Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS03350 and RS_RS03355 are separated by 223 nucleotides RS_RS03355 and RS_RS03360 are separated by 314 nucleotides RS_RS03360 and RS_RS03365 are separated by 31 nucleotides RS_RS03365 and RS_RS03370 are separated by 58 nucleotides
RS_RS03350: RS_RS03350 - glutamate-1-semialdehyde 2,1-aminomutase, at 711,010 to 712,311
_RS03350
RS_RS03355: RS_RS03355 - rubredoxin, at 712,535 to 712,705
_RS03355
RS_RS03360: RS_RS03360 - response regulator, at 713,020 to 713,463
_RS03360
RS_RS03365: RS_RS03365 - response regulator, at 713,495 to 713,863
_RS03365
RS_RS03370: RS_RS03370 - chemotaxis protein CheW, at 713,922 to 714,467
_RS03370
Position (kb) 713
714 Strain fitness (log2 ratio) -1
0
1 at 712.493 kb on + strand at 712.496 kb on + strand at 712.838 kb on + strand at 712.884 kb on + strand at 713.113 kb on - strand, within RS_RS03360 at 713.476 kb on - strand at 713.802 kb on - strand, within RS_RS03365 at 713.802 kb on - strand, within RS_RS03365 at 713.802 kb on - strand, within RS_RS03365 at 714.099 kb on + strand, within RS_RS03370 at 714.099 kb on + strand, within RS_RS03370 at 714.100 kb on - strand, within RS_RS03370 at 714.139 kb on - strand, within RS_RS03370
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 712,493 + -0.1 712,496 + +0.3 712,838 + +0.3 712,884 + -1.3 713,113 - RS_RS03360 0.21 +0.1 713,476 - -0.7 713,802 - RS_RS03365 0.83 -1.7 713,802 - RS_RS03365 0.83 -0.3 713,802 - RS_RS03365 0.83 -0.9 714,099 + RS_RS03370 0.32 +0.6 714,099 + RS_RS03370 0.32 -0.6 714,100 - RS_RS03370 0.33 -0.7 714,139 - RS_RS03370 0.40 -0.4
Or see this region's nucleotide sequence