Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS15575 and RS_RS25665 overlap by 65 nucleotides RS_RS25665 and RS_RS15580 are separated by 188 nucleotides RS_RS15580 and RS_RS15585 are separated by 160 nucleotides
RS_RS15575: RS_RS15575 - serine protease, at 3,338,217 to 3,340,211
_RS15575
RS_RS25665: RS_RS25665 - hypothetical protein, at 3,340,147 to 3,340,355
_RS25665
RS_RS15580: RS_RS15580 - Lrp/AsnC family transcriptional regulator, at 3,340,544 to 3,341,032
_RS15580
RS_RS15585: RS_RS15585 - 4-hydroxyphenylpyruvate dioxygenase, at 3,341,193 to 3,342,296
_RS15585
Position (kb)
3340
3341
3342 Strain fitness (log2 ratio)
-1
0
1 at 3339.643 kb on + strand, within RS_RS15575 at 3339.643 kb on + strand, within RS_RS15575 at 3339.643 kb on + strand, within RS_RS15575 at 3339.643 kb on + strand, within RS_RS15575 at 3339.644 kb on - strand, within RS_RS15575 at 3339.644 kb on - strand, within RS_RS15575 at 3339.644 kb on - strand, within RS_RS15575 at 3339.644 kb on - strand, within RS_RS15575 at 3339.776 kb on - strand, within RS_RS15575 at 3339.904 kb on + strand, within RS_RS15575 at 3339.905 kb on - strand, within RS_RS15575 at 3340.065 kb on - strand at 3340.261 kb on - strand, within RS_RS25665 at 3340.310 kb on + strand, within RS_RS25665 at 3340.312 kb on + strand, within RS_RS25665 at 3340.313 kb on - strand, within RS_RS25665 at 3340.313 kb on - strand, within RS_RS25665 at 3340.335 kb on - strand at 3340.570 kb on + strand at 3340.571 kb on - strand at 3340.571 kb on - strand at 3340.692 kb on + strand, within RS_RS15580 at 3340.693 kb on - strand, within RS_RS15580 at 3340.693 kb on - strand, within RS_RS15580 at 3340.716 kb on + strand, within RS_RS15580 at 3340.821 kb on + strand, within RS_RS15580 at 3340.822 kb on - strand, within RS_RS15580 at 3341.126 kb on + strand at 3341.126 kb on + strand at 3341.126 kb on + strand at 3341.126 kb on + strand at 3341.250 kb on + strand at 3341.343 kb on + strand, within RS_RS15585 at 3341.343 kb on + strand, within RS_RS15585 at 3341.344 kb on - strand, within RS_RS15585 at 3341.469 kb on + strand, within RS_RS15585 at 3341.469 kb on + strand, within RS_RS15585 at 3341.572 kb on - strand, within RS_RS15585 at 3341.572 kb on - strand, within RS_RS15585 at 3341.634 kb on - strand, within RS_RS15585 at 3341.637 kb on + strand, within RS_RS15585 at 3341.637 kb on + strand, within RS_RS15585 at 3341.637 kb on + strand, within RS_RS15585 at 3341.638 kb on - strand, within RS_RS15585 at 3341.701 kb on - strand, within RS_RS15585 at 3341.727 kb on + strand, within RS_RS15585 at 3341.727 kb on + strand, within RS_RS15585 at 3341.760 kb on + strand, within RS_RS15585 at 3341.761 kb on - strand, within RS_RS15585 at 3341.925 kb on + strand, within RS_RS15585 at 3341.925 kb on + strand, within RS_RS15585 at 3341.925 kb on + strand, within RS_RS15585 at 3341.926 kb on - strand, within RS_RS15585 at 3341.926 kb on - strand, within RS_RS15585
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 3,339,643 + RS_RS15575 0.71 +0.2 3,339,643 + RS_RS15575 0.71 -0.3 3,339,643 + RS_RS15575 0.71 +0.7 3,339,643 + RS_RS15575 0.71 +0.2 3,339,644 - RS_RS15575 0.72 -0.1 3,339,644 - RS_RS15575 0.72 +0.3 3,339,644 - RS_RS15575 0.72 -0.2 3,339,644 - RS_RS15575 0.72 +0.1 3,339,776 - RS_RS15575 0.78 -0.3 3,339,904 + RS_RS15575 0.85 +1.4 3,339,905 - RS_RS15575 0.85 -0.9 3,340,065 - -0.6 3,340,261 - RS_RS25665 0.55 -0.3 3,340,310 + RS_RS25665 0.78 +1.7 3,340,312 + RS_RS25665 0.79 -0.6 3,340,313 - RS_RS25665 0.79 +1.7 3,340,313 - RS_RS25665 0.79 -0.1 3,340,335 - +0.2 3,340,570 + -0.1 3,340,571 - -0.4 3,340,571 - +1.7 3,340,692 + RS_RS15580 0.30 +0.1 3,340,693 - RS_RS15580 0.30 +0.7 3,340,693 - RS_RS15580 0.30 +0.0 3,340,716 + RS_RS15580 0.35 +0.2 3,340,821 + RS_RS15580 0.57 +0.3 3,340,822 - RS_RS15580 0.57 -1.0 3,341,126 + -0.3 3,341,126 + +0.0 3,341,126 + -0.1 3,341,126 + +1.4 3,341,250 + -0.6 3,341,343 + RS_RS15585 0.14 +0.2 3,341,343 + RS_RS15585 0.14 +0.0 3,341,344 - RS_RS15585 0.14 +0.1 3,341,469 + RS_RS15585 0.25 +1.7 3,341,469 + RS_RS15585 0.25 +0.7 3,341,572 - RS_RS15585 0.34 -0.1 3,341,572 - RS_RS15585 0.34 +0.9 3,341,634 - RS_RS15585 0.40 +0.3 3,341,637 + RS_RS15585 0.40 -0.5 3,341,637 + RS_RS15585 0.40 +0.1 3,341,637 + RS_RS15585 0.40 -0.1 3,341,638 - RS_RS15585 0.40 +1.7 3,341,701 - RS_RS15585 0.46 -0.6 3,341,727 + RS_RS15585 0.48 -0.2 3,341,727 + RS_RS15585 0.48 +0.7 3,341,760 + RS_RS15585 0.51 +0.1 3,341,761 - RS_RS15585 0.51 -0.8 3,341,925 + RS_RS15585 0.66 +0.2 3,341,925 + RS_RS15585 0.66 +0.6 3,341,925 + RS_RS15585 0.66 +0.5 3,341,926 - RS_RS15585 0.66 -0.6 3,341,926 - RS_RS15585 0.66 +1.7
Or see this region's nucleotide sequence