Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS14030

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS14025 and RS_RS14030 are separated by 436 nucleotidesRS_RS14030 and RS_RS14035 overlap by 8 nucleotides RS_RS14025: RS_RS14025 - ribonucleoside-diphosphate reductase subunit alpha, at 3,015,477 to 3,018,440 _RS14025 RS_RS14030: RS_RS14030 - 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD, at 3,018,877 to 3,019,479 _RS14030 RS_RS14035: RS_RS14035 - sigma-54-dependent Fis family transcriptional regulator, at 3,019,472 to 3,021,154 _RS14035 Position (kb) 3018 3019 3020Strain fitness (log2 ratio) -1 0 1at 3019.794 kb on + strand, within RS_RS14035at 3020.083 kb on - strand, within RS_RS14035at 3020.100 kb on - strand, within RS_RS14035at 3020.242 kb on - strand, within RS_RS14035

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
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3,019,794 + RS_RS14035 0.19 +1.3
3,020,083 - RS_RS14035 0.36 -1.5
3,020,100 - RS_RS14035 0.37 -1.5
3,020,242 - RS_RS14035 0.46 +1.3

Or see this region's nucleotide sequence