Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS05355

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS05345 and RS_RS05350 are separated by 30 nucleotidesRS_RS05350 and RS_RS05355 are separated by 392 nucleotidesRS_RS05355 and RS_RS05360 are separated by 108 nucleotides RS_RS05345: RS_RS05345 - DNA-binding response regulator, at 1,132,539 to 1,133,264 _RS05345 RS_RS05350: RS_RS05350 - GlsB/YeaQ/YmgE family stress response membrane protein, at 1,133,295 to 1,133,543 _RS05350 RS_RS05355: RS_RS05355 - FadR family transcriptional regulator, at 1,133,936 to 1,134,703 _RS05355 RS_RS05360: RS_RS05360 - glucarate dehydratase, at 1,134,812 to 1,136,164 _RS05360 Position (kb) 1133 1134 1135Strain fitness (log2 ratio) -1 0 1 2at 1132.945 kb on - strand, within RS_RS05345at 1132.945 kb on - strand, within RS_RS05345at 1133.106 kb on + strand, within RS_RS05345at 1133.107 kb on - strand, within RS_RS05345at 1133.107 kb on - strand, within RS_RS05345at 1133.123 kb on + strand, within RS_RS05345at 1133.124 kb on - strand, within RS_RS05345at 1133.124 kb on - strand, within RS_RS05345at 1133.124 kb on - strand, within RS_RS05345at 1133.518 kb on - strand, within RS_RS05350at 1133.518 kb on - strand, within RS_RS05350at 1133.650 kb on - strandat 1133.666 kb on + strandat 1133.743 kb on + strandat 1133.744 kb on - strandat 1133.776 kb on - strandat 1133.868 kb on + strandat 1133.870 kb on + strandat 1134.742 kb on + strandat 1134.833 kb on + strandat 1134.833 kb on + strandat 1134.834 kb on - strandat 1135.065 kb on - strand, within RS_RS05360at 1135.065 kb on - strand, within RS_RS05360at 1135.598 kb on + strand, within RS_RS05360

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,132,945 - RS_RS05345 0.56 -1.1
1,132,945 - RS_RS05345 0.56 -0.7
1,133,106 + RS_RS05345 0.78 -0.5
1,133,107 - RS_RS05345 0.78 +0.1
1,133,107 - RS_RS05345 0.78 -0.4
1,133,123 + RS_RS05345 0.80 +1.9
1,133,124 - RS_RS05345 0.81 -0.0
1,133,124 - RS_RS05345 0.81 -0.1
1,133,124 - RS_RS05345 0.81 +1.9
1,133,518 - RS_RS05350 0.90 +1.9
1,133,518 - RS_RS05350 0.90 +0.8
1,133,650 - -0.1
1,133,666 + -0.1
1,133,743 + -0.6
1,133,744 - -0.3
1,133,776 - -0.6
1,133,868 + -0.8
1,133,870 + +0.5
1,134,742 + -0.5
1,134,833 + +0.2
1,134,833 + -0.1
1,134,834 - -0.6
1,135,065 - RS_RS05360 0.19 -0.1
1,135,065 - RS_RS05360 0.19 -0.1
1,135,598 + RS_RS05360 0.58 -0.5

Or see this region's nucleotide sequence