Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS13545 and RS_RS13550 are separated by 141 nucleotides RS_RS13550 and RS_RS25625 are separated by 120 nucleotides RS_RS25625 and RS_RS13555 are separated by 223 nucleotides RS_RS13555 and RS_RS13560 are separated by 11 nucleotides
RS_RS13545: RS_RS13545 - LysR family transcriptional regulator, at 2,912,188 to 2,913,045
_RS13545
RS_RS13550: RS_RS13550 - DSD1 family PLP-dependent enzyme, at 2,913,187 to 2,914,317
_RS13550
RS_RS25625: RS_RS25625 - hypothetical protein, at 2,914,438 to 2,914,635
_RS25625
RS_RS13555: RS_RS13555 - hypothetical protein, at 2,914,859 to 2,915,299
_RS13555
RS_RS13560: RS_RS13560 - hypothetical protein, at 2,915,311 to 2,915,514
_RS13560
Position (kb)
2913
2914
2915 Strain fitness (log2 ratio)
-1
0
1 at 2912.729 kb on + strand, within RS_RS13545 at 2912.729 kb on + strand, within RS_RS13545 at 2913.056 kb on - strand at 2913.056 kb on - strand at 2913.113 kb on + strand at 2913.113 kb on + strand at 2913.114 kb on - strand at 2913.706 kb on + strand, within RS_RS13550 at 2913.706 kb on + strand, within RS_RS13550 at 2913.889 kb on + strand, within RS_RS13550 at 2913.889 kb on + strand, within RS_RS13550 at 2913.889 kb on + strand, within RS_RS13550 at 2913.890 kb on - strand, within RS_RS13550 at 2913.890 kb on - strand, within RS_RS13550 at 2914.258 kb on + strand at 2914.307 kb on - strand at 2914.600 kb on + strand, within RS_RS25625 at 2914.673 kb on + strand at 2914.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 2,912,729 + RS_RS13545 0.63 -0.9 2,912,729 + RS_RS13545 0.63 -0.1 2,913,056 - -0.0 2,913,056 - +0.3 2,913,113 + -0.2 2,913,113 + +0.0 2,913,114 - -0.1 2,913,706 + RS_RS13550 0.46 -1.5 2,913,706 + RS_RS13550 0.46 +1.0 2,913,889 + RS_RS13550 0.62 -0.5 2,913,889 + RS_RS13550 0.62 +0.4 2,913,889 + RS_RS13550 0.62 -0.7 2,913,890 - RS_RS13550 0.62 +0.2 2,913,890 - RS_RS13550 0.62 -0.5 2,914,258 + -0.7 2,914,307 - -1.6 2,914,600 + RS_RS25625 0.82 -1.3 2,914,673 + -0.5 2,914,673 + -0.7
Or see this region's nucleotide sequence