Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS13520 and RS_RS25600 are separated by 86 nucleotides RS_RS25600 and RS_RS13525 are separated by 93 nucleotides RS_RS13525 and RS_RS25605 are separated by 445 nucleotides
RS_RS13520: RS_RS13520 - hypothetical protein, at 2,907,429 to 2,907,803
_RS13520
RS_RS25600: RS_RS25600 - hypothetical protein, at 2,907,890 to 2,908,174
_RS25600
RS_RS13525: RS_RS13525 - hypothetical protein, at 2,908,268 to 2,908,594
_RS13525
RS_RS25605: RS_RS25605 - cold-shock protein, at 2,909,040 to 2,909,204
_RS25605
Position (kb)
2908
2909 Strain fitness (log2 ratio)
-1
0
1 at 2907.525 kb on - strand, within RS_RS13520 at 2907.546 kb on - strand, within RS_RS13520 at 2907.551 kb on - strand, within RS_RS13520 at 2907.606 kb on - strand, within RS_RS13520 at 2907.737 kb on - strand, within RS_RS13520 at 2907.743 kb on + strand, within RS_RS13520 at 2908.119 kb on + strand, within RS_RS25600 at 2908.120 kb on - strand, within RS_RS25600 at 2908.120 kb on - strand, within RS_RS25600 at 2908.132 kb on - strand, within RS_RS25600 at 2908.149 kb on + strand at 2908.149 kb on + strand at 2908.149 kb on + strand at 2908.149 kb on + strand at 2908.194 kb on + strand at 2908.195 kb on - strand at 2908.195 kb on - strand at 2908.195 kb on - strand at 2908.199 kb on - strand at 2908.231 kb on + strand at 2908.231 kb on + strand at 2908.232 kb on - strand at 2908.269 kb on + strand at 2908.269 kb on + strand at 2908.270 kb on - strand at 2908.270 kb on - strand at 2908.355 kb on + strand, within RS_RS13525 at 2908.388 kb on + strand, within RS_RS13525 at 2908.388 kb on + strand, within RS_RS13525 at 2908.388 kb on + strand, within RS_RS13525 at 2908.388 kb on + strand, within RS_RS13525 at 2908.389 kb on - strand, within RS_RS13525 at 2908.389 kb on - strand, within RS_RS13525 at 2908.389 kb on - strand, within RS_RS13525 at 2908.487 kb on + strand, within RS_RS13525 at 2908.488 kb on - strand, within RS_RS13525 at 2908.488 kb on - strand, within RS_RS13525 at 2908.521 kb on - strand, within RS_RS13525 at 2908.909 kb on + strand at 2908.931 kb on - strand at 2908.946 kb on - strand at 2909.095 kb on + strand, within RS_RS25605 at 2909.095 kb on + strand, within RS_RS25605 at 2909.095 kb on + strand, within RS_RS25605 at 2909.095 kb on + strand, within RS_RS25605 at 2909.095 kb on + strand, within RS_RS25605 at 2909.096 kb on - strand, within RS_RS25605 at 2909.096 kb on - strand, within RS_RS25605 at 2909.222 kb on + strand at 2909.435 kb on + strand at 2909.435 kb on + strand at 2909.435 kb on + strand at 2909.435 kb on + strand at 2909.478 kb on - strand at 2909.542 kb on + strand at 2909.543 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 2,907,525 - RS_RS13520 0.26 +0.2 2,907,546 - RS_RS13520 0.31 +0.2 2,907,551 - RS_RS13520 0.33 +0.4 2,907,606 - RS_RS13520 0.47 -0.1 2,907,737 - RS_RS13520 0.82 +0.8 2,907,743 + RS_RS13520 0.84 -0.1 2,908,119 + RS_RS25600 0.80 +0.0 2,908,120 - RS_RS25600 0.81 -0.3 2,908,120 - RS_RS25600 0.81 -0.0 2,908,132 - RS_RS25600 0.85 +1.4 2,908,149 + -0.2 2,908,149 + +0.4 2,908,149 + +0.4 2,908,149 + -0.3 2,908,194 + +0.2 2,908,195 - -0.2 2,908,195 - -0.0 2,908,195 - -0.0 2,908,199 - +0.0 2,908,231 + +1.4 2,908,231 + -0.8 2,908,232 - +0.9 2,908,269 + +0.2 2,908,269 + +0.3 2,908,270 - +0.1 2,908,270 - -0.5 2,908,355 + RS_RS13525 0.27 -0.1 2,908,388 + RS_RS13525 0.37 +0.5 2,908,388 + RS_RS13525 0.37 +0.7 2,908,388 + RS_RS13525 0.37 -0.1 2,908,388 + RS_RS13525 0.37 +0.4 2,908,389 - RS_RS13525 0.37 +1.4 2,908,389 - RS_RS13525 0.37 -0.4 2,908,389 - RS_RS13525 0.37 +0.3 2,908,487 + RS_RS13525 0.67 +0.3 2,908,488 - RS_RS13525 0.67 +0.1 2,908,488 - RS_RS13525 0.67 -0.4 2,908,521 - RS_RS13525 0.77 -0.2 2,908,909 + -0.0 2,908,931 - -0.3 2,908,946 - -0.1 2,909,095 + RS_RS25605 0.33 -0.5 2,909,095 + RS_RS25605 0.33 +0.0 2,909,095 + RS_RS25605 0.33 -1.2 2,909,095 + RS_RS25605 0.33 -0.4 2,909,095 + RS_RS25605 0.33 -0.8 2,909,096 - RS_RS25605 0.34 -0.3 2,909,096 - RS_RS25605 0.34 -0.1 2,909,222 + +0.2 2,909,435 + -0.8 2,909,435 + -0.4 2,909,435 + +0.0 2,909,435 + +0.4 2,909,478 - +0.9 2,909,542 + +0.2 2,909,543 - -0.2
Or see this region's nucleotide sequence