Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS04700 and RS_RS04705 are separated by 79 nucleotides RS_RS04705 and RS_RS04710 overlap by 4 nucleotides RS_RS04710 and RS_RS04715 are separated by 207 nucleotides
RS_RS04700: RS_RS04700 - metalloprotease PmbA, at 992,921 to 994,294
_RS04700
RS_RS04705: RS_RS04705 - dihydrofolate reductase, at 994,374 to 994,877
_RS04705
RS_RS04710: RS_RS04710 - thymidylate synthase, at 994,874 to 995,668
_RS04710
RS_RS04715: RS_RS04715 - serine/threonine dehydratase, at 995,876 to 996,880
_RS04715
Position (kb) 994
995
996 Strain fitness (log2 ratio) -1
0
1
2
3 at 993.923 kb on + strand, within RS_RS04700 at 993.924 kb on - strand, within RS_RS04700 at 994.088 kb on + strand, within RS_RS04700 at 994.089 kb on - strand, within RS_RS04700 at 994.089 kb on - strand, within RS_RS04700 at 994.089 kb on - strand, within RS_RS04700 at 994.292 kb on + strand at 995.706 kb on + strand at 995.739 kb on + strand at 996.043 kb on + strand, within RS_RS04715 at 996.044 kb on - strand, within RS_RS04715 at 996.083 kb on + strand, within RS_RS04715 at 996.083 kb on + strand, within RS_RS04715 at 996.383 kb on + strand, within RS_RS04715 at 996.384 kb on - strand, within RS_RS04715 at 996.536 kb on + strand, within RS_RS04715 at 996.536 kb on + strand, within RS_RS04715 at 996.536 kb on + strand, within RS_RS04715 at 996.537 kb on - strand, within RS_RS04715 at 996.537 kb on - strand, within RS_RS04715 at 996.542 kb on + strand, within RS_RS04715 at 996.542 kb on + strand, within RS_RS04715 at 996.543 kb on - strand, within RS_RS04715
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 993,923 + RS_RS04700 0.73 +0.8 993,924 - RS_RS04700 0.73 +0.4 994,088 + RS_RS04700 0.85 +1.4 994,089 - RS_RS04700 0.85 +0.4 994,089 - RS_RS04700 0.85 +0.3 994,089 - RS_RS04700 0.85 +0.1 994,292 + +1.0 995,706 + +0.6 995,739 + +0.1 996,043 + RS_RS04715 0.17 -0.0 996,044 - RS_RS04715 0.17 +0.4 996,083 + RS_RS04715 0.21 -0.1 996,083 + RS_RS04715 0.21 +0.7 996,383 + RS_RS04715 0.50 +0.7 996,384 - RS_RS04715 0.51 -0.2 996,536 + RS_RS04715 0.66 -0.9 996,536 + RS_RS04715 0.66 -0.3 996,536 + RS_RS04715 0.66 -0.2 996,537 - RS_RS04715 0.66 +0.1 996,537 - RS_RS04715 0.66 +0.0 996,542 + RS_RS04715 0.66 -0.8 996,542 + RS_RS04715 0.66 -0.1 996,543 - RS_RS04715 0.66 +3.0
Or see this region's nucleotide sequence