Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS04615 and RS_RS04620 are separated by 1 nucleotides RS_RS04620 and RS_RS04625 are separated by 204 nucleotides RS_RS04625 and RS_RS04630 are separated by 119 nucleotides RS_RS04630 and RS_RS04635 are separated by 182 nucleotides
RS_RS04615: RS_RS04615 - radical SAM protein, at 980,326 to 981,453
_RS04615
RS_RS04620: RS_RS04620 - NINE protein, at 981,455 to 981,910
_RS04620
RS_RS04625: RS_RS04625 - hypothetical protein, at 982,115 to 982,297
_RS04625
RS_RS04630: RS_RS04630 - hypothetical protein, at 982,417 to 982,821
_RS04630
RS_RS04635: RS_RS04635 - sorbosone dehydrogenase, at 983,004 to 984,077
_RS04635
Position (kb)
982
983 Strain fitness (log2 ratio)
-1
0
1 at 981.573 kb on - strand, within RS_RS04620 at 981.651 kb on + strand, within RS_RS04620 at 981.652 kb on - strand, within RS_RS04620 at 981.822 kb on + strand, within RS_RS04620 at 981.904 kb on + strand at 981.904 kb on + strand at 981.904 kb on + strand at 981.904 kb on + strand at 981.904 kb on + strand at 981.904 kb on + strand at 981.905 kb on - strand at 981.957 kb on + strand at 982.051 kb on - strand at 982.117 kb on - strand at 982.348 kb on + strand at 982.349 kb on - strand at 982.349 kb on - strand at 982.920 kb on + strand at 982.921 kb on - strand at 982.921 kb on - strand at 982.921 kb on - strand at 983.176 kb on - strand, within RS_RS04635 at 983.281 kb on + strand, within RS_RS04635 at 983.338 kb on + strand, within RS_RS04635 at 983.338 kb on + strand, within RS_RS04635 at 983.341 kb on - strand, within RS_RS04635 at 983.602 kb on + strand, within RS_RS04635 at 983.653 kb on + strand, within RS_RS04635 at 983.653 kb on + strand, within RS_RS04635 at 983.653 kb on + strand, within RS_RS04635 at 983.653 kb on + strand, within RS_RS04635 at 983.653 kb on + strand, within RS_RS04635 at 983.653 kb on + strand, within RS_RS04635 at 983.654 kb on - strand, within RS_RS04635 at 983.654 kb on - strand, within RS_RS04635 at 983.654 kb on - strand, within RS_RS04635 at 983.654 kb on - strand, within RS_RS04635 at 983.654 kb on - strand, within RS_RS04635 at 983.654 kb on - strand, within RS_RS04635 at 983.654 kb on - strand, within RS_RS04635 at 983.654 kb on - strand, within RS_RS04635 at 983.803 kb on + strand, within RS_RS04635 at 983.803 kb on + strand, within RS_RS04635 at 983.803 kb on + strand, within RS_RS04635 at 983.803 kb on + strand, within RS_RS04635 at 983.803 kb on + strand, within RS_RS04635 at 983.803 kb on + strand, within RS_RS04635 at 983.804 kb on - strand, within RS_RS04635
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 981,573 - RS_RS04620 0.26 -0.4 981,651 + RS_RS04620 0.43 -0.2 981,652 - RS_RS04620 0.43 +1.4 981,822 + RS_RS04620 0.80 +1.0 981,904 + -0.3 981,904 + -1.1 981,904 + -0.6 981,904 + -0.4 981,904 + -0.1 981,904 + +1.4 981,905 - +0.2 981,957 + -0.1 982,051 - +0.1 982,117 - -0.6 982,348 + +0.3 982,349 - -0.0 982,349 - -1.0 982,920 + -0.1 982,921 - +0.0 982,921 - +0.4 982,921 - +0.4 983,176 - RS_RS04635 0.16 -0.0 983,281 + RS_RS04635 0.26 -0.6 983,338 + RS_RS04635 0.31 -0.3 983,338 + RS_RS04635 0.31 +1.4 983,341 - RS_RS04635 0.31 +0.2 983,602 + RS_RS04635 0.56 -0.2 983,653 + RS_RS04635 0.60 +0.4 983,653 + RS_RS04635 0.60 -0.2 983,653 + RS_RS04635 0.60 -0.1 983,653 + RS_RS04635 0.60 +0.0 983,653 + RS_RS04635 0.60 -0.2 983,653 + RS_RS04635 0.60 -1.6 983,654 - RS_RS04635 0.61 -1.4 983,654 - RS_RS04635 0.61 -0.4 983,654 - RS_RS04635 0.61 -0.7 983,654 - RS_RS04635 0.61 -1.1 983,654 - RS_RS04635 0.61 -1.7 983,654 - RS_RS04635 0.61 -0.6 983,654 - RS_RS04635 0.61 -0.7 983,654 - RS_RS04635 0.61 +1.4 983,803 + RS_RS04635 0.74 +0.0 983,803 + RS_RS04635 0.74 -0.5 983,803 + RS_RS04635 0.74 +1.4 983,803 + RS_RS04635 0.74 -0.5 983,803 + RS_RS04635 0.74 -1.3 983,803 + RS_RS04635 0.74 +1.4 983,804 - RS_RS04635 0.74 +1.4
Or see this region's nucleotide sequence