Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS22475 and RS_RS22480 are separated by 281 nucleotides RS_RS22480 and RS_RS22485 are separated by 304 nucleotides
RS_RS22475: RS_RS22475 - LysR family transcriptional regulator, at 1,404,242 to 1,405,189
_RS22475
RS_RS22480: RS_RS22480 - alpha/beta hydrolase, at 1,405,471 to 1,406,544
_RS22480
RS_RS22485: RS_RS22485 - MexE family multidrug efflux RND transporter periplasmic adaptor subunit, at 1,406,849 to 1,408,165
_RS22485
Position (kb)
1405
1406
1407 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1404.615 kb on + strand, within RS_RS22475 at 1404.615 kb on + strand, within RS_RS22475 at 1404.616 kb on - strand, within RS_RS22475 at 1404.616 kb on - strand, within RS_RS22475 at 1404.616 kb on - strand, within RS_RS22475 at 1404.616 kb on - strand, within RS_RS22475 at 1404.616 kb on - strand, within RS_RS22475 at 1404.667 kb on - strand, within RS_RS22475 at 1404.678 kb on + strand, within RS_RS22475 at 1404.678 kb on + strand, within RS_RS22475 at 1404.679 kb on - strand, within RS_RS22475 at 1404.993 kb on + strand, within RS_RS22475 at 1404.993 kb on + strand, within RS_RS22475 at 1404.993 kb on + strand, within RS_RS22475 at 1404.994 kb on - strand, within RS_RS22475 at 1404.997 kb on - strand, within RS_RS22475 at 1405.324 kb on - strand at 1405.324 kb on - strand at 1405.366 kb on + strand at 1405.369 kb on + strand at 1405.369 kb on + strand at 1405.369 kb on + strand at 1405.370 kb on - strand at 1405.370 kb on - strand at 1405.370 kb on - strand at 1405.383 kb on + strand at 1405.394 kb on - strand at 1405.693 kb on + strand, within RS_RS22480 at 1405.694 kb on - strand, within RS_RS22480 at 1405.784 kb on + strand, within RS_RS22480 at 1405.795 kb on + strand, within RS_RS22480 at 1405.795 kb on + strand, within RS_RS22480 at 1405.796 kb on - strand, within RS_RS22480 at 1405.796 kb on - strand, within RS_RS22480 at 1405.796 kb on - strand, within RS_RS22480 at 1405.897 kb on + strand, within RS_RS22480 at 1405.945 kb on + strand, within RS_RS22480 at 1405.946 kb on - strand, within RS_RS22480 at 1405.946 kb on - strand, within RS_RS22480 at 1406.203 kb on + strand, within RS_RS22480 at 1406.203 kb on + strand, within RS_RS22480 at 1406.204 kb on - strand, within RS_RS22480 at 1406.237 kb on - strand, within RS_RS22480 at 1406.333 kb on - strand, within RS_RS22480 at 1406.656 kb on + strand at 1406.656 kb on + strand at 1406.657 kb on - strand at 1406.657 kb on - strand at 1406.678 kb on - strand at 1406.678 kb on - strand at 1406.761 kb on + strand at 1406.761 kb on + strand at 1406.762 kb on - strand at 1406.762 kb on - strand at 1406.918 kb on + strand at 1406.918 kb on + strand at 1406.918 kb on + strand at 1406.918 kb on + strand at 1406.919 kb on - strand at 1406.919 kb on - strand at 1406.919 kb on - strand at 1406.919 kb on - strand at 1406.919 kb on - strand at 1406.919 kb on - strand at 1406.920 kb on - strand at 1407.222 kb on - strand, within RS_RS22485 at 1407.500 kb on + strand, within RS_RS22485 at 1407.500 kb on + strand, within RS_RS22485 at 1407.500 kb on + strand, within RS_RS22485 at 1407.500 kb on + strand, within RS_RS22485 at 1407.501 kb on - strand, within RS_RS22485 at 1407.530 kb on + strand, within RS_RS22485 at 1407.530 kb on + strand, within RS_RS22485
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 1,404,615 + RS_RS22475 0.39 -0.0 1,404,615 + RS_RS22475 0.39 -1.3 1,404,616 - RS_RS22475 0.39 -1.1 1,404,616 - RS_RS22475 0.39 -0.7 1,404,616 - RS_RS22475 0.39 -1.4 1,404,616 - RS_RS22475 0.39 -1.4 1,404,616 - RS_RS22475 0.39 -1.6 1,404,667 - RS_RS22475 0.45 -0.3 1,404,678 + RS_RS22475 0.46 -0.0 1,404,678 + RS_RS22475 0.46 -1.6 1,404,679 - RS_RS22475 0.46 -1.0 1,404,993 + RS_RS22475 0.79 -1.4 1,404,993 + RS_RS22475 0.79 -1.2 1,404,993 + RS_RS22475 0.79 -0.0 1,404,994 - RS_RS22475 0.79 -1.1 1,404,997 - RS_RS22475 0.80 -0.7 1,405,324 - -0.0 1,405,324 - -0.5 1,405,366 + -0.1 1,405,369 + -0.8 1,405,369 + -0.2 1,405,369 + -0.3 1,405,370 - -2.5 1,405,370 - -1.8 1,405,370 - -1.2 1,405,383 + +0.2 1,405,394 - -0.7 1,405,693 + RS_RS22480 0.21 -0.7 1,405,694 - RS_RS22480 0.21 -0.6 1,405,784 + RS_RS22480 0.29 +0.5 1,405,795 + RS_RS22480 0.30 +0.7 1,405,795 + RS_RS22480 0.30 +1.0 1,405,796 - RS_RS22480 0.30 -0.6 1,405,796 - RS_RS22480 0.30 -0.3 1,405,796 - RS_RS22480 0.30 -0.5 1,405,897 + RS_RS22480 0.40 -0.3 1,405,945 + RS_RS22480 0.44 +0.2 1,405,946 - RS_RS22480 0.44 +1.0 1,405,946 - RS_RS22480 0.44 -0.4 1,406,203 + RS_RS22480 0.68 -0.6 1,406,203 + RS_RS22480 0.68 +0.9 1,406,204 - RS_RS22480 0.68 -2.3 1,406,237 - RS_RS22480 0.71 -1.3 1,406,333 - RS_RS22480 0.80 -1.1 1,406,656 + -0.0 1,406,656 + +0.5 1,406,657 - -1.7 1,406,657 - -0.9 1,406,678 - -1.6 1,406,678 - -1.4 1,406,761 + +0.0 1,406,761 + +0.5 1,406,762 - -3.1 1,406,762 - -0.0 1,406,918 + -1.1 1,406,918 + -1.2 1,406,918 + -1.6 1,406,918 + -1.4 1,406,919 - -0.9 1,406,919 - -0.8 1,406,919 - -1.5 1,406,919 - -0.6 1,406,919 - -1.0 1,406,919 - -0.7 1,406,920 - -0.9 1,407,222 - RS_RS22485 0.28 -2.4 1,407,500 + RS_RS22485 0.49 +0.4 1,407,500 + RS_RS22485 0.49 -1.3 1,407,500 + RS_RS22485 0.49 -1.6 1,407,500 + RS_RS22485 0.49 -2.2 1,407,501 - RS_RS22485 0.50 -0.5 1,407,530 + RS_RS22485 0.52 -0.6 1,407,530 + RS_RS22485 0.52 -1.5
Or see this region's nucleotide sequence