Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS01120 and RS_RS25270 are separated by 682 nucleotides RS_RS25270 and RS_RS01125 are separated by 326 nucleotides
RS_RS01120: RS_RS01120 - RNA 3'-terminal phosphate cyclase, at 248,347 to 249,390
_RS01120
RS_RS25270: RS_RS25270 - hypothetical protein, at 250,073 to 250,351
_RS25270
RS_RS01125: RS_RS01125 - PLP-dependent aminotransferase family protein, at 250,678 to 251,886
_RS01125
Position (kb) 250
251 Strain fitness (log2 ratio) -1
0
1
2
3 at 249.653 kb on + strand at 249.654 kb on - strand at 249.654 kb on - strand at 249.656 kb on - strand at 249.874 kb on - strand at 249.874 kb on - strand at 249.953 kb on + strand at 249.978 kb on + strand at 250.032 kb on + strand at 250.032 kb on + strand at 250.032 kb on + strand at 250.033 kb on - strand at 250.033 kb on - strand at 250.114 kb on + strand, within RS_RS25270 at 250.115 kb on - strand, within RS_RS25270 at 250.119 kb on + strand, within RS_RS25270 at 250.166 kb on - strand, within RS_RS25270 at 250.166 kb on - strand, within RS_RS25270 at 250.240 kb on + strand, within RS_RS25270 at 250.240 kb on + strand, within RS_RS25270 at 250.240 kb on + strand, within RS_RS25270 at 250.241 kb on - strand, within RS_RS25270 at 250.241 kb on - strand, within RS_RS25270 at 250.470 kb on - strand at 250.596 kb on + strand at 250.597 kb on - strand at 250.627 kb on + strand at 250.627 kb on + strand at 250.628 kb on - strand at 250.628 kb on - strand at 251.089 kb on + strand, within RS_RS01125 at 251.090 kb on - strand, within RS_RS01125 at 251.185 kb on + strand, within RS_RS01125 at 251.185 kb on + strand, within RS_RS01125 at 251.186 kb on - strand, within RS_RS01125 at 251.186 kb on - strand, within RS_RS01125 at 251.299 kb on + strand, within RS_RS01125 at 251.300 kb on - strand, within RS_RS01125 at 251.311 kb on + strand, within RS_RS01125 at 251.312 kb on - strand, within RS_RS01125 at 251.312 kb on - strand, within RS_RS01125 at 251.312 kb on - strand, within RS_RS01125
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 249,653 + -0.7 249,654 - -1.0 249,654 - +1.9 249,656 - -0.4 249,874 - -1.1 249,874 - +0.3 249,953 + +0.6 249,978 + -0.8 250,032 + -1.7 250,032 + +0.1 250,032 + +0.6 250,033 - -0.5 250,033 - -0.2 250,114 + RS_RS25270 0.15 +0.4 250,115 - RS_RS25270 0.15 +0.2 250,119 + RS_RS25270 0.16 -1.1 250,166 - RS_RS25270 0.33 -0.7 250,166 - RS_RS25270 0.33 -0.1 250,240 + RS_RS25270 0.60 +0.2 250,240 + RS_RS25270 0.60 -0.3 250,240 + RS_RS25270 0.60 -0.0 250,241 - RS_RS25270 0.60 -0.5 250,241 - RS_RS25270 0.60 +1.9 250,470 - +2.9 250,596 + -1.6 250,597 - -0.6 250,627 + -0.1 250,627 + +0.5 250,628 - -1.2 250,628 - -0.1 251,089 + RS_RS01125 0.34 +0.2 251,090 - RS_RS01125 0.34 -0.1 251,185 + RS_RS01125 0.42 +0.9 251,185 + RS_RS01125 0.42 -0.3 251,186 - RS_RS01125 0.42 +0.4 251,186 - RS_RS01125 0.42 -0.7 251,299 + RS_RS01125 0.51 -0.7 251,300 - RS_RS01125 0.51 -0.5 251,311 + RS_RS01125 0.52 +1.9 251,312 - RS_RS01125 0.52 +0.1 251,312 - RS_RS01125 0.52 +0.1 251,312 - RS_RS01125 0.52 -0.3
Or see this region's nucleotide sequence