Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS13945

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS13940 and RS_RS13945 are separated by 14 nucleotidesRS_RS13945 and RS_RS13950 are separated by 86 nucleotides RS_RS13940: RS_RS13940 - acetyl-CoA carboxylase biotin carboxylase subunit, at 2,997,201 to 2,998,571 _RS13940 RS_RS13945: RS_RS13945 - 50S ribosomal protein L11 methyltransferase, at 2,998,586 to 2,999,482 _RS13945 RS_RS13950: RS_RS13950 - DUF3426 domain-containing protein, at 2,999,569 to 3,000,990 _RS13950 Position (kb) 2998 2999 3000Strain fitness (log2 ratio) -1 0 1at 2998.859 kb on + strand, within RS_RS13945at 2998.927 kb on + strand, within RS_RS13945at 2999.201 kb on + strand, within RS_RS13945at 2999.614 kb on - strandat 2999.722 kb on - strand, within RS_RS13950at 3000.119 kb on + strand, within RS_RS13950at 3000.310 kb on - strand, within RS_RS13950

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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2,998,859 + RS_RS13945 0.30 +0.2
2,998,927 + RS_RS13945 0.38 -0.3
2,999,201 + RS_RS13945 0.69 -0.7
2,999,614 - +0.2
2,999,722 - RS_RS13950 0.11 +1.0
3,000,119 + RS_RS13950 0.39 +0.9
3,000,310 - RS_RS13950 0.52 +0.4

Or see this region's nucleotide sequence