Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS01335 and RS_RS01340 are separated by 24 nucleotides RS_RS01340 and RS_RS01345 are separated by 43 nucleotides RS_RS01345 and RS_RS01350 are separated by 10 nucleotides RS_RS01350 and RS_RS01355 are separated by 10 nucleotides
RS_RS01335: RS_RS01335 - rhodanese-like domain-containing protein, at 304,840 to 305,265
_RS01335
RS_RS01340: RS_RS01340 - 2-hydroxychromene-2-carboxylate isomerase, at 305,290 to 305,895
_RS01340
RS_RS01345: RS_RS01345 - butyryl-CoA dehydrogenase, at 305,939 to 307,069
_RS01345
RS_RS01350: RS_RS01350 - short chain dehydrogenase, at 307,080 to 307,790
_RS01350
RS_RS01355: RS_RS01355 - acetyl-CoA C-acyltransferase, at 307,801 to 308,982
_RS01355
Position (kb) 305
306
307
308 Strain fitness (log2 ratio) -1
0
1
2 at 305.288 kb on + strand at 305.289 kb on - strand at 305.291 kb on + strand at 305.291 kb on + strand at 305.291 kb on + strand at 305.292 kb on - strand at 305.292 kb on - strand at 305.292 kb on - strand at 305.292 kb on - strand at 305.627 kb on + strand, within RS_RS01340 at 305.807 kb on + strand, within RS_RS01340 at 305.807 kb on + strand, within RS_RS01340 at 305.808 kb on - strand, within RS_RS01340 at 305.808 kb on - strand, within RS_RS01340 at 305.808 kb on - strand, within RS_RS01340 at 305.873 kb on + strand at 305.991 kb on + strand at 305.991 kb on + strand at 305.991 kb on + strand at 305.991 kb on + strand at 305.991 kb on + strand at 305.992 kb on - strand at 305.992 kb on - strand at 306.286 kb on - strand, within RS_RS01345 at 306.498 kb on + strand, within RS_RS01345 at 306.498 kb on + strand, within RS_RS01345 at 306.880 kb on - strand, within RS_RS01345 at 306.880 kb on - strand, within RS_RS01345 at 306.880 kb on - strand, within RS_RS01345 at 306.880 kb on - strand, within RS_RS01345 at 307.528 kb on + strand, within RS_RS01350 at 307.529 kb on - strand, within RS_RS01350 at 307.529 kb on - strand, within RS_RS01350 at 307.544 kb on - strand, within RS_RS01350 at 307.708 kb on + strand, within RS_RS01350 at 307.708 kb on + strand, within RS_RS01350 at 307.708 kb on + strand, within RS_RS01350 at 307.709 kb on - strand, within RS_RS01350
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 305,288 + -0.9 305,289 - +0.6 305,291 + -0.0 305,291 + -1.2 305,291 + -0.7 305,292 - -0.2 305,292 - +1.1 305,292 - +2.7 305,292 - +0.5 305,627 + RS_RS01340 0.56 +0.0 305,807 + RS_RS01340 0.85 -0.5 305,807 + RS_RS01340 0.85 -0.6 305,808 - RS_RS01340 0.85 +0.5 305,808 - RS_RS01340 0.85 +0.2 305,808 - RS_RS01340 0.85 -0.2 305,873 + -0.5 305,991 + -0.2 305,991 + -0.8 305,991 + +0.6 305,991 + -0.1 305,991 + +1.1 305,992 - +1.1 305,992 - +0.4 306,286 - RS_RS01345 0.31 -0.2 306,498 + RS_RS01345 0.49 +1.1 306,498 + RS_RS01345 0.49 -0.4 306,880 - RS_RS01345 0.83 +1.0 306,880 - RS_RS01345 0.83 +1.0 306,880 - RS_RS01345 0.83 -0.8 306,880 - RS_RS01345 0.83 +0.5 307,528 + RS_RS01350 0.63 -0.6 307,529 - RS_RS01350 0.63 +0.5 307,529 - RS_RS01350 0.63 +1.0 307,544 - RS_RS01350 0.65 +0.4 307,708 + RS_RS01350 0.88 +0.1 307,708 + RS_RS01350 0.88 +0.5 307,708 + RS_RS01350 0.88 +0.1 307,709 - RS_RS01350 0.88 +0.2
Or see this region's nucleotide sequence