Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS08805 and RS_RS08810 are separated by 836 nucleotides RS_RS08810 and RS_RS08815 are separated by 41 nucleotides RS_RS08815 and RS_RS08820 overlap by 1 nucleotides RS_RS08820 and RS_RS08825 are separated by 22 nucleotides
RS_RS08805: RS_RS08805 - hypothetical protein, at 1,865,631 to 1,865,873
_RS08805
RS_RS08810: RS_RS08810 - peptidyl-prolyl cis-trans isomerase, at 1,866,710 to 1,867,507
_RS08810
RS_RS08815: RS_RS08815 - BolA family transcriptional regulator, at 1,867,549 to 1,867,833
_RS08815
RS_RS08820: RS_RS08820 - intracellular septation protein A, at 1,867,833 to 1,868,393
_RS08820
RS_RS08825: RS_RS08825 - peptide-methionine (R)-S-oxide reductase, at 1,868,416 to 1,868,802
_RS08825
Position (kb)
1866
1867
1868 Strain fitness (log2 ratio)
-1
0
1 at 1866.153 kb on + strand at 1866.153 kb on + strand at 1866.153 kb on + strand at 1866.153 kb on + strand at 1866.153 kb on + strand at 1866.711 kb on + strand at 1866.711 kb on + strand at 1866.711 kb on + strand at 1866.952 kb on - strand, within RS_RS08810 at 1866.952 kb on - strand, within RS_RS08810 at 1867.114 kb on - strand, within RS_RS08810 at 1867.152 kb on + strand, within RS_RS08810 at 1867.197 kb on + strand, within RS_RS08810 at 1867.198 kb on - strand, within RS_RS08810 at 1867.198 kb on - strand, within RS_RS08810 at 1867.551 kb on - strand at 1867.628 kb on + strand, within RS_RS08815 at 1867.628 kb on + strand, within RS_RS08815 at 1867.628 kb on + strand, within RS_RS08815 at 1867.628 kb on + strand, within RS_RS08815 at 1867.629 kb on - strand, within RS_RS08815 at 1867.694 kb on + strand, within RS_RS08815 at 1867.695 kb on - strand, within RS_RS08815 at 1868.453 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 1,866,153 + -1.2 1,866,153 + -0.4 1,866,153 + +0.1 1,866,153 + -0.0 1,866,153 + +1.1 1,866,711 + -0.2 1,866,711 + +1.1 1,866,711 + +1.1 1,866,952 - RS_RS08810 0.30 -0.7 1,866,952 - RS_RS08810 0.30 -0.4 1,867,114 - RS_RS08810 0.51 -0.3 1,867,152 + RS_RS08810 0.55 +1.1 1,867,197 + RS_RS08810 0.61 -1.3 1,867,198 - RS_RS08810 0.61 +0.6 1,867,198 - RS_RS08810 0.61 -0.4 1,867,551 - -1.7 1,867,628 + RS_RS08815 0.28 +0.4 1,867,628 + RS_RS08815 0.28 +1.1 1,867,628 + RS_RS08815 0.28 -0.8 1,867,628 + RS_RS08815 0.28 -0.0 1,867,629 - RS_RS08815 0.28 -0.1 1,867,694 + RS_RS08815 0.51 -0.3 1,867,695 - RS_RS08815 0.51 +0.4 1,868,453 + -0.0
Or see this region's nucleotide sequence