Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS15365 and RS_RS15370 are separated by 7 nucleotides RS_RS15370 and RS_RS15375 are separated by 512 nucleotides
RS_RS15365: RS_RS15365 - AGE family epimerase/isomerase, at 3,294,747 to 3,295,979
_RS15365
RS_RS15370: RS_RS15370 - MFS transporter, at 3,295,987 to 3,297,282
_RS15370
RS_RS15375: RS_RS15375 - MFS transporter, at 3,297,795 to 3,299,096
_RS15375
Position (kb)
3295
3296
3297
3298 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3295.153 kb on - strand, within RS_RS15365 at 3295.368 kb on + strand, within RS_RS15365 at 3295.549 kb on - strand, within RS_RS15365 at 3295.740 kb on + strand, within RS_RS15365 at 3295.740 kb on + strand, within RS_RS15365 at 3295.741 kb on - strand, within RS_RS15365 at 3295.899 kb on + strand at 3296.046 kb on + strand at 3296.062 kb on + strand at 3296.062 kb on + strand at 3296.063 kb on - strand at 3296.063 kb on - strand at 3296.086 kb on + strand at 3296.086 kb on + strand at 3296.086 kb on + strand at 3296.087 kb on - strand at 3296.087 kb on - strand at 3296.213 kb on - strand, within RS_RS15370 at 3296.303 kb on - strand, within RS_RS15370 at 3296.399 kb on - strand, within RS_RS15370 at 3296.399 kb on - strand, within RS_RS15370 at 3296.741 kb on - strand, within RS_RS15370 at 3296.741 kb on - strand, within RS_RS15370 at 3297.019 kb on + strand, within RS_RS15370 at 3297.019 kb on + strand, within RS_RS15370 at 3297.020 kb on - strand, within RS_RS15370 at 3297.020 kb on - strand, within RS_RS15370 at 3297.095 kb on - strand, within RS_RS15370 at 3297.095 kb on - strand, within RS_RS15370 at 3297.623 kb on - strand at 3297.771 kb on + strand at 3297.771 kb on + strand at 3297.771 kb on + strand at 3297.772 kb on - strand at 3297.801 kb on - strand at 3297.801 kb on - strand at 3297.885 kb on + strand at 3297.891 kb on + strand at 3297.894 kb on + strand at 3297.894 kb on + strand at 3297.894 kb on + strand at 3297.895 kb on - strand at 3297.949 kb on + strand, within RS_RS15375 at 3297.956 kb on - strand, within RS_RS15375 at 3298.021 kb on - strand, within RS_RS15375 at 3298.021 kb on - strand, within RS_RS15375 at 3298.021 kb on - strand, within RS_RS15375 at 3298.207 kb on - strand, within RS_RS15375 at 3298.245 kb on + strand, within RS_RS15375 at 3298.245 kb on + strand, within RS_RS15375 at 3298.245 kb on + strand, within RS_RS15375 at 3298.246 kb on - strand, within RS_RS15375
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 3,295,153 - RS_RS15365 0.33 -0.9 3,295,368 + RS_RS15365 0.50 -2.3 3,295,549 - RS_RS15365 0.65 -0.9 3,295,740 + RS_RS15365 0.81 -3.6 3,295,740 + RS_RS15365 0.81 -3.6 3,295,741 - RS_RS15365 0.81 -3.7 3,295,899 + -5.7 3,296,046 + +1.9 3,296,062 + -0.4 3,296,062 + +0.2 3,296,063 - +0.2 3,296,063 - -0.2 3,296,086 + -0.0 3,296,086 + -2.6 3,296,086 + +0.5 3,296,087 - -1.3 3,296,087 - -0.3 3,296,213 - RS_RS15370 0.17 +0.3 3,296,303 - RS_RS15370 0.24 -0.1 3,296,399 - RS_RS15370 0.32 +0.9 3,296,399 - RS_RS15370 0.32 +0.9 3,296,741 - RS_RS15370 0.58 -0.1 3,296,741 - RS_RS15370 0.58 +0.3 3,297,019 + RS_RS15370 0.80 -1.0 3,297,019 + RS_RS15370 0.80 -0.1 3,297,020 - RS_RS15370 0.80 +1.9 3,297,020 - RS_RS15370 0.80 +1.9 3,297,095 - RS_RS15370 0.85 -1.3 3,297,095 - RS_RS15370 0.85 -0.1 3,297,623 - +0.2 3,297,771 + -0.2 3,297,771 + -0.5 3,297,771 + +1.2 3,297,772 - -0.9 3,297,801 - +0.9 3,297,801 - +0.4 3,297,885 + -0.2 3,297,891 + -0.5 3,297,894 + -0.2 3,297,894 + +0.3 3,297,894 + +1.9 3,297,895 - -0.2 3,297,949 + RS_RS15375 0.12 -0.7 3,297,956 - RS_RS15375 0.12 +0.9 3,298,021 - RS_RS15375 0.17 -1.1 3,298,021 - RS_RS15375 0.17 -0.2 3,298,021 - RS_RS15375 0.17 -0.7 3,298,207 - RS_RS15375 0.32 -1.0 3,298,245 + RS_RS15375 0.35 +1.5 3,298,245 + RS_RS15375 0.35 +0.1 3,298,245 + RS_RS15375 0.35 +1.9 3,298,246 - RS_RS15375 0.35 +0.3
Or see this region's nucleotide sequence