Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS00860 and RS_RS00865 are separated by 160 nucleotides RS_RS00865 and RS_RS00870 are separated by 84 nucleotides
RS_RS00860: RS_RS00860 - 2-dehydropantoate 2-reductase, at 196,074 to 197,015
_RS00860
RS_RS00865: RS_RS00865 - tRNA 2-thiocytidine(32) synthetase TtcA, at 197,176 to 198,114
_RS00865
RS_RS00870: RS_RS00870 - hypothetical protein, at 198,199 to 199,098
_RS00870
Position (kb) 197
198
199 Strain fitness (log2 ratio) -1
0
1
2
3 at 196.347 kb on + strand, within RS_RS00860 at 196.348 kb on + strand, within RS_RS00860 at 196.348 kb on + strand, within RS_RS00860 at 196.349 kb on - strand, within RS_RS00860 at 196.349 kb on - strand, within RS_RS00860 at 196.349 kb on - strand, within RS_RS00860 at 196.777 kb on + strand, within RS_RS00860 at 196.777 kb on + strand, within RS_RS00860 at 197.002 kb on - strand at 197.052 kb on - strand at 197.052 kb on - strand at 197.308 kb on + strand, within RS_RS00865 at 197.308 kb on + strand, within RS_RS00865 at 197.308 kb on + strand, within RS_RS00865 at 197.308 kb on + strand, within RS_RS00865 at 197.309 kb on - strand, within RS_RS00865 at 197.309 kb on - strand, within RS_RS00865 at 197.426 kb on - strand, within RS_RS00865 at 197.449 kb on + strand, within RS_RS00865 at 197.449 kb on + strand, within RS_RS00865 at 197.449 kb on + strand, within RS_RS00865 at 197.450 kb on - strand, within RS_RS00865 at 197.474 kb on - strand, within RS_RS00865 at 197.551 kb on + strand, within RS_RS00865 at 197.551 kb on + strand, within RS_RS00865 at 197.720 kb on - strand, within RS_RS00865 at 197.998 kb on + strand, within RS_RS00865 at 198.393 kb on - strand, within RS_RS00870 at 198.710 kb on + strand, within RS_RS00870 at 198.710 kb on + strand, within RS_RS00870 at 198.711 kb on - strand, within RS_RS00870 at 198.833 kb on + strand, within RS_RS00870 at 199.018 kb on + strand at 199.019 kb on - strand at 199.019 kb on - strand at 199.022 kb on + strand at 199.022 kb on + strand at 199.022 kb on + strand at 199.022 kb on + strand at 199.023 kb on - strand at 199.023 kb on - strand at 199.023 kb on - strand at 199.047 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 196,347 + RS_RS00860 0.29 +0.5 196,348 + RS_RS00860 0.29 +0.2 196,348 + RS_RS00860 0.29 -0.5 196,349 - RS_RS00860 0.29 -0.1 196,349 - RS_RS00860 0.29 +0.1 196,349 - RS_RS00860 0.29 -0.5 196,777 + RS_RS00860 0.75 -0.5 196,777 + RS_RS00860 0.75 -0.1 197,002 - -0.3 197,052 - -1.0 197,052 - +0.5 197,308 + RS_RS00865 0.14 -0.3 197,308 + RS_RS00865 0.14 -0.5 197,308 + RS_RS00865 0.14 -0.4 197,308 + RS_RS00865 0.14 +0.4 197,309 - RS_RS00865 0.14 -0.7 197,309 - RS_RS00865 0.14 -0.2 197,426 - RS_RS00865 0.27 -0.1 197,449 + RS_RS00865 0.29 +0.5 197,449 + RS_RS00865 0.29 +3.1 197,449 + RS_RS00865 0.29 +0.0 197,450 - RS_RS00865 0.29 +0.3 197,474 - RS_RS00865 0.32 +1.5 197,551 + RS_RS00865 0.40 +1.5 197,551 + RS_RS00865 0.40 -1.7 197,720 - RS_RS00865 0.58 -0.1 197,998 + RS_RS00865 0.88 +1.5 198,393 - RS_RS00870 0.22 -0.2 198,710 + RS_RS00870 0.57 +0.3 198,710 + RS_RS00870 0.57 -0.2 198,711 - RS_RS00870 0.57 +0.3 198,833 + RS_RS00870 0.70 +0.0 199,018 + +0.1 199,019 - +0.1 199,019 - +0.5 199,022 + -0.4 199,022 + -0.3 199,022 + +0.5 199,022 + -1.1 199,023 - -0.0 199,023 - +0.6 199,023 - +1.5 199,047 - -0.1
Or see this region's nucleotide sequence