Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS11000

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS10995 and RS_RS11000 are separated by 148 nucleotidesRS_RS11000 and RS_RS11005 overlap by 4 nucleotidesRS_RS11005 and RS_RS11010 are separated by 50 nucleotides RS_RS10995: RS_RS10995 - phosphoribosylamine--glycine ligase, at 2,373,410 to 2,374,678 _RS10995 RS_RS11000: RS_RS11000 - coproporphyrinogen III oxidase, at 2,374,827 to 2,375,735 _RS11000 RS_RS11005: RS_RS11005 - nicotinate-nucleotide adenylyltransferase, at 2,375,732 to 2,376,427 _RS11005 RS_RS11010: RS_RS11010 - ribosome silencing factor, at 2,376,478 to 2,377,089 _RS11010 Position (kb) 2374 2375 2376Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2376.014 kb on + strand, within RS_RS11005at 2376.140 kb on + strand, within RS_RS11005at 2376.243 kb on + strand, within RS_RS11005at 2376.243 kb on + strand, within RS_RS11005at 2376.244 kb on - strand, within RS_RS11005at 2376.244 kb on - strand, within RS_RS11005at 2376.396 kb on - strandat 2376.470 kb on + strandat 2376.495 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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2,376,014 + RS_RS11005 0.41 -0.6
2,376,140 + RS_RS11005 0.59 -2.2
2,376,243 + RS_RS11005 0.73 +1.4
2,376,243 + RS_RS11005 0.73 -3.8
2,376,244 - RS_RS11005 0.74 +0.4
2,376,244 - RS_RS11005 0.74 -2.8
2,376,396 - -0.7
2,376,470 + +0.1
2,376,495 - -1.5

Or see this region's nucleotide sequence