Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS04600 and RS_RS04605 are separated by 66 nucleotides RS_RS04605 and RS_RS04610 are separated by 112 nucleotides RS_RS04610 and RS_RS04615 are separated by 247 nucleotides
RS_RS04600: RS_RS04600 - acyl-CoA dehydrogenase, at 976,328 to 978,112
_RS04600
RS_RS04605: RS_RS04605 - D-amino acid dehydrogenase small subunit, at 978,179 to 979,468
_RS04605
RS_RS04610: RS_RS04610 - AsnC family transcriptional regulator, at 979,581 to 980,078
_RS04610
RS_RS04615: RS_RS04615 - radical SAM protein, at 980,326 to 981,453
_RS04615
Position (kb) 978
979
980 Strain fitness (log2 ratio) -1
0
1 at 977.189 kb on + strand, within RS_RS04600 at 977.189 kb on + strand, within RS_RS04600 at 977.189 kb on + strand, within RS_RS04600 at 977.280 kb on - strand, within RS_RS04600 at 977.415 kb on - strand, within RS_RS04600 at 977.489 kb on + strand, within RS_RS04600 at 977.610 kb on - strand, within RS_RS04600 at 977.610 kb on - strand, within RS_RS04600 at 977.613 kb on - strand, within RS_RS04600 at 977.639 kb on + strand, within RS_RS04600 at 977.639 kb on + strand, within RS_RS04600 at 977.640 kb on - strand, within RS_RS04600 at 978.226 kb on - strand at 978.579 kb on + strand, within RS_RS04605 at 978.579 kb on + strand, within RS_RS04605 at 978.580 kb on - strand, within RS_RS04605 at 978.660 kb on + strand, within RS_RS04605 at 978.702 kb on + strand, within RS_RS04605 at 978.702 kb on + strand, within RS_RS04605 at 978.702 kb on + strand, within RS_RS04605 at 978.703 kb on - strand, within RS_RS04605 at 979.128 kb on + strand, within RS_RS04605 at 979.168 kb on - strand, within RS_RS04605 at 979.417 kb on - strand at 979.419 kb on + strand at 979.420 kb on - strand at 979.513 kb on + strand at 979.513 kb on + strand at 979.514 kb on - strand at 979.542 kb on + strand at 979.543 kb on - strand at 979.761 kb on + strand, within RS_RS04610 at 979.921 kb on - strand, within RS_RS04610 at 980.016 kb on + strand, within RS_RS04610 at 980.016 kb on + strand, within RS_RS04610 at 980.016 kb on + strand, within RS_RS04610 at 980.017 kb on - strand, within RS_RS04610 at 980.017 kb on - strand, within RS_RS04610 at 980.017 kb on - strand, within RS_RS04610
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 977,189 + RS_RS04600 0.48 -0.0 977,189 + RS_RS04600 0.48 +0.3 977,189 + RS_RS04600 0.48 -0.1 977,280 - RS_RS04600 0.53 -0.3 977,415 - RS_RS04600 0.61 -0.3 977,489 + RS_RS04600 0.65 -0.7 977,610 - RS_RS04600 0.72 -0.3 977,610 - RS_RS04600 0.72 +1.3 977,613 - RS_RS04600 0.72 -0.7 977,639 + RS_RS04600 0.73 +0.1 977,639 + RS_RS04600 0.73 -0.1 977,640 - RS_RS04600 0.74 -0.7 978,226 - +0.2 978,579 + RS_RS04605 0.31 +0.3 978,579 + RS_RS04605 0.31 -0.3 978,580 - RS_RS04605 0.31 +0.0 978,660 + RS_RS04605 0.37 +0.5 978,702 + RS_RS04605 0.41 +0.3 978,702 + RS_RS04605 0.41 +0.0 978,702 + RS_RS04605 0.41 -1.1 978,703 - RS_RS04605 0.41 +0.3 979,128 + RS_RS04605 0.74 +0.2 979,168 - RS_RS04605 0.77 +0.5 979,417 - +0.4 979,419 + +1.7 979,420 - +0.2 979,513 + +0.1 979,513 + +0.6 979,514 - -0.2 979,542 + -0.4 979,543 - -0.0 979,761 + RS_RS04610 0.36 +0.6 979,921 - RS_RS04610 0.68 +0.3 980,016 + RS_RS04610 0.87 +0.2 980,016 + RS_RS04610 0.87 +1.3 980,016 + RS_RS04610 0.87 +0.2 980,017 - RS_RS04610 0.88 +0.6 980,017 - RS_RS04610 0.88 +0.0 980,017 - RS_RS04610 0.88 +0.9
Or see this region's nucleotide sequence