Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS11315

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS11305 and RS_RS11310 are separated by 22 nucleotidesRS_RS11310 and RS_RS11315 overlap by 4 nucleotidesRS_RS11315 and RS_RS11320 are separated by 314 nucleotides RS_RS11305: RS_RS11305 - 3-oxoadipyl-CoA thiolase, at 2,444,630 to 2,445,832 _RS11305 RS_RS11310: RS_RS11310 - CoA transferase subunit B, at 2,445,855 to 2,446,517 _RS11310 RS_RS11315: RS_RS11315 - 3-oxoadipate CoA-transferase subunit A, at 2,446,514 to 2,447,203 _RS11315 RS_RS11320: RS_RS11320 - IclR family transcriptional regulator, at 2,447,518 to 2,448,309 _RS11320 Position (kb) 2446 2447 2448Strain fitness (log2 ratio) -1 0 1at 2445.818 kb on - strandat 2445.973 kb on + strand, within RS_RS11310at 2445.973 kb on + strand, within RS_RS11310at 2445.973 kb on + strand, within RS_RS11310at 2445.981 kb on - strand, within RS_RS11310at 2446.195 kb on + strand, within RS_RS11310at 2446.196 kb on - strand, within RS_RS11310at 2446.246 kb on + strand, within RS_RS11310at 2446.695 kb on + strand, within RS_RS11315at 2446.695 kb on + strand, within RS_RS11315at 2446.773 kb on + strand, within RS_RS11315at 2446.774 kb on - strand, within RS_RS11315at 2446.950 kb on + strand, within RS_RS11315at 2446.950 kb on + strand, within RS_RS11315at 2446.950 kb on + strand, within RS_RS11315at 2446.951 kb on - strand, within RS_RS11315at 2446.951 kb on - strand, within RS_RS11315at 2446.951 kb on - strand, within RS_RS11315at 2446.951 kb on - strand, within RS_RS11315at 2446.951 kb on - strand, within RS_RS11315at 2447.474 kb on + strandat 2447.475 kb on - strandat 2447.560 kb on + strandat 2447.561 kb on - strandat 2447.561 kb on - strandat 2447.561 kb on - strandat 2447.561 kb on - strandat 2447.561 kb on - strandat 2447.771 kb on - strand, within RS_RS11320at 2447.881 kb on + strand, within RS_RS11320at 2447.881 kb on + strand, within RS_RS11320at 2447.882 kb on - strand, within RS_RS11320at 2447.882 kb on - strand, within RS_RS11320

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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2,445,818 - +0.3
2,445,973 + RS_RS11310 0.18 -0.2
2,445,973 + RS_RS11310 0.18 -0.4
2,445,973 + RS_RS11310 0.18 +0.4
2,445,981 - RS_RS11310 0.19 -0.2
2,446,195 + RS_RS11310 0.51 +0.2
2,446,196 - RS_RS11310 0.51 +0.9
2,446,246 + RS_RS11310 0.59 +0.1
2,446,695 + RS_RS11315 0.26 +0.1
2,446,695 + RS_RS11315 0.26 +0.9
2,446,773 + RS_RS11315 0.38 -0.6
2,446,774 - RS_RS11315 0.38 -1.6
2,446,950 + RS_RS11315 0.63 +0.9
2,446,950 + RS_RS11315 0.63 -0.2
2,446,950 + RS_RS11315 0.63 +0.3
2,446,951 - RS_RS11315 0.63 -0.1
2,446,951 - RS_RS11315 0.63 +0.2
2,446,951 - RS_RS11315 0.63 +0.9
2,446,951 - RS_RS11315 0.63 -1.1
2,446,951 - RS_RS11315 0.63 -0.1
2,447,474 + +0.8
2,447,475 - +0.1
2,447,560 + +0.5
2,447,561 - -0.1
2,447,561 - -0.0
2,447,561 - +0.2
2,447,561 - -0.1
2,447,561 - +0.1
2,447,771 - RS_RS11320 0.32 -0.5
2,447,881 + RS_RS11320 0.46 +1.0
2,447,881 + RS_RS11320 0.46 -0.7
2,447,882 - RS_RS11320 0.46 +0.8
2,447,882 - RS_RS11320 0.46 -0.1

Or see this region's nucleotide sequence