Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS13210

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS13205 and RS_RS13210 are separated by 363 nucleotidesRS_RS13210 and RS_RS13215 are separated by 263 nucleotidesRS_RS13215 and RS_RS13220 are separated by 15 nucleotides RS_RS13205: RS_RS13205 - molecular chaperone DnaK, at 2,838,006 to 2,839,964 _RS13205 RS_RS13210: RS_RS13210 - nucleoside diphosphate kinase regulator, at 2,840,328 to 2,840,732 _RS13210 RS_RS13215: RS_RS13215 - thioredoxin, at 2,840,996 to 2,841,418 _RS13215 RS_RS13220: RS_RS13220 - nucleotide exchange factor GrpE, at 2,841,434 to 2,842,078 _RS13220 Position (kb) 2840 2841Strain fitness (log2 ratio) -1 0 1at 2840.005 kb on - strandat 2840.235 kb on + strandat 2840.338 kb on + strandat 2840.339 kb on - strandat 2840.339 kb on - strandat 2840.393 kb on + strand, within RS_RS13210at 2840.393 kb on + strand, within RS_RS13210at 2840.413 kb on + strand, within RS_RS13210at 2840.414 kb on - strand, within RS_RS13210at 2840.414 kb on - strand, within RS_RS13210at 2840.713 kb on + strandat 2840.713 kb on + strandat 2840.713 kb on + strandat 2840.714 kb on - strandat 2840.714 kb on - strandat 2841.436 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
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2,840,005 - +0.1
2,840,235 + +0.2
2,840,338 + -0.4
2,840,339 - -0.4
2,840,339 - +0.2
2,840,393 + RS_RS13210 0.16 -0.5
2,840,393 + RS_RS13210 0.16 +0.3
2,840,413 + RS_RS13210 0.21 -0.3
2,840,414 - RS_RS13210 0.21 -0.5
2,840,414 - RS_RS13210 0.21 -0.1
2,840,713 + +0.0
2,840,713 + +0.0
2,840,713 + +0.4
2,840,714 - +0.4
2,840,714 - -0.9
2,841,436 - +0.3

Or see this region's nucleotide sequence