Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS01625 and RS_RS01630 are separated by 103 nucleotides RS_RS01630 and RS_RS01635 are separated by 25 nucleotides
RS_RS01625: RS_RS01625 - C4-dicarboxylate transporter 1, at 361,146 to 362,432
_RS01625
RS_RS01630: RS_RS01630 - sensor histidine kinase, at 362,536 to 364,533
_RS01630
RS_RS01635: RS_RS01635 - sigma-54-dependent Fis family transcriptional regulator, at 364,559 to 365,875
_RS01635
Position (kb) 362
363
364
365 Strain fitness (log2 ratio) -2
-1
0
1 at 361.776 kb on + strand, within RS_RS01625 at 361.825 kb on - strand, within RS_RS01625 at 361.909 kb on - strand, within RS_RS01625 at 361.909 kb on - strand, within RS_RS01625 at 362.067 kb on + strand, within RS_RS01625 at 362.067 kb on + strand, within RS_RS01625 at 362.067 kb on + strand, within RS_RS01625 at 362.067 kb on + strand, within RS_RS01625 at 362.068 kb on - strand, within RS_RS01625 at 362.068 kb on - strand, within RS_RS01625 at 362.068 kb on - strand, within RS_RS01625 at 362.482 kb on + strand at 362.483 kb on - strand at 362.483 kb on - strand at 362.483 kb on - strand at 362.791 kb on + strand, within RS_RS01630 at 362.830 kb on + strand, within RS_RS01630 at 362.917 kb on + strand, within RS_RS01630 at 362.959 kb on + strand, within RS_RS01630 at 362.960 kb on - strand, within RS_RS01630 at 362.960 kb on - strand, within RS_RS01630 at 363.002 kb on - strand, within RS_RS01630 at 363.035 kb on - strand, within RS_RS01630 at 363.089 kb on - strand, within RS_RS01630 at 363.122 kb on - strand, within RS_RS01630 at 363.124 kb on + strand, within RS_RS01630 at 363.125 kb on - strand, within RS_RS01630 at 363.125 kb on - strand, within RS_RS01630 at 363.125 kb on - strand, within RS_RS01630 at 363.286 kb on + strand, within RS_RS01630 at 363.346 kb on + strand, within RS_RS01630 at 363.347 kb on - strand, within RS_RS01630 at 363.520 kb on + strand, within RS_RS01630 at 363.521 kb on - strand, within RS_RS01630 at 363.521 kb on - strand, within RS_RS01630 at 365.465 kb on + strand, within RS_RS01635 at 365.465 kb on + strand, within RS_RS01635 at 365.465 kb on + strand, within RS_RS01635
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 361,776 + RS_RS01625 0.49 +0.7 361,825 - RS_RS01625 0.53 -0.2 361,909 - RS_RS01625 0.59 -0.0 361,909 - RS_RS01625 0.59 -2.2 362,067 + RS_RS01625 0.72 +0.0 362,067 + RS_RS01625 0.72 +0.7 362,067 + RS_RS01625 0.72 -0.4 362,067 + RS_RS01625 0.72 -0.4 362,068 - RS_RS01625 0.72 -0.2 362,068 - RS_RS01625 0.72 -0.1 362,068 - RS_RS01625 0.72 -0.0 362,482 + -0.1 362,483 - +1.6 362,483 - +0.2 362,483 - -0.0 362,791 + RS_RS01630 0.13 +0.4 362,830 + RS_RS01630 0.15 -0.0 362,917 + RS_RS01630 0.19 +0.6 362,959 + RS_RS01630 0.21 -0.7 362,960 - RS_RS01630 0.21 -0.2 362,960 - RS_RS01630 0.21 +0.5 363,002 - RS_RS01630 0.23 -0.5 363,035 - RS_RS01630 0.25 +1.6 363,089 - RS_RS01630 0.28 -0.8 363,122 - RS_RS01630 0.29 -1.5 363,124 + RS_RS01630 0.29 +0.2 363,125 - RS_RS01630 0.29 +0.3 363,125 - RS_RS01630 0.29 -0.5 363,125 - RS_RS01630 0.29 -0.6 363,286 + RS_RS01630 0.38 -0.7 363,346 + RS_RS01630 0.41 -2.2 363,347 - RS_RS01630 0.41 -0.1 363,520 + RS_RS01630 0.49 -0.4 363,521 - RS_RS01630 0.49 +0.7 363,521 - RS_RS01630 0.49 -1.2 365,465 + RS_RS01635 0.69 +0.6 365,465 + RS_RS01635 0.69 -0.6 365,465 + RS_RS01635 0.69 +0.2
Or see this region's nucleotide sequence