Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS13525

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS13520 and RS_RS25600 are separated by 86 nucleotidesRS_RS25600 and RS_RS13525 are separated by 93 nucleotidesRS_RS13525 and RS_RS25605 are separated by 445 nucleotides RS_RS13520: RS_RS13520 - hypothetical protein, at 2,907,429 to 2,907,803 _RS13520 RS_RS25600: RS_RS25600 - hypothetical protein, at 2,907,890 to 2,908,174 _RS25600 RS_RS13525: RS_RS13525 - hypothetical protein, at 2,908,268 to 2,908,594 _RS13525 RS_RS25605: RS_RS25605 - cold-shock protein, at 2,909,040 to 2,909,204 _RS25605 Position (kb) 2908 2909Strain fitness (log2 ratio) -1 0 1at 2907.525 kb on - strand, within RS_RS13520at 2907.546 kb on - strand, within RS_RS13520at 2907.551 kb on - strand, within RS_RS13520at 2907.606 kb on - strand, within RS_RS13520at 2907.737 kb on - strand, within RS_RS13520at 2907.743 kb on + strand, within RS_RS13520at 2908.119 kb on + strand, within RS_RS25600at 2908.120 kb on - strand, within RS_RS25600at 2908.120 kb on - strand, within RS_RS25600at 2908.132 kb on - strand, within RS_RS25600at 2908.149 kb on + strandat 2908.149 kb on + strandat 2908.149 kb on + strandat 2908.149 kb on + strandat 2908.194 kb on + strandat 2908.195 kb on - strandat 2908.195 kb on - strandat 2908.195 kb on - strandat 2908.199 kb on - strandat 2908.231 kb on + strandat 2908.231 kb on + strandat 2908.232 kb on - strandat 2908.269 kb on + strandat 2908.269 kb on + strandat 2908.270 kb on - strandat 2908.270 kb on - strandat 2908.355 kb on + strand, within RS_RS13525at 2908.388 kb on + strand, within RS_RS13525at 2908.388 kb on + strand, within RS_RS13525at 2908.388 kb on + strand, within RS_RS13525at 2908.388 kb on + strand, within RS_RS13525at 2908.389 kb on - strand, within RS_RS13525at 2908.389 kb on - strand, within RS_RS13525at 2908.389 kb on - strand, within RS_RS13525at 2908.487 kb on + strand, within RS_RS13525at 2908.488 kb on - strand, within RS_RS13525at 2908.488 kb on - strand, within RS_RS13525at 2908.521 kb on - strand, within RS_RS13525at 2908.909 kb on + strandat 2908.931 kb on - strandat 2908.946 kb on - strandat 2909.095 kb on + strand, within RS_RS25605at 2909.095 kb on + strand, within RS_RS25605at 2909.095 kb on + strand, within RS_RS25605at 2909.095 kb on + strand, within RS_RS25605at 2909.095 kb on + strand, within RS_RS25605at 2909.096 kb on - strand, within RS_RS25605at 2909.096 kb on - strand, within RS_RS25605at 2909.222 kb on + strandat 2909.435 kb on + strandat 2909.435 kb on + strandat 2909.435 kb on + strandat 2909.435 kb on + strandat 2909.478 kb on - strandat 2909.542 kb on + strandat 2909.543 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
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2,907,525 - RS_RS13520 0.26 +0.2
2,907,546 - RS_RS13520 0.31 +0.2
2,907,551 - RS_RS13520 0.33 +0.4
2,907,606 - RS_RS13520 0.47 -0.1
2,907,737 - RS_RS13520 0.82 +0.8
2,907,743 + RS_RS13520 0.84 -0.1
2,908,119 + RS_RS25600 0.80 +0.0
2,908,120 - RS_RS25600 0.81 -0.3
2,908,120 - RS_RS25600 0.81 -0.0
2,908,132 - RS_RS25600 0.85 +1.4
2,908,149 + -0.2
2,908,149 + +0.4
2,908,149 + +0.4
2,908,149 + -0.3
2,908,194 + +0.2
2,908,195 - -0.2
2,908,195 - -0.0
2,908,195 - -0.0
2,908,199 - +0.0
2,908,231 + +1.4
2,908,231 + -0.8
2,908,232 - +0.9
2,908,269 + +0.2
2,908,269 + +0.3
2,908,270 - +0.1
2,908,270 - -0.5
2,908,355 + RS_RS13525 0.27 -0.1
2,908,388 + RS_RS13525 0.37 +0.5
2,908,388 + RS_RS13525 0.37 +0.7
2,908,388 + RS_RS13525 0.37 -0.1
2,908,388 + RS_RS13525 0.37 +0.4
2,908,389 - RS_RS13525 0.37 +1.4
2,908,389 - RS_RS13525 0.37 -0.4
2,908,389 - RS_RS13525 0.37 +0.3
2,908,487 + RS_RS13525 0.67 +0.3
2,908,488 - RS_RS13525 0.67 +0.1
2,908,488 - RS_RS13525 0.67 -0.4
2,908,521 - RS_RS13525 0.77 -0.2
2,908,909 + -0.0
2,908,931 - -0.3
2,908,946 - -0.1
2,909,095 + RS_RS25605 0.33 -0.5
2,909,095 + RS_RS25605 0.33 +0.0
2,909,095 + RS_RS25605 0.33 -1.2
2,909,095 + RS_RS25605 0.33 -0.4
2,909,095 + RS_RS25605 0.33 -0.8
2,909,096 - RS_RS25605 0.34 -0.3
2,909,096 - RS_RS25605 0.34 -0.1
2,909,222 + +0.2
2,909,435 + -0.8
2,909,435 + -0.4
2,909,435 + +0.0
2,909,435 + +0.4
2,909,478 - +0.9
2,909,542 + +0.2
2,909,543 - -0.2

Or see this region's nucleotide sequence