Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS24225 and RS_RS24230 are separated by 324 nucleotides RS_RS24230 and RS_RS26465 are separated by 142 nucleotides RS_RS26465 and RS_RS24235 are separated by 156 nucleotides
RS_RS24225: RS_RS24225 - aldo/keto reductase, at 1,852,287 to 1,853,291
_RS24225
RS_RS24230: RS_RS24230 - hypothetical protein, at 1,853,616 to 1,853,810
_RS24230
RS_RS26465: RS_RS26465 - acyl-CoA dehydrogenase, at 1,853,953 to 1,854,179
_RS26465
RS_RS24235: RS_RS24235 - type III effector protein, at 1,854,336 to 1,855,445
_RS24235
Position (kb)
1853
1854 Strain fitness (log2 ratio)
-1
0
1 at 1852.621 kb on - strand, within RS_RS24225 at 1852.621 kb on - strand, within RS_RS24225 at 1852.621 kb on - strand, within RS_RS24225 at 1852.621 kb on - strand, within RS_RS24225 at 1852.621 kb on - strand, within RS_RS24225 at 1852.635 kb on + strand, within RS_RS24225 at 1852.636 kb on - strand, within RS_RS24225 at 1852.719 kb on + strand, within RS_RS24225 at 1852.720 kb on - strand, within RS_RS24225 at 1852.720 kb on - strand, within RS_RS24225 at 1852.873 kb on - strand, within RS_RS24225 at 1852.893 kb on + strand, within RS_RS24225 at 1852.929 kb on + strand, within RS_RS24225 at 1852.929 kb on + strand, within RS_RS24225 at 1852.929 kb on + strand, within RS_RS24225 at 1852.930 kb on - strand, within RS_RS24225 at 1853.157 kb on + strand, within RS_RS24225 at 1853.157 kb on + strand, within RS_RS24225 at 1853.158 kb on - strand, within RS_RS24225 at 1853.158 kb on - strand, within RS_RS24225 at 1853.247 kb on + strand at 1853.247 kb on + strand at 1853.248 kb on - strand at 1853.758 kb on + strand, within RS_RS24230 at 1853.758 kb on + strand, within RS_RS24230 at 1853.826 kb on - strand at 1854.063 kb on + strand, within RS_RS26465 at 1854.063 kb on + strand, within RS_RS26465 at 1854.063 kb on + strand, within RS_RS26465 at 1854.064 kb on - strand, within RS_RS26465 at 1854.064 kb on - strand, within RS_RS26465 at 1854.064 kb on - strand, within RS_RS26465 at 1854.064 kb on - strand, within RS_RS26465 at 1854.457 kb on + strand, within RS_RS24235 at 1854.458 kb on - strand, within RS_RS24235 at 1854.458 kb on - strand, within RS_RS24235 at 1854.493 kb on + strand, within RS_RS24235 at 1854.494 kb on - strand, within RS_RS24235 at 1854.494 kb on - strand, within RS_RS24235 at 1854.494 kb on - strand, within RS_RS24235
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 1,852,621 - RS_RS24225 0.33 +1.6 1,852,621 - RS_RS24225 0.33 +0.6 1,852,621 - RS_RS24225 0.33 -0.6 1,852,621 - RS_RS24225 0.33 -0.1 1,852,621 - RS_RS24225 0.33 -1.7 1,852,635 + RS_RS24225 0.35 +0.6 1,852,636 - RS_RS24225 0.35 +0.1 1,852,719 + RS_RS24225 0.43 -0.6 1,852,720 - RS_RS24225 0.43 -0.6 1,852,720 - RS_RS24225 0.43 +0.0 1,852,873 - RS_RS24225 0.58 +0.0 1,852,893 + RS_RS24225 0.60 +0.2 1,852,929 + RS_RS24225 0.64 -0.5 1,852,929 + RS_RS24225 0.64 -0.6 1,852,929 + RS_RS24225 0.64 -0.2 1,852,930 - RS_RS24225 0.64 -0.9 1,853,157 + RS_RS24225 0.87 -1.3 1,853,157 + RS_RS24225 0.87 +0.2 1,853,158 - RS_RS24225 0.87 -0.6 1,853,158 - RS_RS24225 0.87 -0.0 1,853,247 + -0.0 1,853,247 + -0.1 1,853,248 - -0.2 1,853,758 + RS_RS24230 0.73 +1.6 1,853,758 + RS_RS24230 0.73 -1.0 1,853,826 - +0.2 1,854,063 + RS_RS26465 0.48 -0.3 1,854,063 + RS_RS26465 0.48 -0.4 1,854,063 + RS_RS26465 0.48 -1.0 1,854,064 - RS_RS26465 0.49 -0.2 1,854,064 - RS_RS26465 0.49 +0.3 1,854,064 - RS_RS26465 0.49 +0.6 1,854,064 - RS_RS26465 0.49 +0.4 1,854,457 + RS_RS24235 0.11 -0.2 1,854,458 - RS_RS24235 0.11 -1.6 1,854,458 - RS_RS24235 0.11 +0.2 1,854,493 + RS_RS24235 0.14 -0.5 1,854,494 - RS_RS24235 0.14 +0.3 1,854,494 - RS_RS24235 0.14 -0.1 1,854,494 - RS_RS24235 0.14 -0.9
Or see this region's nucleotide sequence