Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS14735

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS14725 and RS_RS14730 are separated by 83 nucleotidesRS_RS14730 and RS_RS14735 are separated by 56 nucleotidesRS_RS14735 and RS_RS14740 are separated by 21 nucleotidesRS_RS14740 and RS_RS14745 are separated by 61 nucleotidesRS_RS14745 and RS_RS14750 are separated by 46 nucleotides RS_RS14725: RS_RS14725 - ABC transporter ATP-binding protein, at 3,171,658 to 3,172,734 _RS14725 RS_RS14730: RS_RS14730 - twin-arginine translocase subunit TatC, at 3,172,818 to 3,173,621 _RS14730 RS_RS14735: RS_RS14735 - twin-arginine translocase subunit TatB, at 3,173,678 to 3,174,196 _RS14735 RS_RS14740: RS_RS14740 - twin-arginine translocase subunit TatA, at 3,174,218 to 3,174,475 _RS14740 RS_RS14745: RS_RS14745 - membrane protein, at 3,174,537 to 3,174,911 _RS14745 RS_RS14750: RS_RS14750 - phosphoribosyl-ATP pyrophosphatase, at 3,174,958 to 3,175,332 _RS14750 Position (kb) 3173 3174 3175Strain fitness (log2 ratio) -1 0 1at 3172.909 kb on + strand, within RS_RS14730at 3172.910 kb on - strand, within RS_RS14730at 3173.125 kb on + strand, within RS_RS14730at 3173.126 kb on - strand, within RS_RS14730at 3173.170 kb on + strand, within RS_RS14730at 3173.171 kb on - strand, within RS_RS14730at 3173.246 kb on - strand, within RS_RS14730at 3173.790 kb on + strand, within RS_RS14735at 3174.073 kb on - strand, within RS_RS14735at 3174.490 kb on + strandat 3174.551 kb on - strandat 3174.594 kb on - strand, within RS_RS14745at 3174.594 kb on - strand, within RS_RS14745at 3174.594 kb on - strand, within RS_RS14745at 3174.684 kb on + strand, within RS_RS14745at 3174.684 kb on + strand, within RS_RS14745at 3174.901 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
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3,172,909 + RS_RS14730 0.11 -0.1
3,172,910 - RS_RS14730 0.11 -0.1
3,173,125 + RS_RS14730 0.38 +1.5
3,173,126 - RS_RS14730 0.38 +1.5
3,173,170 + RS_RS14730 0.44 -1.6
3,173,171 - RS_RS14730 0.44 +1.5
3,173,246 - RS_RS14730 0.53 +0.5
3,173,790 + RS_RS14735 0.22 +1.5
3,174,073 - RS_RS14735 0.76 +1.5
3,174,490 + +1.5
3,174,551 - +0.2
3,174,594 - RS_RS14745 0.15 -0.3
3,174,594 - RS_RS14745 0.15 -0.0
3,174,594 - RS_RS14745 0.15 -0.1
3,174,684 + RS_RS14745 0.39 -0.6
3,174,684 + RS_RS14745 0.39 +1.5
3,174,901 + +1.3

Or see this region's nucleotide sequence