RS_RS15315 0.61 -0.2
3,285,986 - RS_RS15315 0.61 +1.2
3,285,986 - RS_RS15315 0.61 -0.7
3,285,986 - RS_RS15315 0.61 -0.1
3,286,034 - RS_RS15315 0.67 -0.6
3,286,034 - RS_RS15315 0.67 -0.7
3,286,108 + RS_RS15315 0.75 -0.0
3,286,171 + RS_RS15315 0.82 +0.0
3,286,172 - RS_RS15315 0.82 -0.5
3,286,172 - RS_RS15315 0.82 +1.2
3,286,234 + RS_RS15315 0.89 +0.5
3,286,270 + -0.4
3,286,270 + -0.0
3,286,270 + -0.3
3,286,270 + +0.2
3,286,271 - -1.7
3,286,271 - +1.2
3,286,272 + +0.3
3,286,272 + +0.4
3,286,272 + -1.2
3,286,273 - +0.6
3,286,273 - -0.4
3,286,327 + -0.3
3,286,328 - -0.3
3,286,385 + -0.1
3,286,385 + -0.8
3,286,386 - -0.3
3,286,386 - -0.2
3,286,499 + RS_RS15320 0.21 -0.4
3,286,499 + RS_RS15320 0.21 -0.2
3,286,499 + RS_RS15320 0.21 -2.0
3,286,500 - RS_RS15320 0.21 +1.0
3,286,500 - RS_RS15320 0.21 +0.2
3,286,500 - RS_RS15320 0.21 +0.3
3,286,500 - RS_RS15320 0.21 -0.4
3,286,530 - RS_RS15320 0.25 -0.4
3,286,539 - RS_RS15320 0.26 -0.0
3,286,539 - RS_RS15320 0.26 -0.2
3,286,539 - RS_RS15320 0.26 +0.2
3,286,557 - RS_RS15320 0.28 +1.2
3,286,608 - RS_RS15320 0.34 -0.2
3,286,608 - RS_RS15320 0.34 +0.3
3,286,715 + RS_RS15320 0.47 -0.6
3,286,716 - RS_RS15320 0.47 -0.5
3,286,860 - RS_RS15320 0.65 +1.2
3,287,117 + -0.5
3,287,117 + +0.1
3,287,118 - -0.4
3,287,118 - -0.3
3,287,118 - -0.2
3,287,352 + RS_RS15325 0.63 -0.3
3,287,353 - RS_RS15325 0.63 -1.8
3,287,598 + -0.3
3,287,608 + -0.3
3,287,608 + +0.6
3,287,609 - +0.3
3,287,994 + RS_RS15330 0.22 -0.6
3,287,995 - RS_RS15330 0.22 -0.4
3,287,995 - RS_RS15330 0.22 -0.4
3,287,995 - RS_RS15330 0.22 -0.3
3,288,036 + RS_RS15330 0.24 -1.1
3,288,069 + RS_RS15330 0.26 +1.2
3,288,069 + RS_RS15330 0.26 -0.4
3,288,069 + RS_RS15330 0.26 -0.4
3,288,069 + RS_RS15330 0.26 -0.3
3,288,069 + RS_RS15330 0.26 -0.2
3,288,069 + RS_RS15330 0.26 -0.4
3,288,070 - RS_RS15330 0.26 -1.0
3,288,070 - RS_RS15330 0.26 -0.5
3,288,070 - RS_RS15330 0.26 -0.4

Or see this region's nucleotide sequence