Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS16695 and RS_RS16700 are separated by 232 nucleotides RS_RS16700 and RS_RS16705 are separated by 9 nucleotides RS_RS16705 and RS_RS16710 are separated by 100 nucleotides
RS_RS16695: RS_RS16695 - LysR family transcriptional regulator, at 3,594,672 to 3,595,568
_RS16695
RS_RS16700: RS_RS16700 - hypothetical protein, at 3,595,801 to 3,596,016
_RS16700
RS_RS16705: RS_RS16705 - LysR family transcriptional regulator, at 3,596,026 to 3,596,922
_RS16705
RS_RS16710: RS_RS16710 - membrane protein, at 3,597,023 to 3,597,979
_RS16710
Position (kb)
3596
3597 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3595.182 kb on + strand, within RS_RS16695 at 3595.183 kb on - strand, within RS_RS16695 at 3595.449 kb on + strand, within RS_RS16695 at 3595.449 kb on + strand, within RS_RS16695 at 3595.450 kb on - strand, within RS_RS16695 at 3595.450 kb on - strand, within RS_RS16695 at 3595.609 kb on - strand at 3595.609 kb on - strand at 3595.636 kb on - strand at 3595.636 kb on - strand at 3595.655 kb on + strand at 3595.655 kb on + strand at 3595.656 kb on - strand at 3595.669 kb on + strand at 3595.670 kb on - strand at 3595.855 kb on + strand, within RS_RS16700 at 3595.856 kb on - strand, within RS_RS16700 at 3595.856 kb on - strand, within RS_RS16700 at 3595.891 kb on + strand, within RS_RS16700 at 3595.891 kb on + strand, within RS_RS16700 at 3595.891 kb on + strand, within RS_RS16700 at 3596.043 kb on - strand at 3596.205 kb on + strand, within RS_RS16705 at 3596.205 kb on + strand, within RS_RS16705 at 3596.205 kb on + strand, within RS_RS16705 at 3596.206 kb on - strand, within RS_RS16705 at 3596.206 kb on - strand, within RS_RS16705 at 3596.336 kb on + strand, within RS_RS16705 at 3596.399 kb on + strand, within RS_RS16705 at 3596.559 kb on - strand, within RS_RS16705 at 3596.932 kb on + strand at 3596.972 kb on - strand at 3596.972 kb on - strand at 3596.972 kb on - strand at 3597.359 kb on + strand, within RS_RS16710 at 3597.580 kb on + strand, within RS_RS16710 at 3597.626 kb on + strand, within RS_RS16710 at 3597.626 kb on + strand, within RS_RS16710 at 3597.626 kb on + strand, within RS_RS16710 at 3597.626 kb on + strand, within RS_RS16710 at 3597.627 kb on - strand, within RS_RS16710 at 3597.627 kb on - strand, within RS_RS16710 at 3597.627 kb on - strand, within RS_RS16710 at 3597.627 kb on - strand, within RS_RS16710 at 3597.778 kb on + strand, within RS_RS16710 at 3597.857 kb on + strand, within RS_RS16710 at 3597.858 kb on - strand, within RS_RS16710
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 3,595,182 + RS_RS16695 0.57 -0.5 3,595,183 - RS_RS16695 0.57 +1.5 3,595,449 + RS_RS16695 0.87 -0.3 3,595,449 + RS_RS16695 0.87 +2.9 3,595,450 - RS_RS16695 0.87 +0.0 3,595,450 - RS_RS16695 0.87 +0.3 3,595,609 - -1.3 3,595,609 - -0.5 3,595,636 - +0.2 3,595,636 - +0.1 3,595,655 + +0.5 3,595,655 + +1.5 3,595,656 - +1.0 3,595,669 + -0.8 3,595,670 - -0.8 3,595,855 + RS_RS16700 0.25 -1.8 3,595,856 - RS_RS16700 0.25 -0.6 3,595,856 - RS_RS16700 0.25 -0.8 3,595,891 + RS_RS16700 0.42 -0.9 3,595,891 + RS_RS16700 0.42 -1.1 3,595,891 + RS_RS16700 0.42 +0.7 3,596,043 - +0.0 3,596,205 + RS_RS16705 0.20 -0.4 3,596,205 + RS_RS16705 0.20 -0.1 3,596,205 + RS_RS16705 0.20 -1.2 3,596,206 - RS_RS16705 0.20 -0.7 3,596,206 - RS_RS16705 0.20 +0.3 3,596,336 + RS_RS16705 0.35 -0.2 3,596,399 + RS_RS16705 0.42 +1.5 3,596,559 - RS_RS16705 0.59 +0.1 3,596,932 + -0.0 3,596,972 - -0.1 3,596,972 - -1.3 3,596,972 - -0.3 3,597,359 + RS_RS16710 0.35 +0.1 3,597,580 + RS_RS16710 0.58 -1.0 3,597,626 + RS_RS16710 0.63 +0.5 3,597,626 + RS_RS16710 0.63 -0.4 3,597,626 + RS_RS16710 0.63 -0.6 3,597,626 + RS_RS16710 0.63 +1.0 3,597,627 - RS_RS16710 0.63 -0.1 3,597,627 - RS_RS16710 0.63 +2.5 3,597,627 - RS_RS16710 0.63 -0.2 3,597,627 - RS_RS16710 0.63 -0.1 3,597,778 + RS_RS16710 0.79 -0.3 3,597,857 + RS_RS16710 0.87 -0.1 3,597,858 - RS_RS16710 0.87 +0.5
Or see this region's nucleotide sequence