Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS17055 and RS_RS17060 overlap by 25 nucleotides RS_RS17060 and RS_RS17065 are separated by 36 nucleotides RS_RS17065 and RS_RS17070 are separated by 148 nucleotides
RS_RS17055: RS_RS17055 - two-component sensor histidine kinase, at 3,679,713 to 3,681,053
_RS17055
RS_RS17060: RS_RS17060 - DNA-binding response regulator, at 3,681,029 to 3,681,694
_RS17060
RS_RS17065: RS_RS17065 - membrane protein, at 3,681,731 to 3,682,516
_RS17065
RS_RS17070: RS_RS17070 - alpha/beta hydrolase, at 3,682,665 to 3,683,465
_RS17070
Position (kb)
3681
3682 Strain fitness (log2 ratio)
-1
0
1
2 at 3680.689 kb on - strand, within RS_RS17055 at 3680.695 kb on + strand, within RS_RS17055 at 3680.696 kb on - strand, within RS_RS17055 at 3680.696 kb on - strand, within RS_RS17055 at 3680.696 kb on - strand, within RS_RS17055 at 3680.701 kb on + strand, within RS_RS17055 at 3680.702 kb on - strand, within RS_RS17055 at 3681.156 kb on + strand, within RS_RS17060 at 3681.157 kb on - strand, within RS_RS17060 at 3681.402 kb on + strand, within RS_RS17060 at 3681.403 kb on - strand, within RS_RS17060 at 3681.561 kb on + strand, within RS_RS17060 at 3681.562 kb on - strand, within RS_RS17060 at 3681.621 kb on + strand, within RS_RS17060 at 3681.732 kb on + strand at 3681.733 kb on - strand at 3681.948 kb on + strand, within RS_RS17065 at 3681.949 kb on - strand, within RS_RS17065 at 3681.951 kb on + strand, within RS_RS17065 at 3681.951 kb on + strand, within RS_RS17065 at 3681.951 kb on + strand, within RS_RS17065 at 3681.952 kb on - strand, within RS_RS17065 at 3681.952 kb on - strand, within RS_RS17065 at 3681.952 kb on - strand, within RS_RS17065 at 3682.084 kb on - strand, within RS_RS17065 at 3682.200 kb on + strand, within RS_RS17065 at 3682.200 kb on + strand, within RS_RS17065 at 3682.278 kb on + strand, within RS_RS17065 at 3682.278 kb on + strand, within RS_RS17065 at 3682.293 kb on + strand, within RS_RS17065 at 3682.293 kb on + strand, within RS_RS17065 at 3682.570 kb on + strand at 3682.570 kb on + strand at 3682.570 kb on + strand at 3682.570 kb on + strand at 3682.570 kb on + strand at 3682.570 kb on + strand at 3682.570 kb on + strand at 3682.570 kb on + strand at 3682.571 kb on - strand at 3682.571 kb on - strand at 3682.571 kb on - strand at 3682.571 kb on - strand at 3682.571 kb on - strand at 3682.571 kb on - strand at 3682.571 kb on - strand at 3682.571 kb on - strand at 3682.621 kb on + strand at 3682.622 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 3,680,689 - RS_RS17055 0.73 -0.3 3,680,695 + RS_RS17055 0.73 -0.6 3,680,696 - RS_RS17055 0.73 -1.7 3,680,696 - RS_RS17055 0.73 -0.6 3,680,696 - RS_RS17055 0.73 -0.4 3,680,701 + RS_RS17055 0.74 -0.6 3,680,702 - RS_RS17055 0.74 -0.4 3,681,156 + RS_RS17060 0.19 +0.6 3,681,157 - RS_RS17060 0.19 -0.3 3,681,402 + RS_RS17060 0.56 +1.5 3,681,403 - RS_RS17060 0.56 -0.3 3,681,561 + RS_RS17060 0.80 +0.5 3,681,562 - RS_RS17060 0.80 -0.6 3,681,621 + RS_RS17060 0.89 -0.0 3,681,732 + -0.2 3,681,733 - -0.4 3,681,948 + RS_RS17065 0.28 -0.3 3,681,949 - RS_RS17065 0.28 -0.1 3,681,951 + RS_RS17065 0.28 -0.3 3,681,951 + RS_RS17065 0.28 -0.4 3,681,951 + RS_RS17065 0.28 -0.9 3,681,952 - RS_RS17065 0.28 -0.8 3,681,952 - RS_RS17065 0.28 -0.1 3,681,952 - RS_RS17065 0.28 +1.9 3,682,084 - RS_RS17065 0.45 -0.2 3,682,200 + RS_RS17065 0.60 -0.3 3,682,200 + RS_RS17065 0.60 +1.9 3,682,278 + RS_RS17065 0.70 -0.2 3,682,278 + RS_RS17065 0.70 +0.9 3,682,293 + RS_RS17065 0.72 +0.6 3,682,293 + RS_RS17065 0.72 +0.2 3,682,570 + -0.9 3,682,570 + +1.9 3,682,570 + +0.2 3,682,570 + -0.4 3,682,570 + +1.4 3,682,570 + -0.8 3,682,570 + +1.9 3,682,570 + +0.4 3,682,571 - +1.9 3,682,571 - -0.6 3,682,571 - +0.2 3,682,571 - -0.5 3,682,571 - -0.3 3,682,571 - +0.9 3,682,571 - -0.7 3,682,571 - +0.8 3,682,621 + -0.1 3,682,622 - +0.5
Or see this region's nucleotide sequence