Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS22510

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS22505 and RS_RS26325 are separated by 82 nucleotidesRS_RS26325 and RS_RS22510 overlap by 66 nucleotidesRS_RS22510 and RS_RS22515 are separated by 48 nucleotidesRS_RS22515 and RS_RS22520 are separated by 77 nucleotides RS_RS22505: RS_RS22505 - low specificity L-threonine aldolase, at 1,414,162 to 1,415,259 _RS22505 RS_RS26325: RS_RS26325 - hypothetical protein, at 1,415,342 to 1,415,527 _RS26325 RS_RS22510: RS_RS22510 - hypothetical protein, at 1,415,462 to 1,416,139 _RS22510 RS_RS22515: RS_RS22515 - DUF2000 domain-containing protein, at 1,416,188 to 1,416,595 _RS22515 RS_RS22520: RS_RS22520 - transcriptional regulator, at 1,416,673 to 1,417,161 _RS22520 Position (kb) 1415 1416 1417Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1414.486 kb on + strand, within RS_RS22505at 1414.488 kb on + strand, within RS_RS22505at 1414.488 kb on + strand, within RS_RS22505at 1414.489 kb on - strand, within RS_RS22505at 1414.939 kb on + strand, within RS_RS22505at 1414.940 kb on - strand, within RS_RS22505at 1414.940 kb on - strand, within RS_RS22505at 1415.208 kb on + strandat 1415.209 kb on - strandat 1415.209 kb on - strandat 1415.209 kb on - strandat 1415.285 kb on + strandat 1415.335 kb on - strandat 1415.544 kb on - strand, within RS_RS22510at 1415.583 kb on + strand, within RS_RS22510at 1415.583 kb on + strand, within RS_RS22510at 1415.583 kb on + strand, within RS_RS22510at 1415.583 kb on + strand, within RS_RS22510at 1415.584 kb on - strand, within RS_RS22510at 1415.584 kb on - strand, within RS_RS22510at 1415.584 kb on - strand, within RS_RS22510at 1415.584 kb on - strand, within RS_RS22510at 1415.618 kb on - strand, within RS_RS22510at 1415.618 kb on - strand, within RS_RS22510at 1415.687 kb on + strand, within RS_RS22510at 1415.709 kb on + strand, within RS_RS22510at 1415.709 kb on + strand, within RS_RS22510at 1415.709 kb on + strand, within RS_RS22510at 1415.709 kb on + strand, within RS_RS22510at 1415.710 kb on - strand, within RS_RS22510at 1415.710 kb on - strand, within RS_RS22510at 1415.710 kb on - strand, within RS_RS22510at 1415.710 kb on - strand, within RS_RS22510at 1415.710 kb on - strand, within RS_RS22510at 1415.710 kb on - strand, within RS_RS22510at 1415.710 kb on - strand, within RS_RS22510at 1415.760 kb on + strand, within RS_RS22510at 1415.761 kb on - strand, within RS_RS22510at 1415.761 kb on - strand, within RS_RS22510at 1416.147 kb on - strandat 1416.157 kb on + strandat 1416.157 kb on + strandat 1416.158 kb on - strandat 1416.158 kb on - strandat 1416.158 kb on - strandat 1416.340 kb on + strand, within RS_RS22515at 1416.341 kb on - strand, within RS_RS22515at 1416.342 kb on + strand, within RS_RS22515at 1416.343 kb on - strand, within RS_RS22515at 1416.343 kb on - strand, within RS_RS22515at 1416.343 kb on - strand, within RS_RS22515at 1416.343 kb on - strand, within RS_RS22515at 1416.343 kb on - strand, within RS_RS22515at 1416.448 kb on - strand, within RS_RS22515at 1416.509 kb on - strand, within RS_RS22515at 1416.578 kb on + strandat 1416.579 kb on - strandat 1416.586 kb on + strandat 1416.587 kb on - strandat 1416.893 kb on + strand, within RS_RS22520at 1416.893 kb on + strand, within RS_RS22520at 1416.893 kb on + strand, within RS_RS22520at 1416.951 kb on - strand, within RS_RS22520at 1416.951 kb on - strand, within RS_RS22520at 1417.054 kb on + strand, within RS_RS22520at 1417.125 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
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1,414,486 + RS_RS22505 0.30 -0.1
1,414,488 + RS_RS22505 0.30 -2.3
1,414,488 + RS_RS22505 0.30 +0.2
1,414,489 - RS_RS22505 0.30 -0.6
1,414,939 + RS_RS22505 0.71 -0.7
1,414,940 - RS_RS22505 0.71 +0.2
1,414,940 - RS_RS22505 0.71 +0.1
1,415,208 + -0.3
1,415,209 - -0.6
1,415,209 - +0.9
1,415,209 - -0.9
1,415,285 + +0.4
1,415,335 - +1.7
1,415,544 - RS_RS22510 0.12 -1.0
1,415,583 + RS_RS22510 0.18 -0.2
1,415,583 + RS_RS22510 0.18 -1.9
1,415,583 + RS_RS22510 0.18 +0.5
1,415,583 + RS_RS22510 0.18 -0.0
1,415,584 - RS_RS22510 0.18 -0.7
1,415,584 - RS_RS22510 0.18 +1.4
1,415,584 - RS_RS22510 0.18 -0.6
1,415,584 - RS_RS22510 0.18 -1.0
1,415,618 - RS_RS22510 0.23 -0.2
1,415,618 - RS_RS22510 0.23 -0.4
1,415,687 + RS_RS22510 0.33 +1.4
1,415,709 + RS_RS22510 0.36 +0.6
1,415,709 + RS_RS22510 0.36 -0.2
1,415,709 + RS_RS22510 0.36 +0.4
1,415,709 + RS_RS22510 0.36 +0.4
1,415,710 - RS_RS22510 0.37 +1.4
1,415,710 - RS_RS22510 0.37 +0.1
1,415,710 - RS_RS22510 0.37 -1.0
1,415,710 - RS_RS22510 0.37 -0.3
1,415,710 - RS_RS22510 0.37 +0.0
1,415,710 - RS_RS22510 0.37 -0.1
1,415,710 - RS_RS22510 0.37 +0.3
1,415,760 + RS_RS22510 0.44 -0.4
1,415,761 - RS_RS22510 0.44 -1.3
1,415,761 - RS_RS22510 0.44 +0.2
1,416,147 - +0.6
1,416,157 + -0.3
1,416,157 + +0.1
1,416,158 - +1.0
1,416,158 - -0.2
1,416,158 - -0.2
1,416,340 + RS_RS22515 0.37 -0.2
1,416,341 - RS_RS22515 0.38 +3.3
1,416,342 + RS_RS22515 0.38 -0.1
1,416,343 - RS_RS22515 0.38 +0.0
1,416,343 - RS_RS22515 0.38 +2.2
1,416,343 - RS_RS22515 0.38 -0.2
1,416,343 - RS_RS22515 0.38 +1.4
1,416,343 - RS_RS22515 0.38 +0.5
1,416,448 - RS_RS22515 0.64 +0.7
1,416,509 - RS_RS22515 0.79 +0.6
1,416,578 + -0.0
1,416,579 - -0.6
1,416,586 + -0.4
1,416,587 - +0.4
1,416,893 + RS_RS22520 0.45 -0.2
1,416,893 + RS_RS22520 0.45 +0.5
1,416,893 + RS_RS22520 0.45 +1.4
1,416,951 - RS_RS22520 0.57 -1.4
1,416,951 - RS_RS22520 0.57 +0.4
1,417,054 + RS_RS22520 0.78 +0.8
1,417,125 + -0.4

Or see this region's nucleotide sequence