Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS05340 and RS_RS25420 are separated by 211 nucleotides RS_RS25420 and RS_RS05345 overlap by 4 nucleotides RS_RS05345 and RS_RS05350 are separated by 30 nucleotides RS_RS05350 and RS_RS05355 are separated by 392 nucleotides
RS_RS05340: RS_RS05340 - CHASE2 domain-containing protein, at 1,129,767 to 1,132,121
_RS05340
RS_RS25420: RS_RS25420 - hypothetical protein, at 1,132,333 to 1,132,542
_RS25420
RS_RS05345: RS_RS05345 - DNA-binding response regulator, at 1,132,539 to 1,133,264
_RS05345
RS_RS05350: RS_RS05350 - GlsB/YeaQ/YmgE family stress response membrane protein, at 1,133,295 to 1,133,543
_RS05350
RS_RS05355: RS_RS05355 - FadR family transcriptional regulator, at 1,133,936 to 1,134,703
_RS05355
Position (kb)
1132
1133
1134 Strain fitness (log2 ratio)
-1
0
1
2 at 1131.857 kb on - strand, within RS_RS05340 at 1132.228 kb on - strand at 1132.332 kb on + strand at 1132.332 kb on + strand at 1132.332 kb on + strand at 1132.332 kb on + strand at 1132.338 kb on - strand at 1132.359 kb on - strand, within RS_RS25420 at 1132.359 kb on - strand, within RS_RS25420 at 1132.390 kb on - strand, within RS_RS25420 at 1132.945 kb on - strand, within RS_RS05345 at 1132.945 kb on - strand, within RS_RS05345 at 1133.106 kb on + strand, within RS_RS05345 at 1133.107 kb on - strand, within RS_RS05345 at 1133.107 kb on - strand, within RS_RS05345 at 1133.123 kb on + strand, within RS_RS05345 at 1133.124 kb on - strand, within RS_RS05345 at 1133.124 kb on - strand, within RS_RS05345 at 1133.124 kb on - strand, within RS_RS05345 at 1133.518 kb on - strand, within RS_RS05350 at 1133.518 kb on - strand, within RS_RS05350 at 1133.650 kb on - strand at 1133.666 kb on + strand at 1133.743 kb on + strand at 1133.744 kb on - strand at 1133.776 kb on - strand at 1133.868 kb on + strand at 1133.870 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 1,131,857 - RS_RS05340 0.89 -0.1 1,132,228 - +1.0 1,132,332 + +0.2 1,132,332 + +0.1 1,132,332 + -0.5 1,132,332 + +0.6 1,132,338 - -0.7 1,132,359 - RS_RS25420 0.12 -0.8 1,132,359 - RS_RS25420 0.12 +0.3 1,132,390 - RS_RS25420 0.27 -0.1 1,132,945 - RS_RS05345 0.56 +0.8 1,132,945 - RS_RS05345 0.56 -0.2 1,133,106 + RS_RS05345 0.78 -0.1 1,133,107 - RS_RS05345 0.78 +0.3 1,133,107 - RS_RS05345 0.78 -0.3 1,133,123 + RS_RS05345 0.80 +2.2 1,133,124 - RS_RS05345 0.81 +0.1 1,133,124 - RS_RS05345 0.81 -0.9 1,133,124 - RS_RS05345 0.81 +1.2 1,133,518 - RS_RS05350 0.90 +1.2 1,133,518 - RS_RS05350 0.90 +0.3 1,133,650 - +1.8 1,133,666 + -0.8 1,133,743 + -0.1 1,133,744 - -0.3 1,133,776 - +0.2 1,133,868 + -0.4 1,133,870 + +0.1
Or see this region's nucleotide sequence