Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS24185 and RS_RS24190 are separated by 192 nucleotides RS_RS24190 and RS_RS24195 are separated by 402 nucleotides RS_RS24195 and RS_RS24200 are separated by 277 nucleotides
RS_RS24185: RS_RS24185 - hypothetical protein, at 1,845,285 to 1,845,629
_RS24185
RS_RS24190: RS_RS24190 - hypothetical protein, at 1,845,822 to 1,846,151
_RS24190
RS_RS24195: RS_RS24195 - hypothetical protein, at 1,846,554 to 1,847,489
_RS24195
RS_RS24200: RS_RS24200 - protein phosphatase, at 1,847,767 to 1,848,411
_RS24200
Position (kb)
1846
1847
1848 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1845.604 kb on - strand at 1845.643 kb on + strand at 1845.660 kb on - strand at 1845.660 kb on - strand at 1845.794 kb on + strand at 1845.794 kb on + strand at 1845.827 kb on + strand at 1845.828 kb on - strand at 1845.945 kb on + strand, within RS_RS24190 at 1845.946 kb on - strand, within RS_RS24190 at 1845.946 kb on - strand, within RS_RS24190 at 1845.946 kb on - strand, within RS_RS24190 at 1845.946 kb on - strand, within RS_RS24190 at 1845.946 kb on - strand, within RS_RS24190 at 1845.946 kb on - strand, within RS_RS24190 at 1845.947 kb on + strand, within RS_RS24190 at 1845.948 kb on - strand, within RS_RS24190 at 1845.948 kb on - strand, within RS_RS24190 at 1845.987 kb on + strand, within RS_RS24190 at 1845.987 kb on + strand, within RS_RS24190 at 1846.023 kb on + strand, within RS_RS24190 at 1846.023 kb on + strand, within RS_RS24190 at 1846.023 kb on + strand, within RS_RS24190 at 1846.023 kb on + strand, within RS_RS24190 at 1846.024 kb on - strand, within RS_RS24190 at 1846.024 kb on - strand, within RS_RS24190 at 1846.024 kb on - strand, within RS_RS24190 at 1846.107 kb on + strand, within RS_RS24190 at 1846.107 kb on + strand, within RS_RS24190 at 1846.149 kb on + strand at 1846.150 kb on - strand at 1846.165 kb on + strand at 1846.221 kb on + strand at 1846.230 kb on - strand at 1846.461 kb on + strand at 1846.461 kb on + strand at 1846.461 kb on + strand at 1846.461 kb on + strand at 1846.462 kb on - strand at 1846.462 kb on - strand at 1846.738 kb on - strand, within RS_RS24195 at 1846.738 kb on - strand, within RS_RS24195 at 1846.738 kb on - strand, within RS_RS24195 at 1846.738 kb on - strand, within RS_RS24195 at 1846.810 kb on - strand, within RS_RS24195 at 1846.810 kb on - strand, within RS_RS24195 at 1846.810 kb on - strand, within RS_RS24195 at 1847.058 kb on + strand, within RS_RS24195 at 1847.058 kb on + strand, within RS_RS24195 at 1847.058 kb on + strand, within RS_RS24195 at 1847.059 kb on - strand, within RS_RS24195 at 1847.127 kb on + strand, within RS_RS24195 at 1847.127 kb on + strand, within RS_RS24195 at 1847.127 kb on + strand, within RS_RS24195 at 1847.128 kb on - strand, within RS_RS24195 at 1847.128 kb on - strand, within RS_RS24195 at 1847.128 kb on - strand, within RS_RS24195 at 1847.128 kb on - strand, within RS_RS24195 at 1847.128 kb on - strand, within RS_RS24195 at 1847.202 kb on + strand, within RS_RS24195 at 1847.203 kb on - strand, within RS_RS24195 at 1847.220 kb on + strand, within RS_RS24195 at 1847.281 kb on - strand, within RS_RS24195 at 1847.283 kb on + strand, within RS_RS24195 at 1847.284 kb on - strand, within RS_RS24195 at 1847.284 kb on - strand, within RS_RS24195 at 1847.284 kb on - strand, within RS_RS24195 at 1847.368 kb on + strand, within RS_RS24195 at 1847.368 kb on + strand, within RS_RS24195 at 1847.369 kb on - strand, within RS_RS24195 at 1847.370 kb on + strand, within RS_RS24195 at 1847.370 kb on + strand, within RS_RS24195 at 1847.370 kb on + strand, within RS_RS24195 at 1847.370 kb on + strand, within RS_RS24195 at 1847.371 kb on - strand, within RS_RS24195 at 1847.418 kb on + strand at 1847.418 kb on + strand at 1847.418 kb on + strand at 1847.418 kb on + strand at 1847.419 kb on - strand at 1847.419 kb on - strand at 1847.419 kb on - strand at 1847.503 kb on + strand at 1847.637 kb on + strand at 1847.676 kb on + strand at 1847.815 kb on + strand at 1847.833 kb on - strand, within RS_RS24200 at 1847.900 kb on + strand, within RS_RS24200 at 1847.925 kb on + strand, within RS_RS24200 at 1847.925 kb on + strand, within RS_RS24200 at 1847.940 kb on + strand, within RS_RS24200 at 1847.941 kb on - strand, within RS_RS24200 at 1847.951 kb on + strand, within RS_RS24200 at 1847.951 kb on + strand, within RS_RS24200 at 1847.952 kb on - strand, within RS_RS24200 at 1847.952 kb on - strand, within RS_RS24200 at 1848.079 kb on + strand, within RS_RS24200 at 1848.079 kb on + strand, within RS_RS24200 at 1848.233 kb on + strand, within RS_RS24200 at 1848.243 kb on + strand, within RS_RS24200 at 1848.325 kb on - strand, within RS_RS24200
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 1,845,604 - -0.3 1,845,643 + +0.1 1,845,660 - -0.0 1,845,660 - -0.1 1,845,794 + +1.6 1,845,794 + +0.3 1,845,827 + +0.1 1,845,828 - +0.2 1,845,945 + RS_RS24190 0.37 +0.2 1,845,946 - RS_RS24190 0.38 -1.0 1,845,946 - RS_RS24190 0.38 +0.0 1,845,946 - RS_RS24190 0.38 -0.4 1,845,946 - RS_RS24190 0.38 -0.8 1,845,946 - RS_RS24190 0.38 -0.7 1,845,946 - RS_RS24190 0.38 +1.6 1,845,947 + RS_RS24190 0.38 -0.0 1,845,948 - RS_RS24190 0.38 +0.0 1,845,948 - RS_RS24190 0.38 -0.0 1,845,987 + RS_RS24190 0.50 -0.6 1,845,987 + RS_RS24190 0.50 +1.6 1,846,023 + RS_RS24190 0.61 -0.0 1,846,023 + RS_RS24190 0.61 +0.3 1,846,023 + RS_RS24190 0.61 +0.0 1,846,023 + RS_RS24190 0.61 -0.2 1,846,024 - RS_RS24190 0.61 -0.2 1,846,024 - RS_RS24190 0.61 -0.3 1,846,024 - RS_RS24190 0.61 +0.4 1,846,107 + RS_RS24190 0.86 -0.1 1,846,107 + RS_RS24190 0.86 -0.2 1,846,149 + -0.4 1,846,150 - -0.4 1,846,165 + +1.0 1,846,221 + -0.4 1,846,230 - -1.1 1,846,461 + -0.8 1,846,461 + +0.6 1,846,461 + +0.2 1,846,461 + +0.6 1,846,462 - -0.4 1,846,462 - -0.0 1,846,738 - RS_RS24195 0.20 -2.1 1,846,738 - RS_RS24195 0.20 +0.6 1,846,738 - RS_RS24195 0.20 -0.8 1,846,738 - RS_RS24195 0.20 +1.6 1,846,810 - RS_RS24195 0.27 -0.8 1,846,810 - RS_RS24195 0.27 +0.1 1,846,810 - RS_RS24195 0.27 -0.3 1,847,058 + RS_RS24195 0.54 -0.9 1,847,058 + RS_RS24195 0.54 +1.6 1,847,058 + RS_RS24195 0.54 +0.5 1,847,059 - RS_RS24195 0.54 +0.2 1,847,127 + RS_RS24195 0.61 -1.8 1,847,127 + RS_RS24195 0.61 -0.4 1,847,127 + RS_RS24195 0.61 -0.2 1,847,128 - RS_RS24195 0.61 +1.6 1,847,128 - RS_RS24195 0.61 -0.2 1,847,128 - RS_RS24195 0.61 +1.6 1,847,128 - RS_RS24195 0.61 -0.5 1,847,128 - RS_RS24195 0.61 -0.2 1,847,202 + RS_RS24195 0.69 -0.6 1,847,203 - RS_RS24195 0.69 +0.8 1,847,220 + RS_RS24195 0.71 +0.2 1,847,281 - RS_RS24195 0.78 +1.6 1,847,283 + RS_RS24195 0.78 -0.8 1,847,284 - RS_RS24195 0.78 -0.5 1,847,284 - RS_RS24195 0.78 -0.2 1,847,284 - RS_RS24195 0.78 -0.3 1,847,368 + RS_RS24195 0.87 -0.6 1,847,368 + RS_RS24195 0.87 +1.6 1,847,369 - RS_RS24195 0.87 +0.6 1,847,370 + RS_RS24195 0.87 -1.4 1,847,370 + RS_RS24195 0.87 -0.1 1,847,370 + RS_RS24195 0.87 -0.5 1,847,370 + RS_RS24195 0.87 -0.8 1,847,371 - RS_RS24195 0.87 +0.5 1,847,418 + +0.0 1,847,418 + +0.4 1,847,418 + -1.1 1,847,418 + -0.4 1,847,419 - +0.1 1,847,419 - +2.6 1,847,419 - +1.6 1,847,503 + -0.2 1,847,637 + +1.0 1,847,676 + -1.2 1,847,815 + -0.4 1,847,833 - RS_RS24200 0.10 +0.1 1,847,900 + RS_RS24200 0.21 +1.0 1,847,925 + RS_RS24200 0.24 -0.3 1,847,925 + RS_RS24200 0.24 -0.5 1,847,940 + RS_RS24200 0.27 -0.3 1,847,941 - RS_RS24200 0.27 +0.5 1,847,951 + RS_RS24200 0.29 -0.9 1,847,951 + RS_RS24200 0.29 -0.7 1,847,952 - RS_RS24200 0.29 +1.6 1,847,952 - RS_RS24200 0.29 +1.6 1,848,079 + RS_RS24200 0.48 -0.3 1,848,079 + RS_RS24200 0.48 +0.1 1,848,233 + RS_RS24200 0.72 -0.6 1,848,243 + RS_RS24200 0.74 -0.5 1,848,325 - RS_RS24200 0.87 -0.3
Or see this region's nucleotide sequence