Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS11305 and RS_RS11310 are separated by 22 nucleotides RS_RS11310 and RS_RS11315 overlap by 4 nucleotides RS_RS11315 and RS_RS11320 are separated by 314 nucleotides
RS_RS11305: RS_RS11305 - 3-oxoadipyl-CoA thiolase, at 2,444,630 to 2,445,832
_RS11305
RS_RS11310: RS_RS11310 - CoA transferase subunit B, at 2,445,855 to 2,446,517
_RS11310
RS_RS11315: RS_RS11315 - 3-oxoadipate CoA-transferase subunit A, at 2,446,514 to 2,447,203
_RS11315
RS_RS11320: RS_RS11320 - IclR family transcriptional regulator, at 2,447,518 to 2,448,309
_RS11320
Position (kb)
2446
2447
2448 Strain fitness (log2 ratio)
-1
0
1 at 2445.818 kb on - strand at 2445.973 kb on + strand, within RS_RS11310 at 2445.973 kb on + strand, within RS_RS11310 at 2445.973 kb on + strand, within RS_RS11310 at 2445.981 kb on - strand, within RS_RS11310 at 2446.195 kb on + strand, within RS_RS11310 at 2446.196 kb on - strand, within RS_RS11310 at 2446.246 kb on + strand, within RS_RS11310 at 2446.695 kb on + strand, within RS_RS11315 at 2446.695 kb on + strand, within RS_RS11315 at 2446.773 kb on + strand, within RS_RS11315 at 2446.774 kb on - strand, within RS_RS11315 at 2446.950 kb on + strand, within RS_RS11315 at 2446.950 kb on + strand, within RS_RS11315 at 2446.950 kb on + strand, within RS_RS11315 at 2446.951 kb on - strand, within RS_RS11315 at 2446.951 kb on - strand, within RS_RS11315 at 2446.951 kb on - strand, within RS_RS11315 at 2446.951 kb on - strand, within RS_RS11315 at 2446.951 kb on - strand, within RS_RS11315 at 2447.474 kb on + strand at 2447.475 kb on - strand at 2447.560 kb on + strand at 2447.561 kb on - strand at 2447.561 kb on - strand at 2447.561 kb on - strand at 2447.561 kb on - strand at 2447.561 kb on - strand at 2447.771 kb on - strand, within RS_RS11320 at 2447.881 kb on + strand, within RS_RS11320 at 2447.881 kb on + strand, within RS_RS11320 at 2447.882 kb on - strand, within RS_RS11320 at 2447.882 kb on - strand, within RS_RS11320
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 2,445,818 - +0.3 2,445,973 + RS_RS11310 0.18 -0.2 2,445,973 + RS_RS11310 0.18 -0.4 2,445,973 + RS_RS11310 0.18 +0.4 2,445,981 - RS_RS11310 0.19 -0.2 2,446,195 + RS_RS11310 0.51 +0.2 2,446,196 - RS_RS11310 0.51 +0.9 2,446,246 + RS_RS11310 0.59 +0.1 2,446,695 + RS_RS11315 0.26 +0.1 2,446,695 + RS_RS11315 0.26 +0.9 2,446,773 + RS_RS11315 0.38 -0.6 2,446,774 - RS_RS11315 0.38 -1.6 2,446,950 + RS_RS11315 0.63 +0.9 2,446,950 + RS_RS11315 0.63 -0.2 2,446,950 + RS_RS11315 0.63 +0.3 2,446,951 - RS_RS11315 0.63 -0.1 2,446,951 - RS_RS11315 0.63 +0.2 2,446,951 - RS_RS11315 0.63 +0.9 2,446,951 - RS_RS11315 0.63 -1.1 2,446,951 - RS_RS11315 0.63 -0.1 2,447,474 + +0.8 2,447,475 - +0.1 2,447,560 + +0.5 2,447,561 - -0.1 2,447,561 - -0.0 2,447,561 - +0.2 2,447,561 - -0.1 2,447,561 - +0.1 2,447,771 - RS_RS11320 0.32 -0.5 2,447,881 + RS_RS11320 0.46 +1.0 2,447,881 + RS_RS11320 0.46 -0.7 2,447,882 - RS_RS11320 0.46 +0.8 2,447,882 - RS_RS11320 0.46 -0.1
Or see this region's nucleotide sequence