Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS00650 and RS_RS00655 are separated by 309 nucleotides RS_RS00655 and RS_RS00660 are separated by 72 nucleotides RS_RS00660 and RS_RS00665 are separated by 240 nucleotides
RS_RS00650: RS_RS00650 - AraC family transcriptional regulator, at 154,813 to 155,694
_RS00650
RS_RS00655: RS_RS00655 - GYD domain-containing protein, at 156,004 to 156,297
_RS00655
RS_RS00660: RS_RS00660 - S-adenosylmethionine synthase, at 156,370 to 157,560
_RS00660
RS_RS00665: RS_RS00665 - lauroyl acyltransferase, at 157,801 to 158,649
_RS00665
Position (kb) 156
157
158 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 155.522 kb on + strand, within RS_RS00650 at 155.522 kb on + strand, within RS_RS00650 at 155.522 kb on + strand, within RS_RS00650 at 155.522 kb on + strand, within RS_RS00650 at 155.522 kb on + strand, within RS_RS00650 at 155.522 kb on + strand, within RS_RS00650 at 155.523 kb on - strand, within RS_RS00650 at 155.523 kb on - strand, within RS_RS00650 at 155.523 kb on - strand, within RS_RS00650 at 155.523 kb on - strand, within RS_RS00650 at 155.523 kb on - strand, within RS_RS00650 at 155.673 kb on - strand at 155.970 kb on + strand at 156.148 kb on + strand, within RS_RS00655 at 156.148 kb on + strand, within RS_RS00655 at 156.148 kb on + strand, within RS_RS00655 at 156.319 kb on + strand at 156.319 kb on + strand at 157.635 kb on - strand at 157.635 kb on - strand at 157.635 kb on - strand at 157.713 kb on + strand at 157.713 kb on + strand at 157.713 kb on + strand at 158.087 kb on + strand, within RS_RS00665 at 158.088 kb on - strand, within RS_RS00665 at 158.155 kb on + strand, within RS_RS00665 at 158.318 kb on + strand, within RS_RS00665 at 158.390 kb on - strand, within RS_RS00665 at 158.391 kb on + strand, within RS_RS00665 at 158.391 kb on + strand, within RS_RS00665 at 158.392 kb on - strand, within RS_RS00665 at 158.392 kb on - strand, within RS_RS00665 at 158.486 kb on + strand, within RS_RS00665 at 158.486 kb on + strand, within RS_RS00665
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 155,522 + RS_RS00650 0.80 +0.2 155,522 + RS_RS00650 0.80 +0.4 155,522 + RS_RS00650 0.80 -0.0 155,522 + RS_RS00650 0.80 +0.8 155,522 + RS_RS00650 0.80 +0.3 155,522 + RS_RS00650 0.80 +0.2 155,523 - RS_RS00650 0.80 +1.5 155,523 - RS_RS00650 0.80 -0.4 155,523 - RS_RS00650 0.80 +0.8 155,523 - RS_RS00650 0.80 +0.2 155,523 - RS_RS00650 0.80 -0.1 155,673 - +0.2 155,970 + -0.1 156,148 + RS_RS00655 0.49 +0.3 156,148 + RS_RS00655 0.49 -0.6 156,148 + RS_RS00655 0.49 +0.6 156,319 + -0.5 156,319 + -0.3 157,635 - -0.2 157,635 - +1.2 157,635 - +0.2 157,713 + -0.8 157,713 + -1.0 157,713 + -0.2 158,087 + RS_RS00665 0.34 -5.1 158,088 - RS_RS00665 0.34 -2.0 158,155 + RS_RS00665 0.42 -5.3 158,318 + RS_RS00665 0.61 -5.1 158,390 - RS_RS00665 0.69 -5.6 158,391 + RS_RS00665 0.69 -3.2 158,391 + RS_RS00665 0.69 -5.4 158,392 - RS_RS00665 0.70 -4.9 158,392 - RS_RS00665 0.70 -5.5 158,486 + RS_RS00665 0.81 -5.6 158,486 + RS_RS00665 0.81 -4.9
Or see this region's nucleotide sequence