Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS08275

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS08270 and RS_RS08275 are separated by 34 nucleotidesRS_RS08275 and RS_RS08280 are separated by 171 nucleotidesRS_RS08280 and RS_RS08285 overlap by 4 nucleotides RS_RS08270: RS_RS08270 - acetylornithine deacetylase, at 1,763,076 to 1,764,269 _RS08270 RS_RS08275: RS_RS08275 - transcription-repair coupling factor, at 1,764,304 to 1,767,735 _RS08275 RS_RS08280: RS_RS08280 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,767,907 to 1,768,668 _RS08280 RS_RS08285: RS_RS08285 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,768,665 to 1,769,171 _RS08285 Position (kb) 1764 1765 1766 1767 1768Strain fitness (log2 ratio) -2 -1 0 1 2at 1763.420 kb on - strand, within RS_RS08270at 1763.420 kb on - strand, within RS_RS08270at 1763.508 kb on - strand, within RS_RS08270at 1763.508 kb on - strand, within RS_RS08270at 1763.508 kb on - strand, within RS_RS08270at 1763.508 kb on - strand, within RS_RS08270at 1763.642 kb on - strand, within RS_RS08270at 1763.642 kb on - strand, within RS_RS08270at 1763.642 kb on - strand, within RS_RS08270at 1763.642 kb on - strand, within RS_RS08270at 1763.642 kb on - strand, within RS_RS08270at 1763.647 kb on - strand, within RS_RS08270at 1763.647 kb on - strand, within RS_RS08270at 1763.848 kb on + strand, within RS_RS08270at 1763.849 kb on - strand, within RS_RS08270at 1763.849 kb on - strand, within RS_RS08270at 1764.121 kb on + strand, within RS_RS08270at 1764.809 kb on + strand, within RS_RS08275at 1764.809 kb on + strand, within RS_RS08275at 1764.809 kb on + strand, within RS_RS08275at 1764.809 kb on + strand, within RS_RS08275at 1764.903 kb on - strand, within RS_RS08275at 1764.995 kb on + strand, within RS_RS08275at 1764.996 kb on - strand, within RS_RS08275at 1765.001 kb on + strand, within RS_RS08275at 1765.295 kb on + strand, within RS_RS08275at 1765.736 kb on + strand, within RS_RS08275at 1766.018 kb on + strand, within RS_RS08275at 1766.019 kb on - strand, within RS_RS08275at 1766.067 kb on + strand, within RS_RS08275at 1766.068 kb on - strand, within RS_RS08275at 1766.174 kb on + strand, within RS_RS08275at 1766.174 kb on + strand, within RS_RS08275at 1766.175 kb on - strand, within RS_RS08275at 1766.256 kb on - strand, within RS_RS08275at 1766.387 kb on + strand, within RS_RS08275at 1766.388 kb on - strand, within RS_RS08275at 1766.444 kb on + strand, within RS_RS08275at 1766.444 kb on + strand, within RS_RS08275at 1766.445 kb on - strand, within RS_RS08275at 1766.942 kb on + strand, within RS_RS08275at 1766.942 kb on + strandat 1766.942 kb on + strand, within RS_RS08275at 1766.943 kb on - strand, within RS_RS08275at 1766.943 kb on - strand, within RS_RS08275at 1766.945 kb on + strand, within RS_RS08275at 1766.946 kb on - strand, within RS_RS08275at 1766.987 kb on + strand, within RS_RS08275at 1766.988 kb on - strand, within RS_RS08275at 1766.988 kb on - strand, within RS_RS08275at 1767.272 kb on + strand, within RS_RS08275at 1767.342 kb on + strand, within RS_RS08275at 1767.342 kb on + strand, within RS_RS08275at 1767.342 kb on + strand, within RS_RS08275at 1767.342 kb on + strand, within RS_RS08275at 1767.343 kb on - strand, within RS_RS08275at 1767.343 kb on - strand, within RS_RS08275at 1767.819 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,763,420 - RS_RS08270 0.29 -1.8
1,763,420 - RS_RS08270 0.29 +1.8
1,763,508 - RS_RS08270 0.36 +0.2
1,763,508 - RS_RS08270 0.36 +0.1
1,763,508 - RS_RS08270 0.36 +0.1
1,763,508 - RS_RS08270 0.36 +1.8
1,763,642 - RS_RS08270 0.47 -2.0
1,763,642 - RS_RS08270 0.47 +0.4
1,763,642 - RS_RS08270 0.47 -0.2
1,763,642 - RS_RS08270 0.47 -0.3
1,763,642 - RS_RS08270 0.47 -0.5
1,763,647 - RS_RS08270 0.48 +1.1
1,763,647 - RS_RS08270 0.48 +0.0
1,763,848 + RS_RS08270 0.65 -1.2
1,763,849 - RS_RS08270 0.65 -0.1
1,763,849 - RS_RS08270 0.65 +0.8
1,764,121 + RS_RS08270 0.88 +1.8
1,764,809 + RS_RS08275 0.15 -1.4
1,764,809 + RS_RS08275 0.15 -1.4
1,764,809 + RS_RS08275 0.15 -0.5
1,764,809 + RS_RS08275 0.15 -2.0
1,764,903 - RS_RS08275 0.17 -0.8
1,764,995 + RS_RS08275 0.20 -1.3
1,764,996 - RS_RS08275 0.20 -0.0
1,765,001 + RS_RS08275 0.20 -0.8
1,765,295 + RS_RS08275 0.29 -1.6
1,765,736 + RS_RS08275 0.42 -2.5
1,766,018 + RS_RS08275 0.50 -2.3
1,766,019 - RS_RS08275 0.50 -2.4
1,766,067 + RS_RS08275 0.51 -1.5
1,766,068 - RS_RS08275 0.51 -1.2
1,766,174 + RS_RS08275 0.54 +0.9
1,766,174 + RS_RS08275 0.54 -0.5
1,766,175 - RS_RS08275 0.55 -2.4
1,766,256 - RS_RS08275 0.57 -1.4
1,766,387 + RS_RS08275 0.61 +0.8
1,766,388 - RS_RS08275 0.61 -1.9
1,766,444 + RS_RS08275 0.62 -1.5
1,766,444 + RS_RS08275 0.62 -1.7
1,766,445 - RS_RS08275 0.62 -0.5
1,766,942 + RS_RS08275 0.77 -1.7
1,766,942 + +1.8
1,766,942 + RS_RS08275 0.77 -0.5
1,766,943 - RS_RS08275 0.77 -2.2
1,766,943 - RS_RS08275 0.77 -2.6
1,766,945 + RS_RS08275 0.77 -1.2
1,766,946 - RS_RS08275 0.77 -2.0
1,766,987 + RS_RS08275 0.78 +0.2
1,766,988 - RS_RS08275 0.78 -1.7
1,766,988 - RS_RS08275 0.78 +0.8
1,767,272 + RS_RS08275 0.86 -1.5
1,767,342 + RS_RS08275 0.89 -1.7
1,767,342 + RS_RS08275 0.89 -1.6
1,767,342 + RS_RS08275 0.89 -0.8
1,767,342 + RS_RS08275 0.89 -1.4
1,767,343 - RS_RS08275 0.89 +1.8
1,767,343 - RS_RS08275 0.89 +0.8
1,767,819 + -0.5

Or see this region's nucleotide sequence