Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS10370

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS10365 and RS_RS10370 are separated by 17 nucleotidesRS_RS10370 and RS_RS10375 are separated by 91 nucleotides RS_RS10365: RS_RS10365 - NAD(P)H quinone oxidoreductase, at 2,237,620 to 2,238,624 _RS10365 RS_RS10370: RS_RS10370 - haloacid dehalogenase-like hydrolase, at 2,238,642 to 2,239,592 _RS10370 RS_RS10375: RS_RS10375 - polyribonucleotide nucleotidyltransferase, at 2,239,684 to 2,241,837 _RS10375 Position (kb) 2238 2239 2240Strain fitness (log2 ratio) -2 -1 0 1at 2237.942 kb on + strand, within RS_RS10365at 2237.942 kb on + strand, within RS_RS10365at 2237.943 kb on - strand, within RS_RS10365at 2238.599 kb on + strandat 2238.600 kb on - strandat 2238.643 kb on + strandat 2238.644 kb on - strandat 2238.889 kb on + strand, within RS_RS10370at 2238.889 kb on + strand, within RS_RS10370at 2238.956 kb on - strand, within RS_RS10370at 2238.956 kb on - strand, within RS_RS10370at 2239.087 kb on + strand, within RS_RS10370at 2239.087 kb on + strand, within RS_RS10370at 2239.220 kb on - strand, within RS_RS10370at 2239.220 kb on - strand, within RS_RS10370at 2239.220 kb on - strand, within RS_RS10370at 2239.523 kb on - strandat 2239.685 kb on + strandat 2239.964 kb on + strand, within RS_RS10375at 2239.965 kb on - strand, within RS_RS10375at 2239.965 kb on - strand, within RS_RS10375

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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2,237,942 + RS_RS10365 0.32 +0.7
2,237,942 + RS_RS10365 0.32 +0.5
2,237,943 - RS_RS10365 0.32 +0.7
2,238,599 + -0.8
2,238,600 - +0.8
2,238,643 + -0.6
2,238,644 - +0.2
2,238,889 + RS_RS10370 0.26 -0.1
2,238,889 + RS_RS10370 0.26 -0.5
2,238,956 - RS_RS10370 0.33 -0.4
2,238,956 - RS_RS10370 0.33 +0.2
2,239,087 + RS_RS10370 0.47 +0.5
2,239,087 + RS_RS10370 0.47 +0.5
2,239,220 - RS_RS10370 0.61 -0.4
2,239,220 - RS_RS10370 0.61 -0.2
2,239,220 - RS_RS10370 0.61 +0.1
2,239,523 - -0.2
2,239,685 + +0.8
2,239,964 + RS_RS10375 0.13 -2.0
2,239,965 - RS_RS10375 0.13 +0.8
2,239,965 - RS_RS10375 0.13 -2.3

Or see this region's nucleotide sequence