Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS22465 and RS_RS22470 are separated by 90 nucleotides RS_RS22470 and RS_RS22475 are separated by 104 nucleotides RS_RS22475 and RS_RS22480 are separated by 281 nucleotides
RS_RS22465: RS_RS22465 - alpha/beta hydrolase, at 1,402,254 to 1,403,273
_RS22465
RS_RS22470: RS_RS22470 - hypothetical protein, at 1,403,364 to 1,404,137
_RS22470
RS_RS22475: RS_RS22475 - LysR family transcriptional regulator, at 1,404,242 to 1,405,189
_RS22475
RS_RS22480: RS_RS22480 - alpha/beta hydrolase, at 1,405,471 to 1,406,544
_RS22480
Position (kb)
1404
1405
1406 Strain fitness (log2 ratio)
-2
-1
0
1 at 1403.365 kb on + strand at 1403.366 kb on - strand at 1403.459 kb on + strand, within RS_RS22470 at 1403.459 kb on + strand, within RS_RS22470 at 1403.460 kb on - strand, within RS_RS22470 at 1403.492 kb on - strand, within RS_RS22470 at 1403.492 kb on - strand, within RS_RS22470 at 1403.800 kb on + strand, within RS_RS22470 at 1403.800 kb on + strand, within RS_RS22470 at 1403.800 kb on + strand, within RS_RS22470 at 1403.801 kb on - strand, within RS_RS22470 at 1403.801 kb on - strand, within RS_RS22470 at 1403.801 kb on - strand, within RS_RS22470 at 1403.801 kb on - strand, within RS_RS22470 at 1403.812 kb on + strand, within RS_RS22470 at 1403.999 kb on + strand, within RS_RS22470 at 1403.999 kb on + strand, within RS_RS22470 at 1404.373 kb on - strand, within RS_RS22475 at 1404.405 kb on + strand, within RS_RS22475 at 1404.405 kb on + strand, within RS_RS22475 at 1404.406 kb on - strand, within RS_RS22475 at 1404.406 kb on - strand, within RS_RS22475 at 1404.406 kb on - strand, within RS_RS22475 at 1404.406 kb on - strand, within RS_RS22475 at 1404.406 kb on - strand, within RS_RS22475 at 1404.406 kb on - strand, within RS_RS22475 at 1404.450 kb on + strand, within RS_RS22475 at 1404.450 kb on + strand, within RS_RS22475 at 1404.451 kb on - strand, within RS_RS22475 at 1404.615 kb on + strand, within RS_RS22475 at 1404.615 kb on + strand, within RS_RS22475 at 1404.616 kb on - strand, within RS_RS22475 at 1404.616 kb on - strand, within RS_RS22475 at 1404.616 kb on - strand, within RS_RS22475 at 1404.616 kb on - strand, within RS_RS22475 at 1404.616 kb on - strand, within RS_RS22475 at 1404.667 kb on - strand, within RS_RS22475 at 1404.678 kb on + strand, within RS_RS22475 at 1404.678 kb on + strand, within RS_RS22475 at 1404.679 kb on - strand, within RS_RS22475 at 1404.993 kb on + strand, within RS_RS22475 at 1404.993 kb on + strand, within RS_RS22475 at 1404.993 kb on + strand, within RS_RS22475 at 1404.994 kb on - strand, within RS_RS22475 at 1404.997 kb on - strand, within RS_RS22475 at 1405.324 kb on - strand at 1405.324 kb on - strand at 1405.366 kb on + strand at 1405.369 kb on + strand at 1405.369 kb on + strand at 1405.369 kb on + strand at 1405.370 kb on - strand at 1405.370 kb on - strand at 1405.370 kb on - strand at 1405.383 kb on + strand at 1405.394 kb on - strand at 1405.693 kb on + strand, within RS_RS22480 at 1405.694 kb on - strand, within RS_RS22480 at 1405.784 kb on + strand, within RS_RS22480 at 1405.795 kb on + strand, within RS_RS22480 at 1405.795 kb on + strand, within RS_RS22480 at 1405.796 kb on - strand, within RS_RS22480 at 1405.796 kb on - strand, within RS_RS22480 at 1405.796 kb on - strand, within RS_RS22480 at 1405.897 kb on + strand, within RS_RS22480 at 1405.945 kb on + strand, within RS_RS22480 at 1405.946 kb on - strand, within RS_RS22480 at 1405.946 kb on - strand, within RS_RS22480
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 1,403,365 + +0.0 1,403,366 - -0.2 1,403,459 + RS_RS22470 0.12 +0.2 1,403,459 + RS_RS22470 0.12 +0.1 1,403,460 - RS_RS22470 0.12 +0.5 1,403,492 - RS_RS22470 0.17 +0.5 1,403,492 - RS_RS22470 0.17 +0.1 1,403,800 + RS_RS22470 0.56 -0.3 1,403,800 + RS_RS22470 0.56 +0.6 1,403,800 + RS_RS22470 0.56 -0.3 1,403,801 - RS_RS22470 0.56 -1.1 1,403,801 - RS_RS22470 0.56 -0.1 1,403,801 - RS_RS22470 0.56 -0.3 1,403,801 - RS_RS22470 0.56 +1.0 1,403,812 + RS_RS22470 0.58 -0.4 1,403,999 + RS_RS22470 0.82 +0.0 1,403,999 + RS_RS22470 0.82 +0.4 1,404,373 - RS_RS22475 0.14 -0.9 1,404,405 + RS_RS22475 0.17 +0.5 1,404,405 + RS_RS22475 0.17 -0.5 1,404,406 - RS_RS22475 0.17 -1.0 1,404,406 - RS_RS22475 0.17 -1.0 1,404,406 - RS_RS22475 0.17 -0.6 1,404,406 - RS_RS22475 0.17 -0.8 1,404,406 - RS_RS22475 0.17 -1.8 1,404,406 - RS_RS22475 0.17 -0.5 1,404,450 + RS_RS22475 0.22 -0.5 1,404,450 + RS_RS22475 0.22 -0.9 1,404,451 - RS_RS22475 0.22 +0.0 1,404,615 + RS_RS22475 0.39 +0.0 1,404,615 + RS_RS22475 0.39 -1.8 1,404,616 - RS_RS22475 0.39 -0.7 1,404,616 - RS_RS22475 0.39 -0.7 1,404,616 - RS_RS22475 0.39 -1.2 1,404,616 - RS_RS22475 0.39 -1.3 1,404,616 - RS_RS22475 0.39 -1.0 1,404,667 - RS_RS22475 0.45 +0.2 1,404,678 + RS_RS22475 0.46 +0.0 1,404,678 + RS_RS22475 0.46 -1.5 1,404,679 - RS_RS22475 0.46 -1.2 1,404,993 + RS_RS22475 0.79 -2.1 1,404,993 + RS_RS22475 0.79 -1.7 1,404,993 + RS_RS22475 0.79 +0.0 1,404,994 - RS_RS22475 0.79 -1.0 1,404,997 - RS_RS22475 0.80 -0.7 1,405,324 - -0.4 1,405,324 - -0.4 1,405,366 + +0.4 1,405,369 + -0.2 1,405,369 + -0.3 1,405,369 + -0.3 1,405,370 - -1.4 1,405,370 - -0.2 1,405,370 - -0.7 1,405,383 + +1.3 1,405,394 - -0.7 1,405,693 + RS_RS22480 0.21 +0.2 1,405,694 - RS_RS22480 0.21 -0.5 1,405,784 + RS_RS22480 0.29 +1.0 1,405,795 + RS_RS22480 0.30 +1.3 1,405,795 + RS_RS22480 0.30 +1.0 1,405,796 - RS_RS22480 0.30 -1.1 1,405,796 - RS_RS22480 0.30 -0.0 1,405,796 - RS_RS22480 0.30 -0.1 1,405,897 + RS_RS22480 0.40 +0.2 1,405,945 + RS_RS22480 0.44 +0.6 1,405,946 - RS_RS22480 0.44 +1.0 1,405,946 - RS_RS22480 0.44 -1.3
Or see this region's nucleotide sequence