Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS15580

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS15575 and RS_RS25665 overlap by 65 nucleotidesRS_RS25665 and RS_RS15580 are separated by 188 nucleotidesRS_RS15580 and RS_RS15585 are separated by 160 nucleotides RS_RS15575: RS_RS15575 - serine protease, at 3,338,217 to 3,340,211 _RS15575 RS_RS25665: RS_RS25665 - hypothetical protein, at 3,340,147 to 3,340,355 _RS25665 RS_RS15580: RS_RS15580 - Lrp/AsnC family transcriptional regulator, at 3,340,544 to 3,341,032 _RS15580 RS_RS15585: RS_RS15585 - 4-hydroxyphenylpyruvate dioxygenase, at 3,341,193 to 3,342,296 _RS15585 Position (kb) 3340 3341 3342Strain fitness (log2 ratio) -1 0 1at 3339.643 kb on + strand, within RS_RS15575at 3339.643 kb on + strand, within RS_RS15575at 3339.643 kb on + strand, within RS_RS15575at 3339.643 kb on + strand, within RS_RS15575at 3339.644 kb on - strand, within RS_RS15575at 3339.644 kb on - strand, within RS_RS15575at 3339.644 kb on - strand, within RS_RS15575at 3339.644 kb on - strand, within RS_RS15575at 3339.776 kb on - strand, within RS_RS15575at 3339.904 kb on + strand, within RS_RS15575at 3339.905 kb on - strand, within RS_RS15575at 3340.065 kb on - strandat 3340.261 kb on - strand, within RS_RS25665at 3340.310 kb on + strand, within RS_RS25665at 3340.312 kb on + strand, within RS_RS25665at 3340.313 kb on - strand, within RS_RS25665at 3340.313 kb on - strand, within RS_RS25665at 3340.335 kb on - strandat 3340.570 kb on + strandat 3340.571 kb on - strandat 3340.571 kb on - strandat 3340.692 kb on + strand, within RS_RS15580at 3340.693 kb on - strand, within RS_RS15580at 3340.693 kb on - strand, within RS_RS15580at 3340.716 kb on + strand, within RS_RS15580at 3340.821 kb on + strand, within RS_RS15580at 3340.822 kb on - strand, within RS_RS15580at 3341.126 kb on + strandat 3341.126 kb on + strandat 3341.126 kb on + strandat 3341.126 kb on + strandat 3341.250 kb on + strandat 3341.343 kb on + strand, within RS_RS15585at 3341.343 kb on + strand, within RS_RS15585at 3341.344 kb on - strand, within RS_RS15585at 3341.469 kb on + strand, within RS_RS15585at 3341.469 kb on + strand, within RS_RS15585at 3341.572 kb on - strand, within RS_RS15585at 3341.572 kb on - strand, within RS_RS15585at 3341.634 kb on - strand, within RS_RS15585at 3341.637 kb on + strand, within RS_RS15585at 3341.637 kb on + strand, within RS_RS15585at 3341.637 kb on + strand, within RS_RS15585at 3341.638 kb on - strand, within RS_RS15585at 3341.701 kb on - strand, within RS_RS15585at 3341.727 kb on + strand, within RS_RS15585at 3341.727 kb on + strand, within RS_RS15585at 3341.760 kb on + strand, within RS_RS15585at 3341.761 kb on - strand, within RS_RS15585at 3341.925 kb on + strand, within RS_RS15585at 3341.925 kb on + strand, within RS_RS15585at 3341.925 kb on + strand, within RS_RS15585at 3341.926 kb on - strand, within RS_RS15585at 3341.926 kb on - strand, within RS_RS15585

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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3,339,643 + RS_RS15575 0.71 +0.2
3,339,643 + RS_RS15575 0.71 -0.3
3,339,643 + RS_RS15575 0.71 +0.7
3,339,643 + RS_RS15575 0.71 +0.2
3,339,644 - RS_RS15575 0.72 -0.1
3,339,644 - RS_RS15575 0.72 +0.3
3,339,644 - RS_RS15575 0.72 -0.2
3,339,644 - RS_RS15575 0.72 +0.1
3,339,776 - RS_RS15575 0.78 -0.3
3,339,904 + RS_RS15575 0.85 +1.4
3,339,905 - RS_RS15575 0.85 -0.9
3,340,065 - -0.6
3,340,261 - RS_RS25665 0.55 -0.3
3,340,310 + RS_RS25665 0.78 +1.7
3,340,312 + RS_RS25665 0.79 -0.6
3,340,313 - RS_RS25665 0.79 +1.7
3,340,313 - RS_RS25665 0.79 -0.1
3,340,335 - +0.2
3,340,570 + -0.1
3,340,571 - -0.4
3,340,571 - +1.7
3,340,692 + RS_RS15580 0.30 +0.1
3,340,693 - RS_RS15580 0.30 +0.7
3,340,693 - RS_RS15580 0.30 +0.0
3,340,716 + RS_RS15580 0.35 +0.2
3,340,821 + RS_RS15580 0.57 +0.3
3,340,822 - RS_RS15580 0.57 -1.0
3,341,126 + -0.3
3,341,126 + +0.0
3,341,126 + -0.1
3,341,126 + +1.4
3,341,250 + -0.6
3,341,343 + RS_RS15585 0.14 +0.2
3,341,343 + RS_RS15585 0.14 +0.0
3,341,344 - RS_RS15585 0.14 +0.1
3,341,469 + RS_RS15585 0.25 +1.7
3,341,469 + RS_RS15585 0.25 +0.7
3,341,572 - RS_RS15585 0.34 -0.1
3,341,572 - RS_RS15585 0.34 +0.9
3,341,634 - RS_RS15585 0.40 +0.3
3,341,637 + RS_RS15585 0.40 -0.5
3,341,637 + RS_RS15585 0.40 +0.1
3,341,637 + RS_RS15585 0.40 -0.1
3,341,638 - RS_RS15585 0.40 +1.7
3,341,701 - RS_RS15585 0.46 -0.6
3,341,727 + RS_RS15585 0.48 -0.2
3,341,727 + RS_RS15585 0.48 +0.7
3,341,760 + RS_RS15585 0.51 +0.1
3,341,761 - RS_RS15585 0.51 -0.8
3,341,925 + RS_RS15585 0.66 +0.2
3,341,925 + RS_RS15585 0.66 +0.6
3,341,925 + RS_RS15585 0.66 +0.5
3,341,926 - RS_RS15585 0.66 -0.6
3,341,926 - RS_RS15585 0.66 +1.7

Or see this region's nucleotide sequence