Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS02115

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS02105 and RS_RS02110 overlap by 4 nucleotidesRS_RS02110 and RS_RS02115 overlap by 4 nucleotidesRS_RS02115 and RS_RS02120 are separated by 83 nucleotidesRS_RS02120 and RS_RS02125 overlap by 4 nucleotides RS_RS02105: RS_RS02105 - hypothetical protein, at 452,583 to 453,092 _RS02105 RS_RS02110: RS_RS02110 - 3-oxoacyl-ACP synthase, at 453,089 to 453,910 _RS02110 RS_RS02115: RS_RS02115 - beta-ketoacyl-[acyl-carrier-protein] synthase II, at 453,907 to 455,124 _RS02115 RS_RS02120: RS_RS02120 - polysaccharide deacetylase family protein, at 455,208 to 456,071 _RS02120 RS_RS02125: RS_RS02125 - membrane protein, at 456,068 to 458,551 _RS02125 Position (kb) 453 454 455 456Strain fitness (log2 ratio) -1 0 1at 454.259 kb on + strand, within RS_RS02115at 454.259 kb on + strand, within RS_RS02115at 454.259 kb on + strand, within RS_RS02115at 454.260 kb on - strand, within RS_RS02115at 455.307 kb on + strand, within RS_RS02120

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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454,259 + RS_RS02115 0.29 -0.4
454,259 + RS_RS02115 0.29 -0.4
454,259 + RS_RS02115 0.29 -0.4
454,260 - RS_RS02115 0.29 -0.0
455,307 + RS_RS02120 0.11 -1.1

Or see this region's nucleotide sequence