Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS04715

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS04710 and RS_RS04715 are separated by 207 nucleotidesRS_RS04715 and RS_RS04720 overlap by 4 nucleotides RS_RS04705: RS_RS04705 - dihydrofolate reductase, at 994,374 to 994,877 _RS04705 RS_RS04710: RS_RS04710 - thymidylate synthase, at 994,874 to 995,668 _RS04710 RS_RS04715: RS_RS04715 - serine/threonine dehydratase, at 995,876 to 996,880 _RS04715 RS_RS04720: RS_RS04720 - membrane protein, at 996,877 to 997,569 _RS04720 Position (kb) 995 996 997Strain fitness (log2 ratio) -1 0 1 2 3at 995.706 kb on + strandat 995.739 kb on + strandat 996.043 kb on + strand, within RS_RS04715at 996.044 kb on - strand, within RS_RS04715at 996.083 kb on + strand, within RS_RS04715at 996.083 kb on + strand, within RS_RS04715at 996.383 kb on + strand, within RS_RS04715at 996.384 kb on - strand, within RS_RS04715at 996.536 kb on + strand, within RS_RS04715at 996.536 kb on + strand, within RS_RS04715at 996.536 kb on + strand, within RS_RS04715at 996.537 kb on - strand, within RS_RS04715at 996.537 kb on - strand, within RS_RS04715at 996.542 kb on + strand, within RS_RS04715at 996.542 kb on + strand, within RS_RS04715at 996.543 kb on - strand, within RS_RS04715at 996.707 kb on + strand, within RS_RS04715at 997.764 kb on + strandat 997.764 kb on + strandat 997.764 kb on + strandat 997.764 kb on + strandat 997.790 kb on - strandat 997.792 kb on - strandat 997.792 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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995,706 + +0.6
995,739 + +0.1
996,043 + RS_RS04715 0.17 -0.0
996,044 - RS_RS04715 0.17 +0.4
996,083 + RS_RS04715 0.21 -0.1
996,083 + RS_RS04715 0.21 +0.7
996,383 + RS_RS04715 0.50 +0.7
996,384 - RS_RS04715 0.51 -0.2
996,536 + RS_RS04715 0.66 -0.9
996,536 + RS_RS04715 0.66 -0.3
996,536 + RS_RS04715 0.66 -0.2
996,537 - RS_RS04715 0.66 +0.1
996,537 - RS_RS04715 0.66 +0.0
996,542 + RS_RS04715 0.66 -0.8
996,542 + RS_RS04715 0.66 -0.1
996,543 - RS_RS04715 0.66 +3.0
996,707 + RS_RS04715 0.83 -0.1
997,764 + -0.9
997,764 + -0.7
997,764 + +1.4
997,764 + +1.4
997,790 - -0.2
997,792 - +2.4
997,792 - +0.1

Or see this region's nucleotide sequence