Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS10975 and RS_RS10980 are separated by 147 nucleotides RS_RS10980 and RS_RS10985 are separated by 91 nucleotides
RS_RS10975: RS_RS10975 - EamA/RhaT family transporter, at 2,368,995 to 2,369,921
_RS10975
RS_RS10980: RS_RS10980 - uracil phosphoribosyltransferase, at 2,370,069 to 2,370,719
_RS10980
RS_RS10985: RS_RS10985 - ATP-binding protein, at 2,370,811 to 2,372,418
_RS10985
Position (kb)
2370
2371 Strain fitness (log2 ratio)
-2
-1
0
1 at 2369.237 kb on - strand, within RS_RS10975 at 2369.380 kb on + strand, within RS_RS10975 at 2369.380 kb on + strand, within RS_RS10975 at 2369.389 kb on + strand, within RS_RS10975 at 2369.390 kb on - strand, within RS_RS10975 at 2369.451 kb on + strand, within RS_RS10975 at 2369.451 kb on + strand, within RS_RS10975 at 2369.608 kb on + strand, within RS_RS10975 at 2369.644 kb on + strand, within RS_RS10975 at 2369.644 kb on + strand, within RS_RS10975 at 2369.645 kb on - strand, within RS_RS10975 at 2369.645 kb on - strand, within RS_RS10975 at 2369.800 kb on + strand, within RS_RS10975 at 2369.800 kb on + strand, within RS_RS10975 at 2369.801 kb on - strand, within RS_RS10975 at 2369.801 kb on - strand, within RS_RS10975 at 2369.970 kb on + strand at 2369.970 kb on + strand at 2369.971 kb on - strand at 2370.383 kb on + strand, within RS_RS10980 at 2370.663 kb on + strand at 2370.663 kb on + strand at 2370.663 kb on + strand at 2370.664 kb on - strand at 2370.664 kb on - strand at 2371.170 kb on - strand, within RS_RS10985 at 2371.529 kb on + strand, within RS_RS10985 at 2371.529 kb on + strand, within RS_RS10985 at 2371.530 kb on - strand, within RS_RS10985 at 2371.719 kb on - strand, within RS_RS10985 at 2371.719 kb on - strand, within RS_RS10985 at 2371.719 kb on - strand, within RS_RS10985
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 2,369,237 - RS_RS10975 0.26 -0.2 2,369,380 + RS_RS10975 0.42 -0.6 2,369,380 + RS_RS10975 0.42 -0.2 2,369,389 + RS_RS10975 0.43 +1.2 2,369,390 - RS_RS10975 0.43 +0.2 2,369,451 + RS_RS10975 0.49 +0.2 2,369,451 + RS_RS10975 0.49 +0.2 2,369,608 + RS_RS10975 0.66 +0.4 2,369,644 + RS_RS10975 0.70 -0.3 2,369,644 + RS_RS10975 0.70 -0.1 2,369,645 - RS_RS10975 0.70 -0.6 2,369,645 - RS_RS10975 0.70 -0.1 2,369,800 + RS_RS10975 0.87 +1.2 2,369,800 + RS_RS10975 0.87 -2.2 2,369,801 - RS_RS10975 0.87 +1.2 2,369,801 - RS_RS10975 0.87 -0.1 2,369,970 + +0.3 2,369,970 + +0.3 2,369,971 - -0.6 2,370,383 + RS_RS10980 0.48 -0.2 2,370,663 + +0.3 2,370,663 + +0.1 2,370,663 + -0.1 2,370,664 - -0.0 2,370,664 - -0.5 2,371,170 - RS_RS10985 0.22 +0.8 2,371,529 + RS_RS10985 0.45 +0.3 2,371,529 + RS_RS10985 0.45 +1.3 2,371,530 - RS_RS10985 0.45 +0.6 2,371,719 - RS_RS10985 0.56 +0.4 2,371,719 - RS_RS10985 0.56 +0.2 2,371,719 - RS_RS10985 0.56 +0.0
Or see this region's nucleotide sequence