Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS00760 and RS_RS00765 overlap by 9 nucleotides RS_RS00765 and RS_RS00770 are separated by 17 nucleotides RS_RS00770 and RS_RS00775 are separated by 16 nucleotides
RS_RS00760: RS_RS00760 - MFS transporter, at 173,974 to 175,254
_RS00760
RS_RS00765: RS_RS00765 - class I SAM-dependent methyltransferase, at 175,246 to 176,439
_RS00765
RS_RS00770: RS_RS00770 - short chain dehydrogenase, at 176,457 to 177,260
_RS00770
RS_RS00775: RS_RS00775 - diguanylate cyclase, at 177,277 to 177,669
_RS00775
Position (kb) 175
176
177 Strain fitness (log2 ratio) -1
0
1
2 at 174.367 kb on + strand, within RS_RS00760 at 174.368 kb on - strand, within RS_RS00760 at 174.368 kb on - strand, within RS_RS00760 at 174.505 kb on + strand, within RS_RS00760 at 174.505 kb on + strand, within RS_RS00760 at 174.505 kb on + strand, within RS_RS00760 at 174.505 kb on + strand, within RS_RS00760 at 174.505 kb on + strand, within RS_RS00760 at 174.506 kb on - strand, within RS_RS00760 at 174.506 kb on - strand, within RS_RS00760 at 174.506 kb on - strand, within RS_RS00760 at 174.506 kb on - strand, within RS_RS00760 at 174.533 kb on + strand, within RS_RS00760 at 174.533 kb on + strand, within RS_RS00760 at 174.534 kb on - strand, within RS_RS00760 at 174.534 kb on - strand, within RS_RS00760 at 174.534 kb on - strand, within RS_RS00760 at 174.534 kb on - strand, within RS_RS00760 at 174.676 kb on + strand, within RS_RS00760 at 174.676 kb on + strand, within RS_RS00760 at 174.676 kb on + strand, within RS_RS00760 at 174.677 kb on - strand, within RS_RS00760 at 174.677 kb on - strand, within RS_RS00760 at 174.808 kb on + strand, within RS_RS00760 at 175.036 kb on + strand, within RS_RS00760 at 175.037 kb on - strand, within RS_RS00760 at 175.076 kb on - strand, within RS_RS00760 at 175.076 kb on - strand, within RS_RS00760 at 175.076 kb on - strand, within RS_RS00760 at 175.076 kb on - strand, within RS_RS00760 at 175.097 kb on - strand, within RS_RS00760 at 175.202 kb on - strand at 176.450 kb on + strand at 176.451 kb on - strand at 176.485 kb on + strand at 176.486 kb on - strand at 176.763 kb on + strand, within RS_RS00770 at 176.764 kb on - strand, within RS_RS00770 at 176.764 kb on - strand, within RS_RS00770 at 176.764 kb on - strand, within RS_RS00770 at 176.787 kb on + strand, within RS_RS00770 at 176.788 kb on - strand, within RS_RS00770 at 176.958 kb on + strand, within RS_RS00770 at 176.959 kb on - strand, within RS_RS00770 at 177.276 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 174,367 + RS_RS00760 0.31 +0.5 174,368 - RS_RS00760 0.31 -0.1 174,368 - RS_RS00760 0.31 +0.5 174,505 + RS_RS00760 0.41 +0.3 174,505 + RS_RS00760 0.41 +0.4 174,505 + RS_RS00760 0.41 +0.1 174,505 + RS_RS00760 0.41 +0.2 174,505 + RS_RS00760 0.41 +0.4 174,506 - RS_RS00760 0.42 -1.1 174,506 - RS_RS00760 0.42 +1.8 174,506 - RS_RS00760 0.42 -0.0 174,506 - RS_RS00760 0.42 +0.1 174,533 + RS_RS00760 0.44 +0.3 174,533 + RS_RS00760 0.44 +0.2 174,534 - RS_RS00760 0.44 +0.2 174,534 - RS_RS00760 0.44 +0.1 174,534 - RS_RS00760 0.44 +1.0 174,534 - RS_RS00760 0.44 -0.1 174,676 + RS_RS00760 0.55 +0.1 174,676 + RS_RS00760 0.55 +0.6 174,676 + RS_RS00760 0.55 +0.5 174,677 - RS_RS00760 0.55 +0.7 174,677 - RS_RS00760 0.55 +0.4 174,808 + RS_RS00760 0.65 +0.2 175,036 + RS_RS00760 0.83 +0.4 175,037 - RS_RS00760 0.83 +0.3 175,076 - RS_RS00760 0.86 +0.9 175,076 - RS_RS00760 0.86 +0.6 175,076 - RS_RS00760 0.86 +0.1 175,076 - RS_RS00760 0.86 +0.4 175,097 - RS_RS00760 0.88 +0.5 175,202 - +0.5 176,450 + -0.3 176,451 - +1.6 176,485 + +0.2 176,486 - +0.3 176,763 + RS_RS00770 0.38 -0.2 176,764 - RS_RS00770 0.38 +0.5 176,764 - RS_RS00770 0.38 +1.3 176,764 - RS_RS00770 0.38 +0.6 176,787 + RS_RS00770 0.41 +0.5 176,788 - RS_RS00770 0.41 +0.6 176,958 + RS_RS00770 0.62 -0.4 176,959 - RS_RS00770 0.62 +0.3 177,276 + +0.3
Or see this region's nucleotide sequence