Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS10515 and RS_RS10520 are separated by 27 nucleotides RS_RS10520 and RS_RS10525 are separated by 5 nucleotides
RS_RS10515: RS_RS10515 - xanthine dehydrogenase molybdopterin binding subunit, at 2,268,893 to 2,271,271
_RS10515
RS_RS10520: RS_RS10520 - xanthine dehydrogenase accessory protein XdhC, at 2,271,299 to 2,272,327
_RS10520
RS_RS10525: RS_RS10525 - adenosine deaminase, at 2,272,333 to 2,273,370
_RS10525
Position (kb)
2271
2272
2273 Strain fitness (log2 ratio)
-2
-1
0
1 at 2270.330 kb on + strand, within RS_RS10515 at 2270.330 kb on + strand, within RS_RS10515 at 2270.331 kb on - strand, within RS_RS10515 at 2270.586 kb on - strand, within RS_RS10515 at 2270.691 kb on - strand, within RS_RS10515 at 2270.726 kb on + strand, within RS_RS10515 at 2270.726 kb on + strand, within RS_RS10515 at 2270.780 kb on + strand, within RS_RS10515 at 2270.783 kb on + strand, within RS_RS10515 at 2270.784 kb on - strand, within RS_RS10515 at 2270.784 kb on - strand, within RS_RS10515 at 2270.793 kb on - strand, within RS_RS10515 at 2271.552 kb on - strand, within RS_RS10520 at 2271.996 kb on - strand, within RS_RS10520 at 2272.073 kb on + strand, within RS_RS10520 at 2272.210 kb on + strand, within RS_RS10520 at 2272.326 kb on - strand at 2272.326 kb on - strand at 2272.466 kb on - strand, within RS_RS10525 at 2272.496 kb on - strand, within RS_RS10525 at 2272.531 kb on + strand, within RS_RS10525 at 2272.532 kb on - strand, within RS_RS10525 at 2272.535 kb on - strand, within RS_RS10525 at 2272.535 kb on - strand, within RS_RS10525 at 2272.591 kb on + strand, within RS_RS10525 at 2272.817 kb on - strand, within RS_RS10525 at 2272.817 kb on - strand, within RS_RS10525 at 2273.197 kb on + strand, within RS_RS10525 at 2273.209 kb on + strand, within RS_RS10525 at 2273.210 kb on - strand, within RS_RS10525
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 2,270,330 + RS_RS10515 0.60 +0.6 2,270,330 + RS_RS10515 0.60 -0.2 2,270,331 - RS_RS10515 0.60 -0.2 2,270,586 - RS_RS10515 0.71 -0.2 2,270,691 - RS_RS10515 0.76 -1.7 2,270,726 + RS_RS10515 0.77 -0.8 2,270,726 + RS_RS10515 0.77 -0.3 2,270,780 + RS_RS10515 0.79 -0.2 2,270,783 + RS_RS10515 0.79 -0.8 2,270,784 - RS_RS10515 0.79 +0.8 2,270,784 - RS_RS10515 0.79 +0.3 2,270,793 - RS_RS10515 0.80 +0.5 2,271,552 - RS_RS10520 0.25 +0.4 2,271,996 - RS_RS10520 0.68 -2.0 2,272,073 + RS_RS10520 0.75 +0.4 2,272,210 + RS_RS10520 0.89 +0.8 2,272,326 - -0.3 2,272,326 - +1.1 2,272,466 - RS_RS10525 0.13 +0.1 2,272,496 - RS_RS10525 0.16 -0.2 2,272,531 + RS_RS10525 0.19 -0.1 2,272,532 - RS_RS10525 0.19 +0.6 2,272,535 - RS_RS10525 0.19 +0.3 2,272,535 - RS_RS10525 0.19 +0.3 2,272,591 + RS_RS10525 0.25 +0.2 2,272,817 - RS_RS10525 0.47 -0.2 2,272,817 - RS_RS10525 0.47 -0.7 2,273,197 + RS_RS10525 0.83 -1.5 2,273,209 + RS_RS10525 0.84 +0.1 2,273,210 - RS_RS10525 0.84 +0.6
Or see this region's nucleotide sequence