Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS15330

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS15320 and RS_RS15325 are separated by 22 nucleotidesRS_RS15325 and RS_RS15330 are separated by 156 nucleotidesRS_RS15330 and RS_RS15335 are separated by 161 nucleotides RS_RS15320: RS_RS15320 - carbohydrate ABC transporter permease, at 3,286,322 to 3,287,152 _RS15320 RS_RS15325: RS_RS15325 - membrane protein, at 3,287,175 to 3,287,456 _RS15325 RS_RS15330: RS_RS15330 - carbohydrate ABC transporter substrate-binding protein, at 3,287,613 to 3,289,355 _RS15330 RS_RS15335: RS_RS15335 - glycerol kinase, at 3,289,517 to 3,291,013 _RS15335 Position (kb) 3287 3288 3289 3290Strain fitness (log2 ratio) -1 0 1 2 3at 3286.715 kb on + strand, within RS_RS15320at 3286.716 kb on - strand, within RS_RS15320at 3286.860 kb on - strand, within RS_RS15320at 3287.117 kb on + strandat 3287.117 kb on + strandat 3287.118 kb on - strandat 3287.118 kb on - strandat 3287.118 kb on - strandat 3287.352 kb on + strand, within RS_RS15325at 3287.353 kb on - strand, within RS_RS15325at 3287.598 kb on + strandat 3287.608 kb on + strandat 3287.608 kb on + strandat 3287.609 kb on - strandat 3287.994 kb on + strand, within RS_RS15330at 3287.995 kb on - strand, within RS_RS15330at 3287.995 kb on - strand, within RS_RS15330at 3287.995 kb on - strand, within RS_RS15330at 3288.036 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.069 kb on + strand, within RS_RS15330at 3288.070 kb on - strand, within RS_RS15330at 3288.070 kb on - strand, within RS_RS15330at 3288.070 kb on - strand, within RS_RS15330at 3288.165 kb on + strand, within RS_RS15330at 3288.165 kb on + strand, within RS_RS15330at 3288.166 kb on - strand, within RS_RS15330at 3288.166 kb on - strand, within RS_RS15330at 3288.258 kb on + strand, within RS_RS15330at 3288.297 kb on + strand, within RS_RS15330at 3288.298 kb on - strand, within RS_RS15330at 3288.358 kb on - strand, within RS_RS15330at 3288.372 kb on + strand, within RS_RS15330at 3288.574 kb on - strand, within RS_RS15330at 3288.595 kb on - strand, within RS_RS15330at 3288.682 kb on - strand, within RS_RS15330at 3288.873 kb on + strand, within RS_RS15330at 3288.873 kb on + strand, within RS_RS15330at 3288.963 kb on + strand, within RS_RS15330at 3288.963 kb on + strand, within RS_RS15330at 3288.964 kb on - strand, within RS_RS15330at 3288.987 kb on + strand, within RS_RS15330at 3289.311 kb on + strandat 3289.432 kb on + strandat 3289.432 kb on + strandat 3289.432 kb on + strandat 3289.433 kb on - strandat 3289.517 kb on - strandat 3289.524 kb on - strandat 3290.058 kb on + strand, within RS_RS15335at 3290.058 kb on + strand, within RS_RS15335at 3290.059 kb on - strand, within RS_RS15335at 3290.301 kb on - strand, within RS_RS15335at 3290.301 kb on - strand, within RS_RS15335at 3290.301 kb on - strand, within RS_RS15335at 3290.301 kb on - strand, within RS_RS15335

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
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3,286,715 + RS_RS15320 0.47 -0.5
3,286,716 - RS_RS15320 0.47 -1.3
3,286,860 - RS_RS15320 0.65 +1.9
3,287,117 + -0.0
3,287,117 + -0.2
3,287,118 - +0.3
3,287,118 - -0.1
3,287,118 - -0.7
3,287,352 + RS_RS15325 0.63 +0.2
3,287,353 - RS_RS15325 0.63 -1.1
3,287,598 + +0.1
3,287,608 + +0.1
3,287,608 + +0.3
3,287,609 - -0.5
3,287,994 + RS_RS15330 0.22 -1.1
3,287,995 - RS_RS15330 0.22 -1.3
3,287,995 - RS_RS15330 0.22 -1.3
3,287,995 - RS_RS15330 0.22 -0.8
3,288,036 + RS_RS15330 0.24 -0.4
3,288,069 + RS_RS15330 0.26 +1.9
3,288,069 + RS_RS15330 0.26 -1.7
3,288,069 + RS_RS15330 0.26 -0.5
3,288,069 + RS_RS15330 0.26 -0.7
3,288,069 + RS_RS15330 0.26 -0.5
3,288,069 + RS_RS15330 0.26 +0.3
3,288,070 - RS_RS15330 0.26 -0.7
3,288,070 - RS_RS15330 0.26 -0.7
3,288,070 - RS_RS15330 0.26 -0.7
3,288,165 + RS_RS15330 0.32 -1.1
3,288,165 + RS_RS15330 0.32 -1.0
3,288,166 - RS_RS15330 0.32 -1.2
3,288,166 - RS_RS15330 0.32 -0.7
3,288,258 + RS_RS15330 0.37 -0.6
3,288,297 + RS_RS15330 0.39 -0.3
3,288,298 - RS_RS15330 0.39 +1.9
3,288,358 - RS_RS15330 0.43 -0.0
3,288,372 + RS_RS15330 0.44 -0.6
3,288,574 - RS_RS15330 0.55 -1.3
3,288,595 - RS_RS15330 0.56 +0.1
3,288,682 - RS_RS15330 0.61 -1.1
3,288,873 + RS_RS15330 0.72 -0.5
3,288,873 + RS_RS15330 0.72 +1.9
3,288,963 + RS_RS15330 0.77 +0.9
3,288,963 + RS_RS15330 0.77 -0.5
3,288,964 - RS_RS15330 0.78 -0.8
3,288,987 + RS_RS15330 0.79 -0.0
3,289,311 + -0.0
3,289,432 + -0.0
3,289,432 + +0.5
3,289,432 + +1.9
3,289,433 - -1.1
3,289,517 - -0.1
3,289,524 - -1.6
3,290,058 + RS_RS15335 0.36 -1.5
3,290,058 + RS_RS15335 0.36 -0.5
3,290,059 - RS_RS15335 0.36 -0.9
3,290,301 - RS_RS15335 0.52 -1.7
3,290,301 - RS_RS15335 0.52 -0.6
3,290,301 - RS_RS15335 0.52 +2.9
3,290,301 - RS_RS15335 0.52 -0.9

Or see this region's nucleotide sequence