Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS15335 and RS_RS15340 are separated by 60 nucleotides RS_RS15340 and RS_RS15345 are separated by 190 nucleotides RS_RS15345 and RS_RS15350 overlap by 26 nucleotides
RS_RS15335: RS_RS15335 - glycerol kinase, at 3,289,517 to 3,291,013
_RS15335
RS_RS15340: RS_RS15340 - hypothetical protein, at 3,291,074 to 3,291,445
_RS15340
RS_RS15345: RS_RS15345 - DUF1415 domain-containing protein, at 3,291,636 to 3,292,262
_RS15345
RS_RS15350: RS_RS15350 - PIN domain-containing protein, at 3,292,237 to 3,292,650
_RS15350
Position (kb)
3291
3292 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3290.301 kb on - strand, within RS_RS15335 at 3290.301 kb on - strand, within RS_RS15335 at 3290.301 kb on - strand, within RS_RS15335 at 3290.301 kb on - strand, within RS_RS15335 at 3290.841 kb on - strand, within RS_RS15335 at 3290.841 kb on - strand, within RS_RS15335 at 3291.075 kb on + strand at 3291.088 kb on + strand at 3291.122 kb on + strand, within RS_RS15340 at 3291.122 kb on + strand, within RS_RS15340 at 3291.122 kb on + strand, within RS_RS15340 at 3291.122 kb on + strand, within RS_RS15340 at 3291.122 kb on + strand, within RS_RS15340 at 3291.123 kb on - strand, within RS_RS15340 at 3291.123 kb on - strand, within RS_RS15340 at 3291.123 kb on - strand, within RS_RS15340 at 3291.201 kb on + strand, within RS_RS15340 at 3291.202 kb on - strand, within RS_RS15340 at 3291.202 kb on - strand, within RS_RS15340 at 3291.234 kb on + strand, within RS_RS15340 at 3291.234 kb on + strand, within RS_RS15340 at 3291.234 kb on + strand, within RS_RS15340 at 3291.234 kb on + strand, within RS_RS15340 at 3291.234 kb on + strand, within RS_RS15340 at 3291.234 kb on + strand, within RS_RS15340 at 3291.234 kb on + strand, within RS_RS15340 at 3291.234 kb on + strand, within RS_RS15340 at 3291.234 kb on + strand, within RS_RS15340 at 3291.234 kb on + strand, within RS_RS15340 at 3291.234 kb on + strand, within RS_RS15340 at 3291.235 kb on - strand, within RS_RS15340 at 3291.235 kb on - strand, within RS_RS15340 at 3291.603 kb on + strand at 3291.603 kb on + strand at 3291.604 kb on - strand at 3292.170 kb on + strand, within RS_RS15345 at 3292.170 kb on + strand, within RS_RS15345 at 3292.170 kb on + strand, within RS_RS15345 at 3292.170 kb on + strand, within RS_RS15345 at 3292.170 kb on + strand, within RS_RS15345 at 3292.170 kb on + strand, within RS_RS15345 at 3292.170 kb on + strand, within RS_RS15345 at 3292.170 kb on + strand, within RS_RS15345 at 3292.170 kb on + strand, within RS_RS15345 at 3292.171 kb on - strand, within RS_RS15345 at 3292.171 kb on - strand, within RS_RS15345 at 3292.171 kb on - strand, within RS_RS15345 at 3292.171 kb on - strand, within RS_RS15345 at 3292.171 kb on - strand, within RS_RS15345 at 3292.171 kb on - strand, within RS_RS15345 at 3292.302 kb on + strand, within RS_RS15350 at 3292.313 kb on + strand, within RS_RS15350 at 3292.313 kb on + strand, within RS_RS15350 at 3292.313 kb on + strand, within RS_RS15350 at 3292.364 kb on + strand, within RS_RS15350 at 3292.365 kb on - strand, within RS_RS15350 at 3292.365 kb on - strand, within RS_RS15350 at 3292.365 kb on - strand, within RS_RS15350
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 3,290,301 - RS_RS15335 0.52 -0.9 3,290,301 - RS_RS15335 0.52 -0.1 3,290,301 - RS_RS15335 0.52 +1.4 3,290,301 - RS_RS15335 0.52 -0.4 3,290,841 - RS_RS15335 0.88 -0.5 3,290,841 - RS_RS15335 0.88 -0.7 3,291,075 + -3.0 3,291,088 + +0.0 3,291,122 + RS_RS15340 0.13 +0.3 3,291,122 + RS_RS15340 0.13 -0.5 3,291,122 + RS_RS15340 0.13 +0.4 3,291,122 + RS_RS15340 0.13 -0.4 3,291,122 + RS_RS15340 0.13 -0.1 3,291,123 - RS_RS15340 0.13 -0.4 3,291,123 - RS_RS15340 0.13 -0.3 3,291,123 - RS_RS15340 0.13 -0.1 3,291,201 + RS_RS15340 0.34 +0.0 3,291,202 - RS_RS15340 0.34 -0.4 3,291,202 - RS_RS15340 0.34 -0.4 3,291,234 + RS_RS15340 0.43 -0.1 3,291,234 + RS_RS15340 0.43 +0.5 3,291,234 + RS_RS15340 0.43 -0.1 3,291,234 + RS_RS15340 0.43 -0.9 3,291,234 + RS_RS15340 0.43 -0.9 3,291,234 + RS_RS15340 0.43 +1.4 3,291,234 + RS_RS15340 0.43 +0.4 3,291,234 + RS_RS15340 0.43 +0.3 3,291,234 + RS_RS15340 0.43 -0.3 3,291,234 + RS_RS15340 0.43 -0.1 3,291,234 + RS_RS15340 0.43 -0.3 3,291,235 - RS_RS15340 0.43 -0.2 3,291,235 - RS_RS15340 0.43 -0.1 3,291,603 + -0.9 3,291,603 + -0.3 3,291,604 - +0.2 3,292,170 + RS_RS15345 0.85 +0.5 3,292,170 + RS_RS15345 0.85 -0.4 3,292,170 + RS_RS15345 0.85 +0.0 3,292,170 + RS_RS15345 0.85 +0.1 3,292,170 + RS_RS15345 0.85 -0.4 3,292,170 + RS_RS15345 0.85 -0.1 3,292,170 + RS_RS15345 0.85 -0.1 3,292,170 + RS_RS15345 0.85 -0.6 3,292,170 + RS_RS15345 0.85 -1.0 3,292,171 - RS_RS15345 0.85 -0.0 3,292,171 - RS_RS15345 0.85 -0.9 3,292,171 - RS_RS15345 0.85 +1.4 3,292,171 - RS_RS15345 0.85 +1.9 3,292,171 - RS_RS15345 0.85 -0.6 3,292,171 - RS_RS15345 0.85 -0.4 3,292,302 + RS_RS15350 0.16 +1.0 3,292,313 + RS_RS15350 0.18 -0.1 3,292,313 + RS_RS15350 0.18 -0.1 3,292,313 + RS_RS15350 0.18 -1.0 3,292,364 + RS_RS15350 0.31 +0.4 3,292,365 - RS_RS15350 0.31 -0.1 3,292,365 - RS_RS15350 0.31 -0.6 3,292,365 - RS_RS15350 0.31 -0.1
Or see this region's nucleotide sequence