Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS10380

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS10375 and RS_RS10380 are separated by 307 nucleotidesRS_RS10380 and RS_RS10385 are separated by 161 nucleotides RS_RS10375: RS_RS10375 - polyribonucleotide nucleotidyltransferase, at 2,239,684 to 2,241,837 _RS10375 RS_RS10380: RS_RS10380 - 30S ribosomal protein S15, at 2,242,145 to 2,242,414 _RS10380 RS_RS10385: RS_RS10385 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,242,576 to 2,243,832 _RS10385 Position (kb) 2242 2243Strain fitness (log2 ratio) -1 0 1at 2242.581 kb on - strandat 2243.039 kb on + strand, within RS_RS10385at 2243.039 kb on + strand, within RS_RS10385at 2243.040 kb on - strand, within RS_RS10385at 2243.163 kb on - strand, within RS_RS10385at 2243.282 kb on + strand, within RS_RS10385at 2243.282 kb on + strand, within RS_RS10385at 2243.283 kb on - strand, within RS_RS10385at 2243.301 kb on - strand, within RS_RS10385

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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2,242,581 - +0.8
2,243,039 + RS_RS10385 0.37 +0.8
2,243,039 + RS_RS10385 0.37 +0.1
2,243,040 - RS_RS10385 0.37 -1.1
2,243,163 - RS_RS10385 0.47 -0.9
2,243,282 + RS_RS10385 0.56 +0.4
2,243,282 + RS_RS10385 0.56 +0.1
2,243,283 - RS_RS10385 0.56 -0.5
2,243,301 - RS_RS10385 0.58 +0.1

Or see this region's nucleotide sequence