Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS01110 and RS_RS01115 are separated by 88 nucleotides RS_RS01115 and RS_RS01120 are separated by 42 nucleotides
RS_RS01110: RS_RS01110 - RtcB family protein, at 246,150 to 247,370
_RS01110
RS_RS01115: RS_RS01115 - nucleotidyltransferase domain-containing protein, at 247,459 to 248,304
_RS01115
RS_RS01120: RS_RS01120 - RNA 3'-terminal phosphate cyclase, at 248,347 to 249,390
_RS01120
Position (kb) 247
248
249 Strain fitness (log2 ratio) -1
0
1
2 at 246.597 kb on + strand, within RS_RS01110 at 246.785 kb on + strand, within RS_RS01110 at 246.817 kb on - strand, within RS_RS01110 at 246.849 kb on + strand, within RS_RS01110 at 246.850 kb on - strand, within RS_RS01110 at 246.879 kb on + strand, within RS_RS01110 at 246.879 kb on + strand, within RS_RS01110 at 246.880 kb on - strand, within RS_RS01110 at 246.880 kb on - strand, within RS_RS01110 at 246.990 kb on + strand, within RS_RS01110 at 246.990 kb on + strand, within RS_RS01110 at 246.990 kb on + strand, within RS_RS01110 at 246.990 kb on + strand, within RS_RS01110 at 246.991 kb on - strand, within RS_RS01110 at 246.991 kb on - strand, within RS_RS01110 at 246.991 kb on - strand, within RS_RS01110 at 246.991 kb on - strand, within RS_RS01110 at 246.991 kb on - strand, within RS_RS01110 at 247.197 kb on + strand, within RS_RS01110 at 247.281 kb on + strand at 247.282 kb on - strand at 247.282 kb on - strand at 247.282 kb on - strand at 247.282 kb on - strand at 247.337 kb on + strand at 247.369 kb on - strand at 247.916 kb on - strand, within RS_RS01115 at 247.982 kb on - strand, within RS_RS01115 at 247.987 kb on + strand, within RS_RS01115 at 247.987 kb on + strand, within RS_RS01115 at 247.987 kb on + strand, within RS_RS01115 at 247.988 kb on - strand, within RS_RS01115 at 247.988 kb on - strand, within RS_RS01115 at 247.988 kb on - strand, within RS_RS01115 at 247.988 kb on - strand, within RS_RS01115 at 247.988 kb on - strand, within RS_RS01115 at 247.988 kb on - strand, within RS_RS01115 at 247.988 kb on - strand, within RS_RS01115 at 248.149 kb on + strand, within RS_RS01115 at 248.149 kb on + strand, within RS_RS01115 at 248.149 kb on + strand, within RS_RS01115 at 248.150 kb on - strand, within RS_RS01115 at 248.266 kb on + strand at 248.267 kb on - strand at 248.267 kb on - strand at 248.337 kb on + strand at 248.337 kb on + strand at 248.337 kb on + strand at 248.360 kb on + strand at 248.361 kb on - strand at 248.361 kb on - strand at 248.561 kb on + strand, within RS_RS01120 at 248.627 kb on - strand, within RS_RS01120 at 248.842 kb on + strand, within RS_RS01120
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 246,597 + RS_RS01110 0.37 -1.1 246,785 + RS_RS01110 0.52 -0.3 246,817 - RS_RS01110 0.55 +0.9 246,849 + RS_RS01110 0.57 +1.9 246,850 - RS_RS01110 0.57 +1.0 246,879 + RS_RS01110 0.60 -0.2 246,879 + RS_RS01110 0.60 +0.3 246,880 - RS_RS01110 0.60 -0.1 246,880 - RS_RS01110 0.60 +0.0 246,990 + RS_RS01110 0.69 -0.1 246,990 + RS_RS01110 0.69 -0.3 246,990 + RS_RS01110 0.69 -0.2 246,990 + RS_RS01110 0.69 -0.1 246,991 - RS_RS01110 0.69 +0.9 246,991 - RS_RS01110 0.69 -0.1 246,991 - RS_RS01110 0.69 -0.7 246,991 - RS_RS01110 0.69 +0.3 246,991 - RS_RS01110 0.69 +0.3 247,197 + RS_RS01110 0.86 -0.5 247,281 + -0.1 247,282 - -0.8 247,282 - +0.0 247,282 - -0.1 247,282 - +1.9 247,337 + +0.9 247,369 - -1.5 247,916 - RS_RS01115 0.54 -0.6 247,982 - RS_RS01115 0.62 +1.9 247,987 + RS_RS01115 0.62 +0.3 247,987 + RS_RS01115 0.62 +0.9 247,987 + RS_RS01115 0.62 -0.7 247,988 - RS_RS01115 0.63 +1.9 247,988 - RS_RS01115 0.63 +0.2 247,988 - RS_RS01115 0.63 +0.2 247,988 - RS_RS01115 0.63 -1.2 247,988 - RS_RS01115 0.63 -0.6 247,988 - RS_RS01115 0.63 +1.9 247,988 - RS_RS01115 0.63 +0.4 248,149 + RS_RS01115 0.82 +1.9 248,149 + RS_RS01115 0.82 -1.1 248,149 + RS_RS01115 0.82 -0.6 248,150 - RS_RS01115 0.82 -0.1 248,266 + -0.2 248,267 - -0.4 248,267 - -0.7 248,337 + -0.1 248,337 + -0.3 248,337 + -0.1 248,360 + -0.0 248,361 - +0.9 248,361 - +0.2 248,561 + RS_RS01120 0.20 -1.2 248,627 - RS_RS01120 0.27 -0.7 248,842 + RS_RS01120 0.47 +0.9
Or see this region's nucleotide sequence