Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS02130

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS02125 and RS_RS02130 are separated by 3 nucleotidesRS_RS02130 and RS_RS02135 are separated by 3 nucleotidesRS_RS02135 and RS_RS02140 overlap by 4 nucleotides RS_RS02125: RS_RS02125 - membrane protein, at 456,068 to 458,551 _RS02125 RS_RS02130: RS_RS02130 - outer membrane lipoprotein carrier protein LolA, at 458,555 to 459,190 _RS02130 RS_RS02135: RS_RS02135 - acyl-CoA synthetase, at 459,194 to 460,165 _RS02135 RS_RS02140: RS_RS02140 - beta-hydroxyacyl-ACP dehydratase, at 460,162 to 461,853 _RS02140 Position (kb) 458 459 460Strain fitness (log2 ratio) -1 0 1 2at 457.555 kb on - strand, within RS_RS02125at 457.563 kb on + strand, within RS_RS02125at 457.564 kb on - strand, within RS_RS02125at 458.743 kb on - strand, within RS_RS02130at 458.838 kb on + strand, within RS_RS02130at 458.839 kb on - strand, within RS_RS02130at 458.988 kb on + strand, within RS_RS02130at 458.989 kb on - strand, within RS_RS02130at 459.285 kb on + strandat 459.285 kb on + strandat 459.285 kb on + strandat 459.445 kb on - strand, within RS_RS02135at 459.726 kb on + strand, within RS_RS02135at 459.727 kb on - strand, within RS_RS02135at 459.904 kb on - strand, within RS_RS02135at 459.948 kb on + strand, within RS_RS02135at 460.039 kb on - strand, within RS_RS02135

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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457,555 - RS_RS02125 0.60 -0.6
457,563 + RS_RS02125 0.60 -0.1
457,564 - RS_RS02125 0.60 -0.7
458,743 - RS_RS02130 0.30 -0.6
458,838 + RS_RS02130 0.44 +2.6
458,839 - RS_RS02130 0.45 +0.6
458,988 + RS_RS02130 0.68 -0.2
458,989 - RS_RS02130 0.68 +1.6
459,285 + -0.4
459,285 + -0.3
459,285 + +0.1
459,445 - RS_RS02135 0.26 -0.5
459,726 + RS_RS02135 0.55 -0.7
459,727 - RS_RS02135 0.55 -0.3
459,904 - RS_RS02135 0.73 -0.0
459,948 + RS_RS02135 0.78 +0.6
460,039 - RS_RS02135 0.87 -0.3

Or see this region's nucleotide sequence