Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS08750

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS08740 and RS_RS08745 are separated by 48 nucleotidesRS_RS08745 and RS_RS08750 are separated by 15 nucleotidesRS_RS08750 and RS_RS08755 are separated by 98 nucleotides RS_RS08740: RS_RS08740 - KR domain-containing protein, at 1,851,224 to 1,851,994 _RS08740 RS_RS08745: RS_RS08745 - ABC transporter permease, at 1,852,043 to 1,852,879 _RS08745 RS_RS08750: RS_RS08750 - ABC transporter permease, at 1,852,895 to 1,854,157 _RS08750 RS_RS08755: RS_RS08755 - ABC transporter substrate-binding protein, at 1,854,256 to 1,855,296 _RS08755 Position (kb) 1852 1853 1854 1855Strain fitness (log2 ratio) -1 0 1 2at 1852.446 kb on + strand, within RS_RS08745at 1852.483 kb on - strand, within RS_RS08745at 1852.782 kb on + strand, within RS_RS08745at 1852.942 kb on - strandat 1853.019 kb on + strandat 1853.028 kb on + strand, within RS_RS08750at 1853.028 kb on + strand, within RS_RS08750at 1853.032 kb on - strand, within RS_RS08750at 1853.032 kb on - strand, within RS_RS08750at 1853.032 kb on - strand, within RS_RS08750at 1853.032 kb on - strand, within RS_RS08750at 1853.080 kb on - strand, within RS_RS08750at 1853.083 kb on - strand, within RS_RS08750at 1853.148 kb on + strand, within RS_RS08750at 1853.148 kb on + strand, within RS_RS08750at 1853.149 kb on - strand, within RS_RS08750at 1853.149 kb on - strand, within RS_RS08750at 1853.149 kb on - strand, within RS_RS08750at 1853.149 kb on - strand, within RS_RS08750at 1853.149 kb on - strand, within RS_RS08750at 1853.149 kb on - strand, within RS_RS08750at 1853.149 kb on - strand, within RS_RS08750at 1853.205 kb on + strand, within RS_RS08750at 1853.235 kb on + strand, within RS_RS08750at 1853.281 kb on + strand, within RS_RS08750at 1853.282 kb on - strand, within RS_RS08750at 1853.282 kb on - strand, within RS_RS08750at 1853.282 kb on - strand, within RS_RS08750at 1853.284 kb on - strand, within RS_RS08750at 1853.284 kb on - strand, within RS_RS08750at 1853.284 kb on - strand, within RS_RS08750at 1853.562 kb on + strand, within RS_RS08750at 1853.562 kb on + strand, within RS_RS08750at 1853.563 kb on - strand, within RS_RS08750at 1853.563 kb on - strand, within RS_RS08750at 1853.563 kb on - strand, within RS_RS08750at 1853.637 kb on + strand, within RS_RS08750at 1853.898 kb on + strand, within RS_RS08750at 1854.258 kb on - strandat 1854.440 kb on + strand, within RS_RS08755at 1854.440 kb on + strand, within RS_RS08755at 1854.471 kb on - strand, within RS_RS08755at 1854.480 kb on - strand, within RS_RS08755at 1854.480 kb on - strand, within RS_RS08755at 1854.480 kb on - strand, within RS_RS08755at 1854.539 kb on + strand, within RS_RS08755at 1854.539 kb on + strand, within RS_RS08755at 1854.539 kb on + strand, within RS_RS08755at 1854.683 kb on + strand, within RS_RS08755at 1854.801 kb on - strand, within RS_RS08755at 1854.801 kb on - strand, within RS_RS08755at 1855.115 kb on + strand, within RS_RS08755at 1855.116 kb on - strand, within RS_RS08755

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,852,446 + RS_RS08745 0.48 -0.3
1,852,483 - RS_RS08745 0.53 +0.8
1,852,782 + RS_RS08745 0.88 -0.0
1,852,942 - +1.4
1,853,019 + +0.0
1,853,028 + RS_RS08750 0.11 -0.5
1,853,028 + RS_RS08750 0.11 +0.1
1,853,032 - RS_RS08750 0.11 +0.0
1,853,032 - RS_RS08750 0.11 -0.2
1,853,032 - RS_RS08750 0.11 +0.2
1,853,032 - RS_RS08750 0.11 -0.2
1,853,080 - RS_RS08750 0.15 +1.8
1,853,083 - RS_RS08750 0.15 -1.2
1,853,148 + RS_RS08750 0.20 +0.6
1,853,148 + RS_RS08750 0.20 +1.8
1,853,149 - RS_RS08750 0.20 +1.8
1,853,149 - RS_RS08750 0.20 -0.1
1,853,149 - RS_RS08750 0.20 +0.8
1,853,149 - RS_RS08750 0.20 +0.8
1,853,149 - RS_RS08750 0.20 +0.1
1,853,149 - RS_RS08750 0.20 -0.4
1,853,149 - RS_RS08750 0.20 -0.8
1,853,205 + RS_RS08750 0.25 -0.3
1,853,235 + RS_RS08750 0.27 +0.0
1,853,281 + RS_RS08750 0.31 +1.8
1,853,282 - RS_RS08750 0.31 -0.7
1,853,282 - RS_RS08750 0.31 +0.5
1,853,282 - RS_RS08750 0.31 +0.4
1,853,284 - RS_RS08750 0.31 +1.2
1,853,284 - RS_RS08750 0.31 -0.1
1,853,284 - RS_RS08750 0.31 +0.1
1,853,562 + RS_RS08750 0.53 +1.8
1,853,562 + RS_RS08750 0.53 +1.4
1,853,563 - RS_RS08750 0.53 -0.3
1,853,563 - RS_RS08750 0.53 +0.8
1,853,563 - RS_RS08750 0.53 +0.7
1,853,637 + RS_RS08750 0.59 -0.4
1,853,898 + RS_RS08750 0.79 +0.3
1,854,258 - -0.6
1,854,440 + RS_RS08755 0.18 -1.1
1,854,440 + RS_RS08755 0.18 +1.6
1,854,471 - RS_RS08755 0.21 +0.3
1,854,480 - RS_RS08755 0.22 -0.2
1,854,480 - RS_RS08755 0.22 -1.0
1,854,480 - RS_RS08755 0.22 +0.1
1,854,539 + RS_RS08755 0.27 -0.4
1,854,539 + RS_RS08755 0.27 -0.2
1,854,539 + RS_RS08755 0.27 +0.5
1,854,683 + RS_RS08755 0.41 -0.8
1,854,801 - RS_RS08755 0.52 -0.3
1,854,801 - RS_RS08755 0.52 +0.4
1,855,115 + RS_RS08755 0.83 +1.2
1,855,116 - RS_RS08755 0.83 +0.8

Or see this region's nucleotide sequence