Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS16800 and RS_RS16805 are separated by 75 nucleotides RS_RS16805 and RS_RS16810 are separated by 139 nucleotides RS_RS16810 and RS_RS16815 are separated by 47 nucleotides RS_RS16815 and RS_RS16820 are separated by 15 nucleotides RS_RS16820 and RS_RS16825 are separated by 101 nucleotides
RS_RS16800: RS_RS16800 - lysoplasmalogenase, at 3,614,086 to 3,614,847
_RS16800
RS_RS16805: RS_RS16805 - Lrp/AsnC family transcriptional regulator, at 3,614,923 to 3,615,399
_RS16805
RS_RS16810: RS_RS16810 - phenylalanine 4-monooxygenase, at 3,615,539 to 3,616,480
_RS16810
RS_RS16815: RS_RS16815 - 4a-hydroxytetrahydrobiopterin dehydratase, at 3,616,528 to 3,616,833
_RS16815
RS_RS16820: RS_RS16820 - DUF3717 domain-containing protein, at 3,616,849 to 3,617,076
_RS16820
RS_RS16825: RS_RS16825 - SET domain-containing protein-lysine N-methyltransferase, at 3,617,178 to 3,617,717
_RS16825
Position (kb)
3615
3616
3617 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3614.745 kb on - strand, within RS_RS16800 at 3614.804 kb on + strand at 3614.982 kb on + strand, within RS_RS16805 at 3614.982 kb on + strand, within RS_RS16805 at 3615.080 kb on + strand, within RS_RS16805 at 3615.080 kb on + strand, within RS_RS16805 at 3615.080 kb on + strand, within RS_RS16805 at 3615.081 kb on - strand, within RS_RS16805 at 3615.081 kb on - strand, within RS_RS16805 at 3615.081 kb on - strand, within RS_RS16805 at 3615.104 kb on + strand, within RS_RS16805 at 3615.105 kb on - strand, within RS_RS16805 at 3615.105 kb on - strand, within RS_RS16805 at 3615.105 kb on - strand, within RS_RS16805 at 3615.498 kb on - strand at 3615.729 kb on - strand, within RS_RS16810 at 3615.975 kb on - strand, within RS_RS16810 at 3615.975 kb on - strand, within RS_RS16810 at 3616.049 kb on + strand, within RS_RS16810 at 3616.049 kb on + strand, within RS_RS16810 at 3616.050 kb on - strand, within RS_RS16810 at 3616.061 kb on + strand, within RS_RS16810 at 3616.062 kb on - strand, within RS_RS16810 at 3616.062 kb on - strand, within RS_RS16810 at 3616.121 kb on + strand, within RS_RS16810 at 3616.122 kb on - strand, within RS_RS16810 at 3616.122 kb on - strand, within RS_RS16810 at 3616.127 kb on + strand, within RS_RS16810 at 3616.127 kb on + strand, within RS_RS16810 at 3616.127 kb on + strand, within RS_RS16810 at 3616.128 kb on - strand, within RS_RS16810 at 3616.176 kb on - strand, within RS_RS16810 at 3616.215 kb on - strand, within RS_RS16810 at 3616.215 kb on - strand, within RS_RS16810 at 3616.307 kb on + strand, within RS_RS16810 at 3616.307 kb on + strand, within RS_RS16810 at 3616.307 kb on + strand, within RS_RS16810 at 3616.307 kb on + strand, within RS_RS16810 at 3616.307 kb on + strand, within RS_RS16810 at 3616.308 kb on - strand, within RS_RS16810 at 3616.370 kb on + strand, within RS_RS16810 at 3616.370 kb on + strand, within RS_RS16810 at 3616.370 kb on + strand, within RS_RS16810 at 3616.370 kb on + strand, within RS_RS16810 at 3616.371 kb on - strand, within RS_RS16810 at 3616.371 kb on - strand, within RS_RS16810 at 3616.474 kb on + strand at 3616.492 kb on - strand at 3616.832 kb on - strand at 3616.936 kb on + strand, within RS_RS16820 at 3616.936 kb on + strand, within RS_RS16820 at 3616.936 kb on + strand, within RS_RS16820 at 3616.936 kb on + strand, within RS_RS16820 at 3616.937 kb on - strand, within RS_RS16820 at 3616.953 kb on - strand at 3617.027 kb on + strand, within RS_RS16820 at 3617.027 kb on + strand, within RS_RS16820 at 3617.027 kb on + strand, within RS_RS16820 at 3617.087 kb on + strand at 3617.088 kb on - strand at 3617.088 kb on - strand at 3617.088 kb on - strand at 3617.302 kb on + strand, within RS_RS16825 at 3617.302 kb on + strand, within RS_RS16825 at 3617.308 kb on + strand, within RS_RS16825 at 3617.308 kb on + strand, within RS_RS16825 at 3617.308 kb on + strand, within RS_RS16825 at 3617.432 kb on - strand, within RS_RS16825
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 3,614,745 - RS_RS16800 0.86 +1.1 3,614,804 + +0.1 3,614,982 + RS_RS16805 0.12 +1.1 3,614,982 + RS_RS16805 0.12 +0.1 3,615,080 + RS_RS16805 0.33 -0.2 3,615,080 + RS_RS16805 0.33 -0.9 3,615,080 + RS_RS16805 0.33 -0.9 3,615,081 - RS_RS16805 0.33 +1.0 3,615,081 - RS_RS16805 0.33 +0.3 3,615,081 - RS_RS16805 0.33 +0.3 3,615,104 + RS_RS16805 0.38 +0.2 3,615,105 - RS_RS16805 0.38 +0.1 3,615,105 - RS_RS16805 0.38 +0.1 3,615,105 - RS_RS16805 0.38 +1.1 3,615,498 - +0.2 3,615,729 - RS_RS16810 0.20 -0.8 3,615,975 - RS_RS16810 0.46 +0.6 3,615,975 - RS_RS16810 0.46 -0.3 3,616,049 + RS_RS16810 0.54 +1.1 3,616,049 + RS_RS16810 0.54 -0.3 3,616,050 - RS_RS16810 0.54 +2.1 3,616,061 + RS_RS16810 0.55 -0.1 3,616,062 - RS_RS16810 0.56 -0.1 3,616,062 - RS_RS16810 0.56 -0.1 3,616,121 + RS_RS16810 0.62 -0.5 3,616,122 - RS_RS16810 0.62 -0.2 3,616,122 - RS_RS16810 0.62 +2.1 3,616,127 + RS_RS16810 0.62 -0.5 3,616,127 + RS_RS16810 0.62 -1.1 3,616,127 + RS_RS16810 0.62 +2.1 3,616,128 - RS_RS16810 0.63 -0.6 3,616,176 - RS_RS16810 0.68 +0.5 3,616,215 - RS_RS16810 0.72 -0.9 3,616,215 - RS_RS16810 0.72 +0.8 3,616,307 + RS_RS16810 0.82 +0.1 3,616,307 + RS_RS16810 0.82 +1.1 3,616,307 + RS_RS16810 0.82 +1.1 3,616,307 + RS_RS16810 0.82 +0.1 3,616,307 + RS_RS16810 0.82 -0.7 3,616,308 - RS_RS16810 0.82 +0.2 3,616,370 + RS_RS16810 0.88 -0.5 3,616,370 + RS_RS16810 0.88 -0.5 3,616,370 + RS_RS16810 0.88 +0.7 3,616,370 + RS_RS16810 0.88 -1.2 3,616,371 - RS_RS16810 0.88 -0.1 3,616,371 - RS_RS16810 0.88 +1.0 3,616,474 + +0.7 3,616,492 - -0.5 3,616,832 - +0.3 3,616,936 + RS_RS16820 0.38 +0.4 3,616,936 + RS_RS16820 0.38 +0.2 3,616,936 + RS_RS16820 0.38 -0.7 3,616,936 + RS_RS16820 0.38 -0.2 3,616,937 - RS_RS16820 0.39 +2.1 3,616,953 - +1.5 3,617,027 + RS_RS16820 0.78 -0.1 3,617,027 + RS_RS16820 0.78 -0.3 3,617,027 + RS_RS16820 0.78 -0.2 3,617,087 + -0.2 3,617,088 - -0.2 3,617,088 - +0.3 3,617,088 - -0.2 3,617,302 + RS_RS16825 0.23 -1.5 3,617,302 + RS_RS16825 0.23 +0.5 3,617,308 + RS_RS16825 0.24 -1.4 3,617,308 + RS_RS16825 0.24 +1.1 3,617,308 + RS_RS16825 0.24 +1.5 3,617,432 - RS_RS16825 0.47 -2.4
Or see this region's nucleotide sequence