Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS22475

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS22465 and RS_RS22470 are separated by 90 nucleotidesRS_RS22470 and RS_RS22475 are separated by 104 nucleotidesRS_RS22475 and RS_RS22480 are separated by 281 nucleotides RS_RS22465: RS_RS22465 - alpha/beta hydrolase, at 1,402,254 to 1,403,273 _RS22465 RS_RS22470: RS_RS22470 - hypothetical protein, at 1,403,364 to 1,404,137 _RS22470 RS_RS22475: RS_RS22475 - LysR family transcriptional regulator, at 1,404,242 to 1,405,189 _RS22475 RS_RS22480: RS_RS22480 - alpha/beta hydrolase, at 1,405,471 to 1,406,544 _RS22480 Position (kb) 1404 1405 1406Strain fitness (log2 ratio) -2 -1 0 1at 1403.365 kb on + strandat 1403.366 kb on - strandat 1403.459 kb on + strand, within RS_RS22470at 1403.459 kb on + strand, within RS_RS22470at 1403.460 kb on - strand, within RS_RS22470at 1403.492 kb on - strand, within RS_RS22470at 1403.492 kb on - strand, within RS_RS22470at 1403.800 kb on + strand, within RS_RS22470at 1403.800 kb on + strand, within RS_RS22470at 1403.800 kb on + strand, within RS_RS22470at 1403.801 kb on - strand, within RS_RS22470at 1403.801 kb on - strand, within RS_RS22470at 1403.801 kb on - strand, within RS_RS22470at 1403.801 kb on - strand, within RS_RS22470at 1403.812 kb on + strand, within RS_RS22470at 1403.999 kb on + strand, within RS_RS22470at 1403.999 kb on + strand, within RS_RS22470at 1404.373 kb on - strand, within RS_RS22475at 1404.405 kb on + strand, within RS_RS22475at 1404.405 kb on + strand, within RS_RS22475at 1404.406 kb on - strand, within RS_RS22475at 1404.406 kb on - strand, within RS_RS22475at 1404.406 kb on - strand, within RS_RS22475at 1404.406 kb on - strand, within RS_RS22475at 1404.406 kb on - strand, within RS_RS22475at 1404.406 kb on - strand, within RS_RS22475at 1404.450 kb on + strand, within RS_RS22475at 1404.450 kb on + strand, within RS_RS22475at 1404.451 kb on - strand, within RS_RS22475at 1404.615 kb on + strand, within RS_RS22475at 1404.615 kb on + strand, within RS_RS22475at 1404.616 kb on - strand, within RS_RS22475at 1404.616 kb on - strand, within RS_RS22475at 1404.616 kb on - strand, within RS_RS22475at 1404.616 kb on - strand, within RS_RS22475at 1404.616 kb on - strand, within RS_RS22475at 1404.667 kb on - strand, within RS_RS22475at 1404.678 kb on + strand, within RS_RS22475at 1404.678 kb on + strand, within RS_RS22475at 1404.679 kb on - strand, within RS_RS22475at 1404.993 kb on + strand, within RS_RS22475at 1404.993 kb on + strand, within RS_RS22475at 1404.993 kb on + strand, within RS_RS22475at 1404.994 kb on - strand, within RS_RS22475at 1404.997 kb on - strand, within RS_RS22475at 1405.324 kb on - strandat 1405.324 kb on - strandat 1405.366 kb on + strandat 1405.369 kb on + strandat 1405.369 kb on + strandat 1405.369 kb on + strandat 1405.370 kb on - strandat 1405.370 kb on - strandat 1405.370 kb on - strandat 1405.383 kb on + strandat 1405.394 kb on - strandat 1405.693 kb on + strand, within RS_RS22480at 1405.694 kb on - strand, within RS_RS22480at 1405.784 kb on + strand, within RS_RS22480at 1405.795 kb on + strand, within RS_RS22480at 1405.795 kb on + strand, within RS_RS22480at 1405.796 kb on - strand, within RS_RS22480at 1405.796 kb on - strand, within RS_RS22480at 1405.796 kb on - strand, within RS_RS22480at 1405.897 kb on + strand, within RS_RS22480at 1405.945 kb on + strand, within RS_RS22480at 1405.946 kb on - strand, within RS_RS22480at 1405.946 kb on - strand, within RS_RS22480

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
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1,403,365 + +0.4
1,403,366 - -0.4
1,403,459 + RS_RS22470 0.12 +0.6
1,403,459 + RS_RS22470 0.12 +0.1
1,403,460 - RS_RS22470 0.12 -0.6
1,403,492 - RS_RS22470 0.17 -0.6
1,403,492 - RS_RS22470 0.17 +0.0
1,403,800 + RS_RS22470 0.56 -1.4
1,403,800 + RS_RS22470 0.56 +1.0
1,403,800 + RS_RS22470 0.56 +0.2
1,403,801 - RS_RS22470 0.56 -1.2
1,403,801 - RS_RS22470 0.56 -0.3
1,403,801 - RS_RS22470 0.56 -0.3
1,403,801 - RS_RS22470 0.56 +1.0
1,403,812 + RS_RS22470 0.58 +0.1
1,403,999 + RS_RS22470 0.82 +0.3
1,403,999 + RS_RS22470 0.82 -0.9
1,404,373 - RS_RS22475 0.14 -1.5
1,404,405 + RS_RS22475 0.17 +0.4
1,404,405 + RS_RS22475 0.17 -0.7
1,404,406 - RS_RS22475 0.17 -1.1
1,404,406 - RS_RS22475 0.17 -2.1
1,404,406 - RS_RS22475 0.17 -0.8
1,404,406 - RS_RS22475 0.17 -1.6
1,404,406 - RS_RS22475 0.17 -1.1
1,404,406 - RS_RS22475 0.17 -0.6
1,404,450 + RS_RS22475 0.22 -0.9
1,404,450 + RS_RS22475 0.22 -1.0
1,404,451 - RS_RS22475 0.22 -0.0
1,404,615 + RS_RS22475 0.39 -0.0
1,404,615 + RS_RS22475 0.39 -1.3
1,404,616 - RS_RS22475 0.39 -1.1
1,404,616 - RS_RS22475 0.39 -0.7
1,404,616 - RS_RS22475 0.39 -1.4
1,404,616 - RS_RS22475 0.39 -1.4
1,404,616 - RS_RS22475 0.39 -1.6
1,404,667 - RS_RS22475 0.45 -0.3
1,404,678 + RS_RS22475 0.46 -0.0
1,404,678 + RS_RS22475 0.46 -1.6
1,404,679 - RS_RS22475 0.46 -1.0
1,404,993 + RS_RS22475 0.79 -1.4
1,404,993 + RS_RS22475 0.79 -1.2
1,404,993 + RS_RS22475 0.79 -0.0
1,404,994 - RS_RS22475 0.79 -1.1
1,404,997 - RS_RS22475 0.80 -0.7
1,405,324 - -0.0
1,405,324 - -0.5
1,405,366 + -0.1
1,405,369 + -0.8
1,405,369 + -0.2
1,405,369 + -0.3
1,405,370 - -2.5
1,405,370 - -1.8
1,405,370 - -1.2
1,405,383 + +0.2
1,405,394 - -0.7
1,405,693 + RS_RS22480 0.21 -0.7
1,405,694 - RS_RS22480 0.21 -0.6
1,405,784 + RS_RS22480 0.29 +0.5
1,405,795 + RS_RS22480 0.30 +0.7
1,405,795 + RS_RS22480 0.30 +1.0
1,405,796 - RS_RS22480 0.30 -0.6
1,405,796 - RS_RS22480 0.30 -0.3
1,405,796 - RS_RS22480 0.30 -0.5
1,405,897 + RS_RS22480 0.40 -0.3
1,405,945 + RS_RS22480 0.44 +0.2
1,405,946 - RS_RS22480 0.44 +1.0
1,405,946 - RS_RS22480 0.44 -0.4

Or see this region's nucleotide sequence