Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS14020

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS14015 and RS_RS14020 are separated by 105 nucleotidesRS_RS14020 and RS_RS14025 are separated by 150 nucleotides RS_RS14015: RS_RS14015 - DUF2817 domain-containing protein, at 3,012,936 to 3,014,018 _RS14015 RS_RS14020: RS_RS14020 - ribonucleotide-diphosphate reductase subunit beta, at 3,014,124 to 3,015,326 _RS14020 RS_RS14025: RS_RS14025 - ribonucleoside-diphosphate reductase subunit alpha, at 3,015,477 to 3,018,440 _RS14025 Position (kb) 3014 3015 3016Strain fitness (log2 ratio) -1 0 1at 3013.155 kb on - strand, within RS_RS14015at 3013.447 kb on + strand, within RS_RS14015at 3013.526 kb on - strand, within RS_RS14015at 3013.568 kb on + strand, within RS_RS14015at 3013.598 kb on + strand, within RS_RS14015at 3013.599 kb on - strand, within RS_RS14015at 3013.987 kb on + strandat 3015.478 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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3,013,155 - RS_RS14015 0.20 -0.4
3,013,447 + RS_RS14015 0.47 -0.1
3,013,526 - RS_RS14015 0.54 -0.5
3,013,568 + RS_RS14015 0.58 -1.0
3,013,598 + RS_RS14015 0.61 -0.2
3,013,599 - RS_RS14015 0.61 +0.2
3,013,987 + -1.0
3,015,478 + +1.0

Or see this region's nucleotide sequence