Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS22870

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS22860 and RS_RS22865 are separated by 47 nucleotidesRS_RS22865 and RS_RS22870 are separated by 78 nucleotidesRS_RS22870 and RS_RS22875 are separated by 26 nucleotidesRS_RS22875 and RS_RS22880 are separated by 33 nucleotides RS_RS22860: RS_RS22860 - D-aminoacylase, at 1,508,867 to 1,510,351 _RS22860 RS_RS22865: RS_RS22865 - RidA family protein, at 1,510,399 to 1,510,785 _RS22865 RS_RS22870: RS_RS22870 - permease, at 1,510,864 to 1,512,216 _RS22870 RS_RS22875: RS_RS22875 - ketohydroxyglutarate aldolase, at 1,512,243 to 1,512,869 _RS22875 RS_RS22880: RS_RS22880 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 1,512,903 to 1,513,628 _RS22880 Position (kb) 1510 1511 1512 1513Strain fitness (log2 ratio) -1 0 1at 1510.771 kb on + strandat 1510.783 kb on + strandat 1510.927 kb on + strandat 1510.928 kb on - strandat 1511.384 kb on - strand, within RS_RS22870at 1511.414 kb on - strand, within RS_RS22870at 1511.716 kb on + strand, within RS_RS22870at 1511.716 kb on + strand, within RS_RS22870at 1511.717 kb on - strand, within RS_RS22870at 1512.110 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,510,771 + -0.5
1,510,783 + +0.7
1,510,927 + +0.9
1,510,928 - -1.2
1,511,384 - RS_RS22870 0.38 -1.4
1,511,414 - RS_RS22870 0.41 -1.4
1,511,716 + RS_RS22870 0.63 -0.3
1,511,716 + RS_RS22870 0.63 -0.1
1,511,717 - RS_RS22870 0.63 -1.1
1,512,110 - -1.7

Or see this region's nucleotide sequence