Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS03095 and RS_RS03100 are separated by 529 nucleotides RS_RS03100 and RS_RS03105 are separated by 402 nucleotides
RS_RS03095: RS_RS03095 - hypothetical protein, at 663,924 to 664,280
_RS03095
RS_RS03100: RS_RS03100 - recombination-associated protein RdgC, at 664,810 to 665,733
_RS03100
RS_RS03105: RS_RS03105 - cytochrome b/b6 domain-containing protein, at 666,136 to 666,774
_RS03105
Position (kb) 664
665
666 Strain fitness (log2 ratio) -1
0
1
2
3 at 663.926 kb on - strand at 663.941 kb on - strand at 663.953 kb on - strand at 664.099 kb on + strand, within RS_RS03095 at 664.099 kb on + strand, within RS_RS03095 at 664.262 kb on + strand at 664.399 kb on - strand at 664.408 kb on + strand at 664.453 kb on - strand at 664.480 kb on + strand at 664.480 kb on + strand at 664.614 kb on - strand at 664.619 kb on + strand at 664.619 kb on + strand at 664.620 kb on - strand at 664.620 kb on - strand at 664.803 kb on + strand at 665.425 kb on + strand, within RS_RS03100 at 665.886 kb on + strand at 665.887 kb on - strand at 665.887 kb on - strand at 665.932 kb on - strand at 666.003 kb on - strand at 666.003 kb on - strand at 666.094 kb on + strand at 666.094 kb on + strand at 666.276 kb on + strand, within RS_RS03105 at 666.280 kb on + strand, within RS_RS03105 at 666.280 kb on + strand, within RS_RS03105 at 666.280 kb on + strand, within RS_RS03105 at 666.281 kb on - strand, within RS_RS03105 at 666.281 kb on - strand, within RS_RS03105 at 666.281 kb on - strand, within RS_RS03105 at 666.281 kb on - strand, within RS_RS03105 at 666.281 kb on - strand, within RS_RS03105 at 666.403 kb on + strand, within RS_RS03105 at 666.554 kb on - strand, within RS_RS03105 at 666.731 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 663,926 - -0.2 663,941 - +2.0 663,953 - +0.5 664,099 + RS_RS03095 0.49 +0.3 664,099 + RS_RS03095 0.49 +1.5 664,262 + -0.0 664,399 - +2.0 664,408 + +0.3 664,453 - -0.8 664,480 + +2.5 664,480 + -0.7 664,614 - -0.6 664,619 + +0.4 664,619 + +2.0 664,620 - -0.0 664,620 - -0.2 664,803 + -0.1 665,425 + RS_RS03100 0.67 +2.0 665,886 + +0.1 665,887 - -0.4 665,887 - +2.0 665,932 - -0.7 666,003 - -0.3 666,003 - -0.8 666,094 + -1.4 666,094 + -0.4 666,276 + RS_RS03105 0.22 +3.6 666,280 + RS_RS03105 0.23 -0.1 666,280 + RS_RS03105 0.23 +2.0 666,280 + RS_RS03105 0.23 +0.1 666,281 - RS_RS03105 0.23 -1.1 666,281 - RS_RS03105 0.23 -0.3 666,281 - RS_RS03105 0.23 +0.1 666,281 - RS_RS03105 0.23 +2.0 666,281 - RS_RS03105 0.23 -0.6 666,403 + RS_RS03105 0.42 +0.2 666,554 - RS_RS03105 0.65 +2.0 666,731 + +0.5
Or see this region's nucleotide sequence