Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS01095 and RS_RS01100 are separated by 45 nucleotides RS_RS01100 and RS_RS01105 are separated by 266 nucleotides RS_RS01105 and RS_RS01110 overlap by 4 nucleotides
RS_RS01095: RS_RS01095 - hypothetical protein, at 242,939 to 243,850
_RS01095
RS_RS01100: RS_RS01100 - sigma 54-dependent transcriptional regulator, at 243,896 to 245,503
_RS01100
RS_RS01105: RS_RS01105 - hypothetical protein, at 245,770 to 246,153
_RS01105
RS_RS01110: RS_RS01110 - RtcB family protein, at 246,150 to 247,370
_RS01110
Position (kb) 243
244
245
246 Strain fitness (log2 ratio) -2
-1
0
1
2 at 243.107 kb on - strand, within RS_RS01095 at 243.316 kb on + strand, within RS_RS01095 at 243.683 kb on + strand, within RS_RS01095 at 243.684 kb on - strand, within RS_RS01095 at 243.897 kb on + strand at 243.898 kb on - strand at 243.898 kb on - strand at 243.951 kb on + strand at 244.334 kb on - strand, within RS_RS01100 at 244.384 kb on - strand, within RS_RS01100 at 244.598 kb on + strand, within RS_RS01100 at 244.599 kb on - strand, within RS_RS01100 at 244.670 kb on + strand, within RS_RS01100 at 244.670 kb on + strand, within RS_RS01100 at 244.671 kb on - strand, within RS_RS01100 at 245.043 kb on + strand, within RS_RS01100 at 245.044 kb on - strand, within RS_RS01100 at 245.113 kb on - strand, within RS_RS01100 at 245.182 kb on - strand, within RS_RS01100 at 245.208 kb on + strand, within RS_RS01100 at 245.239 kb on - strand, within RS_RS01100 at 245.515 kb on + strand at 245.525 kb on + strand at 245.525 kb on + strand at 245.678 kb on + strand at 245.679 kb on - strand at 245.929 kb on + strand, within RS_RS01105 at 245.929 kb on + strand, within RS_RS01105 at 245.930 kb on - strand, within RS_RS01105 at 245.930 kb on - strand, within RS_RS01105 at 245.959 kb on + strand, within RS_RS01105 at 245.960 kb on - strand, within RS_RS01105 at 245.962 kb on + strand, within RS_RS01105 at 245.963 kb on - strand, within RS_RS01105 at 245.963 kb on - strand, within RS_RS01105 at 246.037 kb on + strand, within RS_RS01105 at 246.038 kb on - strand, within RS_RS01105 at 246.038 kb on - strand, within RS_RS01105 at 246.055 kb on + strand, within RS_RS01105 at 246.055 kb on + strand, within RS_RS01105 at 246.165 kb on + strand at 246.165 kb on + strand at 246.165 kb on + strand at 246.166 kb on - strand at 246.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 243,107 - RS_RS01095 0.18 +0.1 243,316 + RS_RS01095 0.41 +2.1 243,683 + RS_RS01095 0.82 +0.0 243,684 - RS_RS01095 0.82 -0.3 243,897 + -0.2 243,898 - +0.3 243,898 - +0.3 243,951 + +1.0 244,334 - RS_RS01100 0.27 -0.2 244,384 - RS_RS01100 0.30 +0.1 244,598 + RS_RS01100 0.44 -0.3 244,599 - RS_RS01100 0.44 -0.5 244,670 + RS_RS01100 0.48 -0.9 244,670 + RS_RS01100 0.48 -0.5 244,671 - RS_RS01100 0.48 +1.1 245,043 + RS_RS01100 0.71 +1.1 245,044 - RS_RS01100 0.71 -0.3 245,113 - RS_RS01100 0.76 -0.5 245,182 - RS_RS01100 0.80 +2.1 245,208 + RS_RS01100 0.82 -1.2 245,239 - RS_RS01100 0.84 -0.1 245,515 + -1.2 245,525 + +0.3 245,525 + +0.3 245,678 + -1.2 245,679 - +1.9 245,929 + RS_RS01105 0.41 +0.8 245,929 + RS_RS01105 0.41 -2.5 245,930 - RS_RS01105 0.42 +2.1 245,930 - RS_RS01105 0.42 +0.7 245,959 + RS_RS01105 0.49 +0.2 245,960 - RS_RS01105 0.49 -0.5 245,962 + RS_RS01105 0.50 +2.1 245,963 - RS_RS01105 0.50 -0.2 245,963 - RS_RS01105 0.50 +0.7 246,037 + RS_RS01105 0.70 +0.1 246,038 - RS_RS01105 0.70 -0.4 246,038 - RS_RS01105 0.70 +0.1 246,055 + RS_RS01105 0.74 +0.6 246,055 + RS_RS01105 0.74 -1.1 246,165 + -0.2 246,165 + +0.1 246,165 + +0.8 246,166 - -0.7 246,166 - -0.3
Or see this region's nucleotide sequence